{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T05:39:58Z","timestamp":1777786798005,"version":"3.51.4"},"reference-count":11,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2010,3,17]],"date-time":"2010-03-17T00:00:00Z","timestamp":1268784000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Biomater Appl"],"published-print":{"date-parts":[[2011,7]]},"abstract":"<jats:p>The introduction of a fluorescent chromaphore into bifunctional crosslinkers results in a molecule with normal crosslinker properties and a fluorescent group for straightforward quantification. This work describes the synthesis of the dansyl-labeled heterobifunctional crosslinker N-succinimidyl \u03b5-N-dansyl \u03b1-N-(acetylthio)acetyllysine (dansyl-ATA-lysine-NHS) containing reactive N-hydroxysuccinimidyl (NHS) ester and sulfhydryl groups. The application of this crosslinker to conjugation of bovine serum albumin (BSA) protein to the surface of a liposome containing maleimide functions is also demonstrated. BSA was modified with the dansyl-labeled crosslinker and subsequently conjugated to liposomes containing reactive phospholipid derivative N-[4-(p-maleimidophenyl)butyryl]phosphatidylethanolamine and the degree of modification and conjugation were quantitatively determined by measuring the fluorescence emission of the dansyl group. The reliability of the fluorescence quantification was confirmed by a micro bio-barcode assay protein assay.<\/jats:p>","DOI":"10.1177\/0885328210363503","type":"journal-article","created":{"date-parts":[[2010,3,17]],"date-time":"2010-03-17T21:52:38Z","timestamp":1268862758000},"page":"117-125","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["Modification of Liposomes with Proteins by Dansyl-labeled Heterobifunctional Crosslinker"],"prefix":"10.1177","volume":"26","author":[{"given":"Tao","family":"Chen","sequence":"first","affiliation":[{"name":"Faculty of Life Sciences, Northwestern Polytechnical University Xi'an, Shaanxi 710072, PR China, Shaanxi Liposome Research Center, Xi'an, Shaanxi 710075, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rutao","family":"Wang","sequence":"additional","affiliation":[{"name":"Faculty of Life Sciences, Northwestern Polytechnical University Xi'an, Shaanxi 710072, PR China; Shaanxi Liposome Research Center, Xi'an, Shaanxi 710075, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tingting","family":"Lu","sequence":"additional","affiliation":[{"name":"Faculty of Sciences, Northwestern Polytechnical University Xi'an, Shaanxi 710072, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guozheng","family":"Liang","sequence":"additional","affiliation":[{"name":"Faculty of Sciences, Northwestern Polytechnical University Xi'an, Shaanxi 710072, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tingli","family":"Lu","sequence":"additional","affiliation":[{"name":"Faculty of Life Sciences, Northwestern Polytechnical University Xi'an, Shaanxi 710072, PR China; Shaanxi Liposome Research Center, Xi'an, Shaanxi 710075, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2010,3,17]]},"reference":[{"key":"atypb1","doi-asserted-by":"publisher","DOI":"10.2147\/nano.2006.1.1.97"},{"key":"atypb2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00986726"},{"key":"atypb3","first-page":"2","volume":"3","author":"Brinkley, M.","year":"1992","journal-title":"Chem."},{"key":"atypb4","doi-asserted-by":"publisher","DOI":"10.1016\/0141-0229(92)90049-T"},{"key":"atypb5","doi-asserted-by":"publisher","DOI":"10.1021\/bc00001a001"},{"key":"atypb6","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/S0165-0270(96)00138-0","volume":"71","author":"Vanessa, H.R.","year":"1997","journal-title":"J. Neurosci. Meth."},{"key":"atypb7","doi-asserted-by":"publisher","DOI":"10.1021\/jo0709293"},{"key":"atypb8","doi-asserted-by":"publisher","DOI":"10.1042\/bj1730723"},{"key":"atypb9","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2003.08.041"},{"key":"atypb10","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(83)90426-8"},{"key":"atypb11","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2736(85)90521-8"}],"container-title":["Journal of Biomaterials Applications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0885328210363503","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0885328210363503","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T19:48:16Z","timestamp":1777492096000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/0885328210363503"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,3,17]]},"references-count":11,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2011,7]]}},"alternative-id":["10.1177\/0885328210363503"],"URL":"https:\/\/doi.org\/10.1177\/0885328210363503","relation":{},"ISSN":["0885-3282","1530-8022"],"issn-type":[{"value":"0885-3282","type":"print"},{"value":"1530-8022","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,3,17]]}}}