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Tai Chi is believed to have a positive impact on these disturbances. This study examined the network structures of depression and anxiety among older Tai Chi practitioners vs non-practitioners. Propensity score matching (PSM) was used to construct a non-practitioner group based on Chinese Longitudinal Healthy Longevity Survey (CLHLS) data. 346 Tai Chi practitioners and 1019 non-practitioners were included. The prevalence and severity rates of depression were significantly lower among Tai Chi practitioners compared to non-practitioners though there was no group difference for anxiety. Network analysis showed that, among Tai Chi practitioners, \u201cFelt sadness\u201d, \u201cUncontrollable worrying\u201d and \u201cTrouble relaxing\u201d were the most central symptoms. In contrast, among non-practitioners, the most central symptoms were \u201cFelt sadness\u201d, \u201cUncontrollable worrying\u201d, and \u201cNervousness\u201d. Key bridge symptoms linking depressive and anxiety symptoms were \u201cWorry too much\u201d, \u201cBothered by things\u201d and \u201cUncontrollable worrying\u201d in Tai Chi practitioners and \u201cNervousness\u201d, \u201cFelt nervous\/fearful\u201d and \u201cSleep quality\u201d in non-practitioners. This study underscored how practising Tai Chi is associated with reduced overall prevalence and severity of depression and different interactions of depressive and anxiety symptoms among older adults. 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