{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,13]],"date-time":"2026-09-13T08:04:41Z","timestamp":1789286681230,"version":"build-2803163510"},"reference-count":42,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T00:00:00Z","timestamp":1788998400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"Inner Mongolia Medical University Higher Education Teaching Reform Research Project","award":["NYJXGG SJ2025025"],"award-info":[{"award-number":["NYJXGG SJ2025025"]}]},{"name":"2024 Science and Technology Project for the Development of High-Level Clinical Specialities in Public Hospitals in the Capital Region","award":["2024SGGZ077"],"award-info":[{"award-number":["2024SGGZ077"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Geriatr Psychiatry Neurol"],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Post-stroke cognitive impairment (PSCI) plays an important role in recovery and quality of life in elderly stroke patients. MicroRNAs play critical roles in the pathophysiology of PSCI.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>The expression of miR-378a-3p in elderly stroke patients and its potential as a predictive biomarker for PSCI was explored.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Serum samples from elderly stroke patients were collected for RT-qPCR analysis to evaluate miR-378a-3p. The Montreal Cognitive Assessment (MoCA) was performed to assess cognitive function. An oxygen-glucose deprivation (OGD) model with HT22 neuronal cells was employed to simulate ischemic injury in vitro. Functional assays, including dual-luciferase reporter assays, evaluated the direct targeting of TLR8 by miR-378a-3p. Cell viability was measured after modulation of miR-378a-3p and TLR8 expression to elucidate their roles.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The downregulation of miR-378a-3p in stroke (n = 82) compared to controls (n = 50) was observed, as well as in PSCI patients (n = 40) compared with that in post-stroke cognitively normal (n = 42). ROC curve analysis identified the potential of miR-378a-3p as a diagnostic biomarker for stroke and PSCI, yielding an area under the ROC curve (AUC) of 0.9039 (sensitivity: 89.02%; specificity: 72.00%). It was correlated positively with MoCA scores. ROC curve analysis resulted in an AUC of 0.8092 (sensitivity: 52.50%; specificity: 95.24%) for miR-378a-3p in predicting PSCI. miR-378a-3p had potential to predict PSCI occurrence. Luciferase assays confirmed direct binding between miR-378a-3p and TLR8. Overexpressing miR-378a-3p enhanced cell survival under OGD conditions, which was reversed by overexpressing TLR8.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Reduced circulating miR-378a-3p associated with cognitive decline post-stroke, by negatively regulating TLR8.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/08919887261487671","type":"journal-article","created":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T13:51:41Z","timestamp":1789048301000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Predictive Value of Abnormal Expression of miR-378a-3p in Elderly Stroke Patients for Cognitive Impairment After Stroke"],"prefix":"10.1177","author":[{"given":"Yan","family":"Xiao","sequence":"first","affiliation":[{"name":"Affiliated Hospital of Inner Mongolia Medical University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Yang","sequence":"additional","affiliation":[{"name":"Taicang Hospital Affiliated to Nanjing University of Chinese Medicine\/Taicang Traditional Chinese Medicine Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiuyuan","family":"Zhao","sequence":"additional","affiliation":[{"name":"The Second Hospital of Tianjin Medical University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-3770-6632","authenticated-orcid":false,"given":"Haining","family":"Zhen","sequence":"additional","affiliation":[{"name":"Xijing Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoquan","family":"Li","sequence":"additional","affiliation":[{"name":"People\u2019s Hospital of Longhua"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,9,10]]},"reference":[{"key":"e_1_3_3_2_2","first-page":"393","volume-title":"Handbook of Clinical Neurology","author":"Sharrief A","year":"2019","unstructured":"Sharrief A, Grotta JC. 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