{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T11:31:15Z","timestamp":1784892675187,"version":"3.55.0"},"reference-count":61,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2016,3,29]],"date-time":"2016-03-29T00:00:00Z","timestamp":1459209600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Biol Rhythms"],"published-print":{"date-parts":[[2016,6]]},"abstract":"<jats:p>The circadian rhythms found in almost all organisms are driven by molecular oscillators, including transcription\/translation feedback loops (TTFLs). However, TTFL-independent oscillators can drive rhythms in both eukaryotes and prokaryotes. The fungus Neurospora crassa is a model organism for studying the molecular mechanism of the circadian clock. Although a circadian TTFL involving the proteins FRQ, WC-1, and WC-2 is well-characterized in N. crassa, rhythms can still be observed in the absence of this feedback loop. These rhythms are said to be driven by 1 or more FRQ-less oscillator(s) (FLOs). The prd-1 mutation lengthens the period in frq wild type and was previously shown to severely disrupt FRQ-less rhythms in frq null mutants under several different conditions; therefore, the prd-1 gene product is a candidate for a component of a FLO. We report here that prd-1 also disrupts free-running rhythms in wc-1 null mutants, confirming its effects on FRQ-less rhythms. We have now mapped and identified the prd-1 gene as NCU07839, a DEAD-box RNA helicase dbp-2. Complementation with the wild-type gene corrects the rhythm defects of the prd-1 mutant in the complete circadian system (when the FRQ-based TTFL is intact) and also the free-running FRQ-less rhythm on low choline. A PRD-1\u2013GFP fusion protein localizes to the nucleus. The prd-1 mutant has a single base pair change in the first base of an intron that results in abnormally spliced transcripts. FRQ-less rhythms on low choline, or entrained to heat pulses, were only marginally affected in strains carrying deletions of 2 other RNA helicases ( prd-6 and msp-8). We conclude that PRD-1 is a member of an RNA helicase family that may be specifically involved in regulating rhythmicity in N. crassa in both the complete circadian system and FLO(s).<\/jats:p>","DOI":"10.1177\/0748730416639717","type":"journal-article","created":{"date-parts":[[2016,3,30]],"date-time":"2016-03-30T20:57:35Z","timestamp":1459371455000},"page":"258-271","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":18,"title":["PRD-1, a Component of the Circadian System of\n                    <i>Neurospora crassa<\/i>\n                    , Is a Member of the DEAD-box RNA Helicase Family"],"prefix":"10.1177","volume":"31","author":[{"given":"Keyur","family":"Adhvaryu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ghazaleh","family":"Firoozi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kamyar","family":"Motavaze","sequence":"additional","affiliation":[{"name":"Dalla Lana School of Public Health, University of Toronto, Toronto, Ontario M5T 1R4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"Lakin-Thomas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2016,3,29]]},"reference":[{"key":"bibr1-0748730416639717","doi-asserted-by":"publisher","DOI":"10.1021\/bi5005624"},{"key":"bibr2-0748730416639717","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1266805"},{"key":"bibr3-0748730416639717","doi-asserted-by":"publisher","DOI":"10.1177\/0748730404265532"},{"key":"bibr4-0748730416639717","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.383075"},{"key":"bibr5-0748730416639717","doi-asserted-by":"publisher","DOI":"10.1261\/rna.047985.114"},{"key":"bibr6-0748730416639717","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0601456103"},{"key":"bibr7-0748730416639717","unstructured":"Compton JE (2003) Advances in understanding the molecular biology of circadian rhythms in\n                      Neurospora crassa\n                      . 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