{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T20:14:02Z","timestamp":1778184842790,"version":"3.51.4"},"reference-count":57,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2021,6,23]],"date-time":"2021-06-23T00:00:00Z","timestamp":1624406400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Biomater Appl"],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:p>In this study, it was aimed to present an approach for the development of multi-layered tissue engineering constructs by using cell sheet engineering. Briefly, MC3T3-E1 mouse pre-osteoblast cells were cultured in temperature-responsive plates (Nunc Upcell\u00ae) in the presence of osteogenic medium and the resulting cell sheets were laminated with electrospun poly(L-lactic acid) (PLLA) membranes to obtain viable three-dimensional, thick constructs. The constructs prepared without PLLA membranes were used as control. The cell viability and death in the resulting structures were investigated by microscopic and colorimetric methods. The in vitro performance of the structures was discussed comparatively. Alkaline phosphatase (ALP) activity, collagen and sulfated glycosaminoglycan (sGAG) content values were calculated. The presented approach shows potential for engineering applications of complex tissues with at least two or more microenvironments such as osteochondral, corneal or vascular tissues.<\/jats:p>","DOI":"10.1177\/08853282211027889","type":"journal-article","created":{"date-parts":[[2021,6,24]],"date-time":"2021-06-24T02:34:30Z","timestamp":1624502070000},"page":"818-833","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":10,"title":["Multi-layered\n                    <i>in vitro<\/i>\n                    3D-bone model via combination of osteogenic cell sheets with electrospun membrane interlayer"],"prefix":"10.1177","volume":"36","author":[{"given":"Atakan","family":"Tevlek","sequence":"first","affiliation":[{"name":"Institute of Science, Hacettepe University, Ankara, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0732-1785","authenticated-orcid":false,"given":"Halil Murat","family":"Aydin","sequence":"additional","affiliation":[{"name":"Institute of Science, Hacettepe University, Ankara, Turkey"},{"name":"Centre for Bioengineering, Hacettepe University, Ankara, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2021,6,23]]},"reference":[{"key":"bibr1-08853282211027889","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-med-102715-092331"},{"key":"bibr2-08853282211027889","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.201601118"},{"key":"bibr3-08853282211027889","doi-asserted-by":"publisher","DOI":"10.1080\/09205063.2015.1059018"},{"key":"bibr4-08853282211027889","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsusc.2020.145572"},{"key":"bibr5-08853282211027889","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201907225"},{"key":"bibr6-08853282211027889","doi-asserted-by":"crossref","unstructured":"Benchaprathanphorn K, Sakulaue P, Siriwatwechakul W, et\u00a0al. Preparation and characterization of human keratinocyte\u2013fibroblast cell sheets constructed using PNIAM-co-AM grafted surfaces for burn wound healing.\n                      J Mater Sci Mater Med.\n                      2020; 31: 126.","DOI":"10.1007\/s10856-020-06469-4"},{"key":"bibr7-08853282211027889","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2014.05.024"},{"key":"bibr8-08853282211027889","doi-asserted-by":"publisher","DOI":"10.1002\/biot.201300432"},{"key":"bibr9-08853282211027889","doi-asserted-by":"publisher","DOI":"10.1007\/s12272-013-0299-8"},{"key":"bibr10-08853282211027889","doi-asserted-by":"crossref","unstructured":"Sakaguchi K, Takahashi H, Tobe Y, et\u00a0al. Measuring the contractile force of multilayered human cardiac cell sheets.\n                      Tissue Eng \u2013 Part C Methods\n                      2020; 26: 485.","DOI":"10.1089\/ten.tec.2020.0164"},{"key":"bibr11-08853282211027889","doi-asserted-by":"crossref","unstructured":"Rezapour-Lactoee A, Yeganeh H, Gharibi R, et\u00a0al. Enhanced healing of a full-thickness wound by a thermoresponsive dressing utilized for simultaneous transfer and protection of adipose-derived mesenchymal stem cells sheet.\n                      J Mater Sci Mater Med.\n                      2020; 31: 101.","DOI":"10.1007\/s10856-020-06433-2"},{"key":"bibr12-08853282211027889","doi-asserted-by":"publisher","DOI":"10.3390\/bioengineering8010007"},{"key":"bibr13-08853282211027889","doi-asserted-by":"crossref","unstructured":"Shamsah AH, Cartmell SH, Richardson SM, et\u00a0al. Tissue engineering the annulus fibrosus using 3D rings of electrospun PCL:PLLA angle-ply nanofiber sheets.\n                      Front Bioeng Biotechnol.\n                      2020; 7: 437.","DOI":"10.3389\/fbioe.2019.00437"},{"key":"bibr14-08853282211027889","doi-asserted-by":"crossref","unstructured":"Nakamura K, Saotome T, Shimada N, et\u00a0al. A gelatin hydrogel nonwoven fabric facilitates metabolic activity of multilayered cell sheets.\n                      Tissue Eng \u2013 Part C Methods\n                      2019; 25: 344.","DOI":"10.1089\/ten.tec.2019.0061"},{"key":"bibr15-08853282211027889","doi-asserted-by":"crossref","unstructured":"Song SY, Kim H, Yoo J, et\u00a0al. Prevascularized, multiple-layered cell sheets of direct cardiac reprogrammed cells for cardiac repair.\n                      Biomater Sci\n                      2020; 8: 4508.","DOI":"10.1039\/D0BM00701C"},{"key":"bibr16-08853282211027889","doi-asserted-by":"publisher","DOI":"10.1177\/0885328219862123"},{"key":"bibr17-08853282211027889","doi-asserted-by":"publisher","DOI":"10.1177\/0963689718809918"},{"key":"bibr18-08853282211027889","doi-asserted-by":"publisher","DOI":"10.1096\/fj.05-4715fje"},{"key":"bibr19-08853282211027889","doi-asserted-by":"crossref","unstructured":"Vaquette C, Sudheesh Kumar PT, Petcu EB, et\u00a0al. Combining electrospinning and cell sheet technology for the development of a multiscale tissue engineered ligament construct (TELC).\n                      J Biomed Mater Res \u2013 Part B Appl Biomater\n                      2018; 106: 399.","DOI":"10.1002\/jbm.b.33828"},{"key":"bibr20-08853282211027889","doi-asserted-by":"publisher","DOI":"10.1002\/term.1765"},{"key":"bibr21-08853282211027889","doi-asserted-by":"publisher","DOI":"10.1088\/1758-5090\/aa64a0"},{"key":"bibr22-08853282211027889","doi-asserted-by":"crossref","unstructured":"Sekine W, Haraguchi Y, Shimizu T, et\u00a0al. Thickness limitation and cell viability of multi-layered cell sheets and overcoming the diffusion limit by a porous-membrane culture insert.\n                      Biochips Tissue Chips\n                      2011; S1: 007.","DOI":"10.4172\/2153-0777.S1-007"},{"key":"bibr23-08853282211027889","doi-asserted-by":"crossref","unstructured":"Sathy BN, Mony U, Menon D, et\u00a0al. Bone tissue engineering with multilayered scaffolds - Part I: an approach for vascularizing engineered constructs in vivo.\n                      Tissue Eng-Part A\n                      2015; 21: 2480.","DOI":"10.1089\/ten.tea.2015.0098"},{"key":"bibr24-08853282211027889","unstructured":"Fattahi F-S, Khoddami A, Avinc O. Poly(lactic acid) (PLA) nanofibers for bone tissue engineering.\n                      J. Text Polym\n                      2019; 7: 47."},{"key":"bibr25-08853282211027889","unstructured":"ISO 10993-5:2009 Biological Evaluation of Medical Devices-Part 5: Tests for\n                      in vitro\n                      cytotoxicity, 2009."},{"key":"bibr26-08853282211027889","doi-asserted-by":"crossref","unstructured":"Tevlek A, Odabas S, Celik E, et\u00a0al. 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The extracellular matrix at a glance.\n                      J Cell Sci\n                      2010; 123: 4195.","DOI":"10.1242\/jcs.023820"},{"key":"bibr55-08853282211027889","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2020.00757"},{"key":"bibr56-08853282211027889","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/421746"},{"key":"bibr57-08853282211027889","doi-asserted-by":"publisher","DOI":"10.1177\/0885328217731951"}],"container-title":["Journal of Biomaterials Applications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/08853282211027889","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/08853282211027889","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/08853282211027889","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T19:51:35Z","timestamp":1777492295000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/08853282211027889"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6,23]]},"references-count":57,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2021,11]]}},"alternative-id":["10.1177\/08853282211027889"],"URL":"https:\/\/doi.org\/10.1177\/08853282211027889","relation":{},"ISSN":["0885-3282","1530-8022"],"issn-type":[{"value":"0885-3282","type":"print"},{"value":"1530-8022","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,6,23]]}}}