{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T15:49:17Z","timestamp":1790783357916,"version":"4.1.0"},"reference-count":34,"publisher":"SAGE Publications","issue":"9","license":[{"start":{"date-parts":[[2013,2,1]],"date-time":"2013-02-01T00:00:00Z","timestamp":1359676800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Toxicol Ind Health"],"published-print":{"date-parts":[[2015,9]]},"abstract":"<jats:p>\n                    Carbonic anhydrase (CA) is a widely distributed enzyme and has a crucial role in the cells, tissues and organs of living organisms. It is found that CA-II is one of the most abundant CA isoenzymes in the gastrointestinal system. It plays an important role in the gastric acid secretion in stomach. In this study, we purified CA-II isoenzyme from sheep stomach with a 615.2 purification fold, 78% purification yield and 5562.02 specific activity. Moreover, the\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    effects of some commonly used pesticides including chlorpyrifos, cypermethrin, dichlorvos, glyphosate isopropylamine and lambda cyhalomethrin on the enzyme activity were investigated. Of these compounds, glyphosate isopropylamine and dichlorvos showed an inhibition on CA-II esterase activity. They have IC\n                    <jats:sub>50<\/jats:sub>\n                    values of 0.155\u00a0\u00b5M and 2.690\u00a0\u00b5M and K\n                    <jats:sub>i<\/jats:sub>\n                    values of 0.329\u00a0\u00b5M and 3.654\u00a0\u00b5M, respectively. Both glyphosate isopropylamine and dichlorvos inhibited CA-II isoenzyme in a noncompetitive manner.\n                  <\/jats:p>","DOI":"10.1177\/0748233713475508","type":"journal-article","created":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T12:13:10Z","timestamp":1441109590000},"page":"823-830","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":8,"title":["Influence of pesticide exposure on carbonic anhydrase II from sheep stomach"],"prefix":"10.1177","volume":"31","author":[{"given":"Nam\u0131k","family":"K\u0131l\u0131n\u00e7","sequence":"first","affiliation":[{"name":"Biochemistry Division, Faculty of Sciences, Department of Chemistry, Atat\u00fcrk University, Erzurum, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehmet Mustafa","family":"\u0130\u015fg\u00f6r","sequence":"additional","affiliation":[{"name":"Biochemistry Division, Faculty of Sciences, Department of Chemistry, Atat\u00fcrk University, Erzurum, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B\u00fclent","family":"\u015eeng\u00fcl","sequence":"additional","affiliation":[{"name":"Biochemistry Division, Faculty of Sciences, Department of Chemistry, Atat\u00fcrk University, Erzurum, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u015e\u00fckr\u00fc","family":"Beydemir","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2013,2]]},"reference":[{"key":"e_1_3_2_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2008.06.017"},{"key":"e_1_3_2_3_1","doi-asserted-by":"publisher","DOI":"10.1289\/ehp.97105s4801"},{"key":"e_1_3_2_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/14756360310001656736"},{"key":"e_1_3_2_5_1","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.25.966"},{"key":"e_1_3_2_6_1","doi-asserted-by":"publisher","DOI":"10.1006\/phrs.2000.0674"},{"key":"e_1_3_2_7_1","doi-asserted-by":"crossref","unstructured":"Bone Q Marshall NB Blaxter JHS (1995) In sensory systems and communication: biology of fishes Chapman. 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