{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T06:12:41Z","timestamp":1777788761535,"version":"3.51.4"},"reference-count":49,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2017,7,27]],"date-time":"2017-07-27T00:00:00Z","timestamp":1501113600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Biomater Appl"],"published-print":{"date-parts":[[2017,9]]},"abstract":"<jats:p>To restore damaged organ function or to investigate organ mechanisms, it is necessary to prepare replicates that follow the biological role model as faithfully as possible. The interdisciplinary field of tissue engineering has great potential in regenerative medicine and might overcome negative side effects in the replacement of damaged organs. In particular, tubular organ structures of the genitourinary tract, such as the ureter and urethra, are challenging because of their complexity and special milieu that gives rise to incrustation, inflammation and stricture formation. Tubular biohybrids were prepared from primary porcine smooth muscle cells embedded in a fibrin gel with a stabilising poly(vinylidene fluoride) mesh. A mechanotransduction was performed automatically with a balloon kyphoplasty catheter. Diffusion of urea and creatinine, as well as the bursting pressure, were measured. Light and electron microscopy were used to visualise cellular distribution and orientation. Histological evaluation revealed a uniform cellular distribution in the fibrin gel. Mechanical stimulation with a stretch of 20% leads to a circumferential orientation of smooth muscle cells inside the matrix and a longitudinal alignment on the outer surface of the tubular structure. Urea and creatinine permeability and bursting pressure showed a non-statistically significant trend towards stimulated tissue constructs. In this proof of concept study, an innovative technique of intraluminal pressure for mechanical stimulation of tubular biohybrids prepared from autologous cells and a composite material induce bi-directional orientation of smooth muscle cells by locally and cyclically applied mechanical tension. Such geometrically driven patterns of cell growth within a scaffold may represent a key stage in the future tissue engineering of implantable ureter replacements that will allow the active transportation of urine from the renal pelvis into the bladder.<\/jats:p>","DOI":"10.1177\/0885328217723178","type":"journal-article","created":{"date-parts":[[2017,7,28]],"date-time":"2017-07-28T02:04:38Z","timestamp":1501207478000},"page":"321-330","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":12,"title":["Mechanical induction of bi-directional orientation of primary porcine bladder smooth muscle cells in tubular fibrin-poly(vinylidene fluoride) scaffolds for ureteral and urethral repair using cyclic and focal balloon catheter stimulation"],"prefix":"10.1177","volume":"32","author":[{"given":"Volker","family":"Seifarth","sequence":"first","affiliation":[{"name":"Institute for Bioengineering (IfB), Laboratory of Medical and Molecular Biology, FH Aachen, Aachen, Germany"},{"name":"Department of Urology, RWTH Aachen University Hospital, Aachen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joachim O","family":"Grosse","sequence":"additional","affiliation":[{"name":"Department of Urology, RWTH Aachen University Hospital, Aachen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthias","family":"Gossmann","sequence":"additional","affiliation":[{"name":"Institute for Bioengineering (IfB), Laboratory of Medical and Molecular Biology, FH Aachen, Aachen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heinz Peter","family":"Janke","sequence":"additional","affiliation":[{"name":"Department of Urology, Radboud Institute for Molecular Life Science, Radboud University Medical Center, Nijmegen, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick","family":"Arndt","sequence":"additional","affiliation":[{"name":"Department of Urology, RWTH Aachen University Hospital, Aachen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sabine","family":"Koch","sequence":"additional","affiliation":[{"name":"AME-Helmholtz Institute for Biomedical Engineering, Biohybrid &amp; Medical Textiles (BioTex), RWTH Aachen University, Aachen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthias","family":"Epple","sequence":"additional","affiliation":[{"name":"Department for Inorganic Chemistry and Center for Nanointegration Duisburg-Essen (CeNIDE), University of Duisburg-Essen, Essen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gerhard M","family":"Artmann","sequence":"additional","affiliation":[{"name":"Institute for Bioengineering (IfB), Laboratories of Cell Biophysics, FH Aachen, Campus J\u00fclich, J\u00fclich, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ayseg\u00fcl Temiz","family":"Artmann","sequence":"additional","affiliation":[{"name":"Institute for Bioengineering (IfB), Laboratories of Medical and Molecular Biology, FH Aachen, Campus J\u00fclich, J\u00fclich, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2017,7,27]]},"reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmjopen-2013-004711"},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.1136\/jech-2014-204083"},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.1089\/end.1999.13.241"},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.1089\/089277902753483682"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.ju.0000121437.94629.ef"},{"key":"e_1_3_5_7_2","doi-asserted-by":"publisher","DOI":"10.1590\/S1677-55382004000400013"},{"key":"e_1_3_5_8_2","doi-asserted-by":"publisher","DOI":"10.1089\/end.2005.19.1069"},{"key":"e_1_3_5_9_2","doi-asserted-by":"publisher","DOI":"10.1089\/0892779041271472"},{"key":"e_1_3_5_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2011.09.055"},{"key":"e_1_3_5_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(05)64414-X"},{"key":"e_1_3_5_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2012.07.041"},{"key":"e_1_3_5_13_2","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2013.0185"},{"key":"e_1_3_5_14_2","first-page":"5","article-title":"Engineering biological-based vascular grafts using a pulsatile bioreactor","volume":"1","author":"Huang AH","year":"2011","unstructured":"Huang AH, Niklason LE. Engineering biological-based vascular grafts using a pulsatile bioreactor. J Vis Exp 2011; 1: 5\u201310.","journal-title":"J Vis Exp"},{"key":"e_1_3_5_15_2","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2015.0048"},{"key":"e_1_3_5_16_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bioeng.9.060906.151927"},{"key":"e_1_3_5_17_2","volume-title":"Experimental analysis of biaxial mechanical tension in cell monolayers and cultured three-dimensional tissues \u2013 The CellDrum Technology","author":"Trzewik J","year":"2007","unstructured":"Trzewik J. Experimental analysis of biaxial mechanical tension in cell monolayers and cultured three-dimensional tissues \u2013 The CellDrum Technology, University library Ilmenau urn:nbn:de:gbv:ilm1-2007000305: Technical University Ilmenau, 2007."},{"key":"e_1_3_5_18_2","doi-asserted-by":"publisher","DOI":"10.1114\/B:ABME.0000039358.71180.9a"},{"key":"e_1_3_5_19_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12195-010-0149-4"},{"key":"e_1_3_5_20_2","first-page":"56","article-title":"Ureplace: development of a bioreactor for in\u00a0vitro culturing of cell seeded tubular vessels on collagen scaffolds","volume":"2","author":"Seifarth V","year":"2011","unstructured":"Seifarth V, Schehl D, Linder P, et al. Ureplace: development of a bioreactor for in\u00a0vitro culturing of cell seeded tubular vessels on collagen scaffolds. Al-Farabi Kazakh Natl Univ Bull Biol Ser 2011; 2: 56\u201358.","journal-title":"Al-Farabi Kazakh Natl Univ Bull Biol Ser"},{"key":"e_1_3_5_21_2","doi-asserted-by":"publisher","DOI":"10.1089\/tea.2006.0398"},{"key":"e_1_3_5_22_2","first-page":"568","article-title":"A novel bioreactor to simulate urinary bladder mechanical","volume":"124","author":"Xin W","year":"2011","unstructured":"Xin W, Dao-bing L, Feng X, et al. A novel bioreactor to simulate urinary bladder mechanical. Chin Med J (Engl) 2011; 124: 568\u2013573.","journal-title":"Chin Med J (Engl)"},{"key":"e_1_3_5_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2011.06.036"},{"key":"e_1_3_5_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2004.04.036"},{"key":"e_1_3_5_25_2","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2012.0738"},{"key":"e_1_3_5_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2013.09.067"},{"key":"e_1_3_5_27_2","volume-title":"Ureteral tissue engineering: development of a bioreactor system and subsequent characterization of the generated biohybrids","author":"Seifarth V","year":"2015","unstructured":"Seifarth V. Ureteral tissue engineering: development of a bioreactor system and subsequent characterization of the generated biohybrids, DuEPublico: University Duisburg-Essen, 2015."},{"key":"e_1_3_5_28_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2010.09680.x"},{"key":"e_1_3_5_29_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-016-1112-5"},{"key":"e_1_3_5_30_2","doi-asserted-by":"publisher","DOI":"10.1177\/0885328215610824"},{"key":"e_1_3_5_31_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10439-011-0437-8"},{"key":"e_1_3_5_32_2","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-134-34792"},{"key":"e_1_3_5_33_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041380103"},{"key":"e_1_3_5_34_2","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2009.0235"},{"key":"e_1_3_5_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2006.08.027"},{"key":"e_1_3_5_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1010-7940(00)00373-0"},{"key":"e_1_3_5_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1010-7940(01)00624-8"},{"key":"e_1_3_5_38_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1525-1594.2008.00601.x"},{"key":"e_1_3_5_39_2","doi-asserted-by":"publisher","DOI":"10.1002\/bies.201200062"},{"key":"e_1_3_5_40_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.34340"},{"key":"e_1_3_5_41_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bioeng.3.1.225"},{"key":"e_1_3_5_42_2","doi-asserted-by":"publisher","DOI":"10.1114\/1.275"},{"key":"e_1_3_5_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.anuro.2005.01.002"},{"key":"e_1_3_5_44_2","doi-asserted-by":"publisher","DOI":"10.1159\/000368419"},{"key":"e_1_3_5_45_2","doi-asserted-by":"crossref","unstructured":"Jockenhoevel S and Flanagan TC. Cardiovascular tissue engineering based on fibrin-gel-scaffolds tissue engineering for tissue and organ regeneration. In: Prof. Eberli D (ed.) InTech https:\/\/www.intechopen.com\/books\/tissue-engineering-for-tissue-and-organ-regeneration\/cardiovascular-tissue-engineering-based-on-fibrin-gel-scaffolds (2011). DOI: 10.5772\/19761.","DOI":"10.5772\/19761"},{"key":"e_1_3_5_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2012.03.119"},{"key":"e_1_3_5_47_2","doi-asserted-by":"publisher","DOI":"10.1002\/bit.22190"},{"key":"e_1_3_5_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2011.05.051"},{"key":"e_1_3_5_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2009.09.086"},{"key":"e_1_3_5_50_2","first-page":"686","article-title":"Mechanical stress induced cellular orientation and phenotypic modulation of 3-D cultured smooth muscle cells","volume":"39","author":"Kanda K","year":"1993","unstructured":"Kanda K, Matsuda T, Oka T. Mechanical stress induced cellular orientation and phenotypic modulation of 3-D cultured smooth muscle cells. ASAIO J 1993; 39: 686\u2013690.","journal-title":"ASAIO J"}],"container-title":["Journal of Biomaterials Applications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0885328217723178","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/0885328217723178","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0885328217723178","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T19:54:25Z","timestamp":1777492465000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/0885328217723178"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,7,27]]},"references-count":49,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2017,9]]}},"alternative-id":["10.1177\/0885328217723178"],"URL":"https:\/\/doi.org\/10.1177\/0885328217723178","relation":{},"ISSN":["0885-3282","1530-8022"],"issn-type":[{"value":"0885-3282","type":"print"},{"value":"1530-8022","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,7,27]]}}}