{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:36:29Z","timestamp":1777923389551,"version":"3.51.4"},"reference-count":9,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[1988,1,1]],"date-time":"1988-01-01T00:00:00Z","timestamp":567993600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxicol Ind Health"],"published-print":{"date-parts":[[1988,1]]},"abstract":"<jats:p>A rapid, inexpensive beryllium acute toxicity assay using human erythrocyte ATP levels has been developed. The assay uses a photometric measurement of the luciferin-luciferase reaction in erythrocytes incubated in HEPES buffer with the tested toxicant. Incubation in HEPES significantly increases the sensitivity of erythrocytes to beryllium when compared to incubation in either plasma or physiological saline. After a one-hour incubation period in HEPES buffer and beryllium there is a loss of 50% of the erythrocyte A TPat 3 \u03bcg\/ml of beryllium, and an 80% loss of ATP at 5 \u03bcg\/ml of beryllium. The source of human erythrocytes does not appear to influence the test. Erythrocytes from 10 individuals, one with chronic beryllium disease and another with an acute sensitivity to beryllium, all gave similar biphasic dose-response curves to beryllium.<\/jats:p>","DOI":"10.1177\/074823378800400101","type":"journal-article","created":{"date-parts":[[2013,9,9]],"date-time":"2013-09-09T18:31:23Z","timestamp":1378751483000},"page":"1-9","source":"Crossref","is-referenced-by-count":2,"title":["A Human Cell Beryllium Acute Toxicity Assay"],"prefix":"10.1177","volume":"4","author":[{"given":"S.M.","family":"Clarke","sequence":"first","affiliation":[{"name":"Rockwell International Corporation Golden, Colorado"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.W.","family":"Barrick","sequence":"additional","affiliation":[{"name":"Rockwell International Corporation Golden, Colorado"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[1988,1,1]]},"reference":[{"key":"atypb1","unstructured":"Berry, J.W., Osgood, D.W. and ST. John, P.A. (1974). Chemical Villains, p. 87. St. Louis: C.V. Mosby Company."},{"key":"atypb2","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(78)57003-1"},{"key":"atypb3","unstructured":"Harber, M.J. (1982). Applications of luminescence in medical microbiology and hematology. In: Clinical and Biochemical Luminescence (L.J. Kricka and T.J.N. Carter, eds.), p. 205. New York: Marcel Dekker, Inc."},{"key":"atypb4","volume-title":"Symposium Proceedings","author":"Love, G.J."},{"key":"atypb5","doi-asserted-by":"publisher","DOI":"10.1038\/194877a0"},{"key":"atypb6","volume-title":"Proceedings of the First International Symposium on Toxicity Testing Using Bacteria","author":"Qureshi, A.A."},{"key":"atypb7","doi-asserted-by":"publisher","DOI":"10.3109\/10408447209103366"},{"key":"atypb8","unstructured":"Vorwald, A.J. (1966). Medical aspects of beryllium disease. In: Beryllium: Its Industrial Hygiene Aspects ( H.E. Stokinger, ed.), pp. 167-200. New York: Academic Press."},{"key":"atypb9","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1002\/tox.2540020204","volume":"2","author":"Xu, H.","year":"1983","journal-title":"Toxicity Assessment"}],"container-title":["Toxicology and Industrial Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/074823378800400101","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/074823378800400101","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T20:46:07Z","timestamp":1777668367000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/074823378800400101"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1988,1]]},"references-count":9,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1988,1]]}},"alternative-id":["10.1177\/074823378800400101"],"URL":"https:\/\/doi.org\/10.1177\/074823378800400101","relation":{},"ISSN":["0748-2337","1477-0393"],"issn-type":[{"value":"0748-2337","type":"print"},{"value":"1477-0393","type":"electronic"}],"subject":[],"published":{"date-parts":[[1988,1]]}}}