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In this study, we developed a multifunctional chitosan\/gelatin\/polyvinyl alcohol (CS\/GEL\/PVA) nanocomposite containing magnesium oxide (MgO) nanoparticles loaded with quercetin (MgO@QC), aimed at enhancing wound healing and promoting keratinocyte growth factor 1 (KGF1) expression. MgO nanoparticles were synthesized and characterized using DLS, zeta potential, FTIR, XRD, FESEM, and TEM. Quercetin was successfully loaded onto the MgO nanoparticles with a high loading efficiency of 99%, as confirmed by spectroscopic analyses. The resulting nanocomposite demonstrated favorable physicochemical properties, including uniform morphology, excellent swelling behavior (\u223c79%), optical clarity, and robust structural integrity. Hemolysis assays revealed excellent hemocompatibility, while in vitro cytotoxicity tests confirmed biocompatibility up to 500\u00a0\u00b5g\/mL. Cell proliferation and migration assays (MTT and scratch test) showed dose-dependent enhancement of fibroblast activity, particularly at 1\u00a0mg\/mL. The nanocomposite also significantly upregulated KGF1 gene expression, suggesting its role in stimulating epithelial regeneration. In vivo studies using a murine excisional wound model demonstrated accelerated wound closure and tissue regeneration in the MgO@QC-treated group, supported by histological evidence of angiogenesis, re-epithelialization, and reduced inflammation. The CS\/GEL\/PVA\/MgO@QC nanocomposite offers a biocompatible and bioactive platform that significantly enhances wound healing. These findings suggest its strong potential for clinical application as an advanced wound dressing for acute and chronic skin injuries.<\/jats:p>","DOI":"10.1177\/08853282251399589","type":"journal-article","created":{"date-parts":[[2025,11,17]],"date-time":"2025-11-17T23:57:25Z","timestamp":1763423845000},"page":"1332-1348","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["Quercetin-loaded MgO nanoparticles in a chitosan\/gelatin\/PVA matrix enhance KGF1 expression and accelerate wound healing"],"prefix":"10.1177","volume":"40","author":[{"given":"Najmeh","family":"Darvishi","sequence":"first","affiliation":[{"name":"Shahrekord University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6843-7645","authenticated-orcid":false,"given":"Somayeh","family":"Reiisi","sequence":"additional","affiliation":[{"name":"Shahrekord University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sadegh","family":"Shirian","sequence":"additional","affiliation":[{"name":"Shahrekord University"},{"name":"Dr Daneshbod Lab"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2025,11,17]]},"reference":[{"key":"e_1_3_7_2_2","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2018.00086"},{"key":"e_1_3_7_3_2","doi-asserted-by":"publisher","DOI":"10.5958\/0974-360X.2019.00240.3"},{"key":"e_1_3_7_4_2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00067.2017"},{"issue":"1","key":"e_1_3_7_5_2","first-page":"2684108","article-title":"Wound healing and the use of medicinal plants","volume":"2019","author":"Shedoeva A","year":"2019","unstructured":"Shedoeva A, Leavesley D, Upton Z, et al. 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