{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T03:55:23Z","timestamp":1785297323643,"version":"3.55.0"},"reference-count":57,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T00:00:00Z","timestamp":1769731200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100021803","name":"Central Institute of Fisheries Technology, Indian Council of Agricultural Research","doi-asserted-by":"publisher","award":["1000661052|P-115\/2020(3)|"],"award-info":[{"award-number":["1000661052|P-115\/2020(3)|"]}],"id":[{"id":"10.13039\/501100021803","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Biomater Appl"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:p>\n                    <jats:bold>Introduction:<\/jats:bold>\n                    Non-healing diabetic foot ulcers often result in amputation and reduced quality of life. The study objective was to develop and evaluate a novel fish skin-derived acellular dermal matrix (FSADM) for diabetic wound-healing applications, addressing these challenges.\n                    <jats:bold>Methods:<\/jats:bold>\n                    FSADM was fabricated from yellow fin tuna (\n                    <jats:italic toggle=\"yes\">Thunnus albacares<\/jats:italic>\n                    ) skin using a decellularization and lyophilization process. The matrix was characterized by Fourier-transform infrared spectroscopy (FTIR).\n                    <jats:italic toggle=\"yes\">In vitro<\/jats:italic>\n                    biocompatibility was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays with L929 fibroblasts and hemocompatibility tests.\n                    <jats:italic toggle=\"yes\">In vivo<\/jats:italic>\n                    , biocompatibility was evaluated through subcutaneous implantation in Sprague-Dawley rats. Wound-healing efficacy was assessed in a streptozotocin-induced diabetic rat model with full-thickness excisional wounds, comparing FSADM with commercial alternatives and untreated controls.\n                    <jats:bold>Results:<\/jats:bold>\n                    FTIR analysis confirmed the preservation of collagen structure in FSADM.\n                    <jats:italic toggle=\"yes\">In vitro<\/jats:italic>\n                    studies demonstrated cytocompatibility with L929 cells and minimal haemolytic activity (0.68 \u00b1 0.034%). Subcutaneous implantation demonstrated good biocompatibility, with a progressive reduction in the inflammatory response. In the diabetic wound model, FSADM-treated wounds exhibited significantly faster closure rates than commercial controls (\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    &lt; 0.05), achieving 100% closure by day 21, compared to 90% closure in commercial controls. Histological analysis revealed enhanced epithelialization, hair follicle formation, and angiogenesis in FSADM-treated wounds.\n                    <jats:bold>Conclusion:<\/jats:bold>\n                    FSADM demonstrates excellent biocompatibility and superior wound-healing in diabetic conditions compared to commercial alternatives. It presents a promising, sustainable biomaterial for diabetic wound care. Further studies are needed to validate these findings in clinical settings and optimize their therapeutic potential.\n                  <\/jats:p>","DOI":"10.1177\/08853282261422121","type":"journal-article","created":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T22:47:00Z","timestamp":1769813220000},"page":"221-232","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Development and evaluation of a fish skin-derived acellular dermal matrix for diabetic wound healing:\n                    <i>In vitro<\/i>\n                    and\n                    <i>in vivo<\/i>\n                    assessment"],"prefix":"10.1177","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1593-2479","authenticated-orcid":false,"given":"Mekha Grace","family":"Varghese","sequence":"first","affiliation":[{"name":"Stem cell and tissue engineering lab, Pushpagiri Research Center, Pushpagiri Institute of Medical 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