{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T05:36:04Z","timestamp":1777786564101,"version":"3.51.4"},"reference-count":56,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2017,10,1]],"date-time":"2017-10-01T00:00:00Z","timestamp":1506816000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Biomater Appl"],"published-print":{"date-parts":[[2017,10]]},"abstract":"<jats:p>\n                    Recombinant human bone morphogenetic protein (BMP)-2 is an FDA-approved therapy for nonunion tibia fracture, though it has a number of biological and practical disadvantages. Our research group has developed a novel tissue engineering strategy termed antibody-mediated osseous regeneration. This entails application of anti-BMP-2 monoclonal antibodies (mAbs) to capture endogenous BMP\u2019s to mediate in\u00a0vivo bone formation. This has been documented in a number of animal models. The present exploratory study sought to investigate the application of antibody-mediated osseous regeneration for repair of nonunion tibia defect in a nonhuman primate model. A 20\u2009mm segmental osteotomy was performed in tibia of 6 Macaca fascicularis and was implanted with absorbable collagen sponge that was functionalized with chimeric anti-BMP-2 or isotype matched control mAb. Cone beam computed tomography (CBCT), histologic and histomorphometric analyses were performed 12 weeks post-operatively. CBCT analyzed by quantitative 3D volumetric analysis revealed that sites implanted with absorbable collagen sponge functionalized with anti-BMP-2\u2009mAb demonstrated numerically higher mineralized tissue (408\u2009\u00b1\u2009127\u2009mm\n                    <jats:sup>3<\/jats:sup>\n                    ) compared with sites implanted with isotype matched control mAb (214\u2009\u00b1\u200981\u2009mm\n                    <jats:sup>3<\/jats:sup>\n                    ), though the difference was not statistically significant ( p\u2009=\u20090.09). Histologic and histomorphometric analysis showed de novo bone formation with greater ( p\u2009&lt;\u20090.01) percentage of bone volume in sites implanted with anti-BMP-2 (41.3\u2009\u00b1\u20094.4%), compared with isotype matched control mAb (14.6\u2009\u00b1\u20095.6%). Results from the present exploratory study provide evidence for the potential of anti-BMP-2\u2009mAb to mediate repair of a large segmental tibia defects in a nonhuman primate model. Therapeutic antibodies have generally been shown to have great safety and efficacy profile, though their application in tissue engineering has been limited in the past. Following further investigation, anti-BMP-2\u2009mAbs immobilized on appropriate scaffold may have application in repair of large skeletal defects without the need for exogenous growth factors.\n                  <\/jats:p>","DOI":"10.1177\/0885328217733262","type":"journal-article","created":{"date-parts":[[2017,10,10]],"date-time":"2017-10-10T02:38:46Z","timestamp":1507603126000},"page":"425-432","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":5,"title":["Collagen sponge functionalized with chimeric anti-BMP-2 monoclonal antibody mediates repair of nonunion tibia defects in a nonhuman primate model: An exploratory study"],"prefix":"10.1177","volume":"32","author":[{"given":"Lijia","family":"Guo","sequence":"first","affiliation":[{"name":"Laboratory of Tissue Regeneration and Immunology and Department of Periodontics, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Capital Medical University School of Stomatology, Beijing, China"},{"name":"Department of Orthodontics, Capital Medical University School of Stomatology, Beijing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0716-0616","authenticated-orcid":false,"given":"Seiko","family":"Min","sequence":"additional","affiliation":[{"name":"Laboratory for Immunoregulation and Tissue Engineering (LITE), Ostrow School of Dentistry, University of Southern California, Los Angeles, CA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yingying","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Stomatology, Beijing Tiantan Hospital, Capital Medical University School of Stomatology, Beijing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianxia","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery, Xiangya Stomatological Hospital, Central South University, Changsha, Hunan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan","family":"Du","sequence":"additional","affiliation":[{"name":"Laboratory of Tissue Regeneration and Immunology and Department of Periodontics, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Capital Medical University School of Stomatology, Beijing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bee Tin","family":"Goh","sequence":"additional","affiliation":[{"name":"Department of Oral & Maxillofacial Surgery, National Dental Centre, Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leonardo","family":"Saigo","sequence":"additional","affiliation":[{"name":"Department of Oral & Maxillofacial Surgery, National Dental Centre, Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Songlin","family":"Wang","sequence":"additional","affiliation":[{"name":"Molecular Laboratory for Gene Therapy and 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