{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:32:13Z","timestamp":1777923133135,"version":"3.51.4"},"reference-count":44,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2013,11,8]],"date-time":"2013-11-08T00:00:00Z","timestamp":1383868800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Toxicol Ind Health"],"published-print":{"date-parts":[[2016,4]]},"abstract":"<jats:p>\n                    This study aimed to investigate the antioxidant properties of different fractions obtained from the fruits of Lawsonia inermis, a widely used medicinal plant, against carbon tetrachloride (CCl\n                    <jats:sub>4<\/jats:sub>\n                    )-induced oxidative stress in rat liver. The results show that several fractions obtained from L. inermis fruits possessed important antioxidant activity. Among them, the ethyl acetate (EA) fraction showed the highest antioxidant activity. Then, EA fraction was selected for the purification of potential antioxidant compounds. The hepatoprotective effects of EA fraction and its most active constituent, gallic acid (GA), were evaluated against CCl\n                    <jats:sub>4<\/jats:sub>\n                    -induced hepatotoxicity in rats. CCl\n                    <jats:sub>4<\/jats:sub>\n                    induced oxidative stress by a significant rise in serum marker enzymes. However, pretreatment of rats with EA fraction of fruits of L. inermis at a dose of 250 mg kg\n                    <jats:sup>\u22121<\/jats:sup>\n                    body weight and GA significantly lowered some serum biochemical parameters (alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and lactate dehydrogenase) in treated rats. A significant reduction in hepatic thiobarbituric acid reactive substances and an increase in antioxidant enzymes namely superoxide dismutase, catalase, and glutathione peroxidase by treatment with plant extract and GA, against CCl\n                    <jats:sub>4<\/jats:sub>\n                    -treated rats, were observed. Histopathological examinations showed extensive liver injuries, characterized by extensive hepatocellular necrosis, vacuolization, and inflammatory cell infiltration. This potential antioxidant activity is comparable to those of the major purified antioxidant compound, GA. Based on these results, it was observed that fruits of L. inermis protect liver from oxidative stress induced by CCl\n                    <jats:sub>4<\/jats:sub>\n                    and thus help in evaluation of traditional claim on this plant.\n                  <\/jats:p>","DOI":"10.1177\/0748233713502839","type":"journal-article","created":{"date-parts":[[2013,11,8]],"date-time":"2013-11-08T23:15:05Z","timestamp":1383952505000},"page":"694-706","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":17,"title":["Protective effects of ethyl acetate fraction of\n                    <i>Lawsonia inermis<\/i>\n                    fruits extract against carbon tetrachloride-induced oxidative damage in rat liver"],"prefix":"10.1177","volume":"32","author":[{"given":"Anis Ben","family":"Hsouna","sequence":"first","affiliation":[{"name":"Biopesticides Team (LPIP), Center of Biotechnology of Sfax, Sfax, Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saoudi","family":"Mongi","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Ecophysiology, Faculty of Science, University of Sfax, Sfax, Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G\u00e9rald","family":"Culioli","sequence":"additional","affiliation":[{"name":"Laboratoire MAPIEM (EA 4323), Equipe Biofouling and Substances Naturelles Marines, Universit\u00e9 du Sud Toulon-Var, Toulon, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yves","family":"Blache","sequence":"additional","affiliation":[{"name":"Laboratoire MAPIEM (EA 4323), Equipe Biofouling and Substances Naturelles Marines, Universit\u00e9 du Sud Toulon-Var, Toulon, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zohra","family":"Ghlissi","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmacology, School of Medicine, University of Sfax, Sfax, Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rim","family":"Chaabane","sequence":"additional","affiliation":[{"name":"Biochemistry Laboratory, CHU Hedi Chaker of Sfax, Sfax, Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdelfattah","family":"El Feki","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Ecophysiology, Faculty of Science, University of Sfax, Sfax, Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samir","family":"Jaoua","sequence":"additional","affiliation":[{"name":"Biopesticides Team (LPIP), Center of Biotechnology of Sfax, Sfax, Tunisia"},{"name":"College of Arts and Sciences, University of Qatar, Doha, Qatar"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamed","family":"Trigui","sequence":"additional","affiliation":[{"name":"Biopesticides Team (LPIP), Center of Biotechnology of Sfax, Sfax, Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2013,11,8]]},"reference":[{"issue":"1","key":"bibr1-0748233713502839","first-page":"9","volume":"4","author":"Adesuyi AO","year":"2012","journal-title":"Advance Journal of Food Science and Technology"},{"key":"bibr2-0748233713502839","doi-asserted-by":"publisher","DOI":"10.1021\/jf030404l"},{"key":"bibr3-0748233713502839","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-091302-2.50032-3"},{"key":"bibr4-0748233713502839","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2006.06.011"},{"key":"bibr5-0748233713502839","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2007.09.009"},{"key":"bibr6-0748233713502839","first-page":"43","volume":"93","author":"Aziz NH","year":"1998","journal-title":"Microbios"},{"key":"bibr7-0748233713502839","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2010.08.060"},{"issue":"3","key":"bibr8-0748233713502839","first-page":"125","volume":"3","author":"Berenji F","year":"2010","journal-title":"Jundishapur Journal of Microbiology"},{"key":"bibr9-0748233713502839","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(87)90489-1"},{"key":"bibr10-0748233713502839","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2003.11.020"},{"key":"bibr11-0748233713502839","unstructured":"Bich DH, Chung DQ, Chuong BX, Dong NT, Dam DT, Hien PV, (2004) In the medicinal plants and animals in Vietnam. 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