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In this study, nanoparticles are designed in such a way that, the nanoparticle does gradient temporal delivery for chemotaxis followed by residual insulin in the nanoparticles for spatial presentation leading to a regenerative nanoparticle of insulin as a growth factor substitute. FTIR and NMR analyses supported the association of stearate with albumin. The optimized nanoparticles had an average diameter of 210\u00a0nm by DLS and a zeta potential of \u221235.6\u00a0mV. SEM and HRTEM analyses showed nanoscale and submicroscopic structures with elongated and higher-order morphologies, wherein insulin loading is associated with changes in particle organization. The BGFS showed sustained insulin release for up to 240 hours, with approximately 40% of the insulin released during this period, while the remaining insulin was retained within the matrix. The nanoparticles showed low hemolysis (&lt;5%), high cellular viability (74\u201394%), and physical stability under refrigerated conditions. In a full-thickness wound model in rats, the formulation is associated with improved wound healing response from histology, including collagen deposition and neovascularization, without detectable hypoglycemia. 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