{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:32:57Z","timestamp":1777923177197,"version":"3.51.4"},"reference-count":30,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2017,11,13]],"date-time":"2017-11-13T00:00:00Z","timestamp":1510531200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Toxicol Ind Health"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:p>\n                    To investigate the expression profile of monocyte chemoattractant peptide-1 (MCP-1) by keratinocytes after nickel exposure and to identify its role for leucocyte migration during nickel-induced occupational allergic contact dermatitis (OACD), 26 workers diagnosed with nickel-induced OACD were enrolled. Skin biopsies from the positive nickel-challenged sites at different time points were assessed by immunohistochemistry (IHC) for MCP-1, CD68, CD45RO, and in situ hybridization (ISH) for MCP-1, using chronic periumbilical dermititis as controls. The expressions of MCP-1 in HaCaT cell culture after nickel treatment were quantified by enzyme-linked immunosorbent assay. The results showed that at positive nickel-challenged sites, strong expressions of MCP-1, both messenger RNA (mRNA) and protein, were detected in the basal keratinocytes during the early phase (24\u201348 h after nickel application), paralleled by the recruitment of CD68\n                    <jats:sup>+<\/jats:sup>\n                    and CD45RO\n                    <jats:sup>+<\/jats:sup>\n                    cells to the skin compartments. The expressions of MCP-1 declined gradually in the late phase (72\u201396 h after nickel application). Treatment with nickel sulfate at noncytotoxic concentrations (0.01\u2013100 \u00b5M) induced a concentration-related elevation of MCP-1 expression by HaCaT cells compared to the untreated cells. The data indicated that a temporal expression pattern of MCP-1 produced by keratinocytes after nickel exposure was involved in the complex process of mononuclear cell infiltration during elicitation of nickel-induced OACD. Targeting MCP-1 might be a potential therapeutic strategy for OACD.\n                  <\/jats:p>","DOI":"10.1177\/0748233717738633","type":"journal-article","created":{"date-parts":[[2017,11,13]],"date-time":"2017-11-13T20:28:33Z","timestamp":1510604913000},"page":"36-43","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":13,"title":["MCP-1 produced by keratinocytes is associated with leucocyte recruitment during elicitation of nickel-induced occupational allergic contact dermatitis"],"prefix":"10.1177","volume":"34","author":[{"given":"Weihua","family":"Mai","sequence":"first","affiliation":[{"name":"Department of Preventive Medicine, The Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai, Guangdong, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dongqing","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Dermatology, The Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai, Guangdong, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xingwei","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of General Surgery, The Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai, Guangdong, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ling","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Medical Quality Control, The Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai, Guangdong, 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