{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:32:26Z","timestamp":1777923146090,"version":"3.51.4"},"reference-count":44,"publisher":"SAGE Publications","issue":"8","license":[{"start":{"date-parts":[[2015,1,22]],"date-time":"2015-01-22T00:00:00Z","timestamp":1421884800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Toxicol Ind Health"],"published-print":{"date-parts":[[2016,8]]},"abstract":"<jats:p>\n                    Dibromoacetonitrile (DBAN) is a disinfection by-product classified as a potential human and animal carcinogen. This study aimed at investigating the ability of myeloperoxidase (MPO) to oxidize DBAN to cyanide (CN\n                    <jats:sup>\u2212<\/jats:sup>\n                    ) in vitro. Detection of CN\n                    <jats:sup>\u2212<\/jats:sup>\n                    served as a marker for the possible generation of free radical intermediates implicated in DBAN-induced toxicity. Optimum conditions for the oxidation of DBAN to CN\n                    <jats:sup>\u2212<\/jats:sup>\n                    were characterized with respect to pH, temperature, and time of incubation as well as DBAN, MPO, potassium chloride, and hydrogen peroxide (H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    ) concentrations in incubation mixtures. Maximum reaction velocity and Michaelis\u2013Menten constant were assessed. Addition of sodium hypochlorite to the reaction mixtures significantly enhanced the rate of the reaction. Addition of the MPO inhibitors, sodium azide, 4-amino benzoic acid hydrazine, or indomethacin to the reaction mixtures significantly decreased the rate of DBAN oxidation. Inclusion of the antioxidant enzyme superoxide dismutase in the incubation mixtures significantly decreased the rate of reaction. Inclusion of the sulfhydryl compounds as reduced glutathione, N-acetylcysteine, d-penicillamine, or l-cysteine enhanced the rate of DBAN oxidation. These results demonstrate the ability of MPO\/H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    \/chloride ion system to oxidize DBAN to CN\n                    <jats:sup>\u2212<\/jats:sup>\n                    and provide insight for the elucidation of DBAN chronic toxicity.\n                  <\/jats:p>","DOI":"10.1177\/0748233714567184","type":"journal-article","created":{"date-parts":[[2015,1,23]],"date-time":"2015-01-23T00:51:32Z","timestamp":1421974292000},"page":"1478-1485","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["<i>In vitro<\/i>\n                    activation of dibromoacetonitrile to cyanide by myeloperoxidase"],"prefix":"10.1177","volume":"32","author":[{"given":"Fahad A","family":"Al-Abbasi","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2015,1,22]]},"reference":[{"key":"bibr1-0748233714567184","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxlet.2003.10.006"},{"key":"bibr2-0748233714567184","first-page":"376","volume-title":"Drug Metabolism and Disposition","volume":"8","author":"Abreu ME","year":"1980"},{"issue":"8","key":"bibr3-0748233714567184","first-page":"5","volume":"2","author":"Agner K","year":"1941","journal-title":"Acta Physiologica Scandinavica"},{"key":"bibr4-0748233714567184","first-page":"64A","volume":"15","author":"Agner K","year":"1958","journal-title":"Proceedings of the 4th International Congress on Biochemistry Vienna"},{"key":"bibr5-0748233714567184","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-08-016874-6.50042-4"},{"key":"bibr6-0748233714567184","doi-asserted-by":"publisher","DOI":"10.1016\/0041-008X(89)90313-X"},{"key":"bibr44-0748233714567184","doi-asserted-by":"publisher","DOI":"10.1016\/0300-483X(91)90029-Z"},{"issue":"24","key":"bibr7-0748233714567184","first-page":"211","volume":"26","author":"Aramini J","year":"2000","journal-title":"Canada Communicable Disease Report"},{"key":"bibr8-0748233714567184","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(01)94160-3"},{"key":"bibr9-0748233714567184","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2744(78)90197-3"},{"key":"bibr10-0748233714567184","first-page":"98","volume":"17","author":"Cotton RT","year":"1968","journal-title":"Journal of the Kansas Entomological Society"},{"key":"bibr11-0748233714567184","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-137-35782"},{"key":"bibr12-0748233714567184","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(93)90279-6"},{"key":"bibr13-0748233714567184","doi-asserted-by":"publisher","DOI":"10.1179\/135100000101535717"},{"issue":"2","key":"bibr14-0748233714567184","doi-asserted-by":"crossref","first-page":"570","DOI":"10.1016\/S0022-3565(25)20621-8","volume":"232","author":"Ghanayem BI","year":"1985","journal-title":"Journal of Pharmacology and Experimental Therapeutics"},{"key":"bibr15-0748233714567184","doi-asserted-by":"publisher","DOI":"10.3109\/03602539409029789"},{"key":"bibr16-0748233714567184","doi-asserted-by":"publisher","DOI":"10.1007\/BF01538126"},{"key":"bibr17-0748233714567184","doi-asserted-by":"publisher","DOI":"10.3109\/10408448509037460"},{"key":"bibr18-0748233714567184","first-page":"305","volume-title":"Cancer Enzymology","author":"Harrison JE","year":"1976"},{"key":"bibr19-0748233714567184","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)33749-3"},{"key":"bibr20-0748233714567184","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2004.10.019"},{"key":"bibr21-0748233714567184","first-page":"1","volume":"52","author":"IARC","year":"1991","journal-title":"IARC Monographs on the Evaluation of Carcinogenic Risks to Humans"},{"key":"bibr22-0748233714567184","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)89222-5"},{"key":"bibr23-0748233714567184","doi-asserted-by":"publisher","DOI":"10.1042\/bj2520529"},{"key":"bibr24-0748233714567184","doi-asserted-by":"publisher","DOI":"10.1042\/bj2630823"},{"key":"bibr25-0748233714567184","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4889(90)90146-5"},{"key":"bibr26-0748233714567184","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(91)90565-M"},{"key":"bibr27-0748233714567184","doi-asserted-by":"publisher","DOI":"10.1128\/JB.95.6.2131-2138.1968"},{"key":"bibr28-0748233714567184","first-page":"1","volume-title":"Peroxidases in Chemistry and Biology","author":"Klebanoff SJ","year":"1991"},{"key":"bibr29-0748233714567184","doi-asserted-by":"publisher","DOI":"10.1111\/paa.1999.111.5.383"},{"key":"bibr30-0748233714567184","first-page":"243","volume":"22","author":"Knobloch K","year":"1972","journal-title":"Medycyna Pracy"},{"key":"bibr31-0748233714567184","doi-asserted-by":"publisher","DOI":"10.1016\/S0162-0134(00)00213-0"},{"key":"bibr32-0748233714567184","doi-asserted-by":"publisher","DOI":"10.1007\/s00204-002-0418-7"},{"key":"bibr33-0748233714567184","doi-asserted-by":"publisher","DOI":"10.1021\/es0617441"},{"key":"bibr34-0748233714567184","first-page":"3","volume":"3","author":"Naskalski JW","year":"1995","journal-title":"Klinical Biochemistry and Metabolism"},{"key":"bibr35-0748233714567184","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2744(70)90083-5"},{"key":"bibr36-0748233714567184","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(94)00429-3"},{"key":"bibr37-0748233714567184","doi-asserted-by":"publisher","DOI":"10.1016\/0163-7258(95)02013-6"},{"key":"bibr38-0748233714567184","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4274(02)00072-3"},{"key":"bibr39-0748233714567184","doi-asserted-by":"publisher","DOI":"10.3109\/00498259009046911"},{"key":"bibr40-0748233714567184","doi-asserted-by":"publisher","DOI":"10.1017\/CBO9780511608438"},{"key":"bibr41-0748233714567184","first-page":"83","volume-title":"Peroxidases in Chemistry and Biology","author":"Thomas EL","year":"1991"},{"key":"bibr42-0748233714567184","first-page":"941","volume-title":"Advances in the Identification and Analysis of Organic Pollutants in Water","author":"Trehy ML","year":"1981"},{"key":"bibr43-0748233714567184","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2006.12.004"}],"container-title":["Toxicology and Industrial Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0748233714567184","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/0748233714567184","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0748233714567184","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T20:40:19Z","timestamp":1777668019000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/0748233714567184"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,1,22]]},"references-count":44,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2016,8]]}},"alternative-id":["10.1177\/0748233714567184"],"URL":"https:\/\/doi.org\/10.1177\/0748233714567184","relation":{},"ISSN":["0748-2337","1477-0393"],"issn-type":[{"value":"0748-2337","type":"print"},{"value":"1477-0393","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,1,22]]}}}