{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T03:56:38Z","timestamp":1782878198730,"version":"3.54.5"},"reference-count":17,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2020,3,12]],"date-time":"2020-03-12T00:00:00Z","timestamp":1583971200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100001343","name":"University of Pretoria","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001343","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Perit Dial Int"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>Peritoneal dialysis (PD) peritonitis is a feared complication of PD, with significant sequelae for the patient. The cause of PD peritonitis is largely due to a single organism (\u226575% of cases) and rarely due to multiple organisms.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>In this pilot study, we investigated 25 cases of PD peritonitis with 16S ribosomal RNA (rRNA) next-generation sequencing (NGS) techniques.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>Total concordance between culture and NGS was noted. In addition, the NGS technique was highly sensitive, identifying 33 different bacteria (including a nonculturable bacterium), compared to 13 bacterial species using culture-based techniques. This was counterbalanced by a lack of specificity with NGS, largely due to the small size of the 16S rRNA gene segment sequenced.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>For the clinician, our results suggest that PD peritonitis may often be a polymicrobial disease and that treating a dominant organism may not totally eradicate all bacterial contamination within the peritoneum. For the clinical scientist, additional use of a larger 16S rRNA segment (V5 or V6) is likely to outperform the use of the V4 segment only.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/0896860820908473","type":"journal-article","created":{"date-parts":[[2020,3,12]],"date-time":"2020-03-12T06:19:06Z","timestamp":1583993946000},"page":"581-586","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":14,"title":["Bacterial pathogens in peritoneal dialysis peritonitis: Insights from next-generation sequencing"],"prefix":"10.1177","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4625-0139","authenticated-orcid":false,"given":"Wesley G","family":"van Hougenhouck-Tulleken","sequence":"first","affiliation":[{"name":"Department of Nephrology, Steve Biko Academic Hospital, Pretoria, South Africa"},{"name":"Department of Internal Medicine, University of Pretoria, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro H","family":"Lebre","sequence":"additional","affiliation":[{"name":"Centre for Microbial Ecology and Genomics, University of Pretoria, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed","family":"Said","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, University of Pretoria, South Africa"},{"name":"National Health Laboratory Services, Tshwane Academic Division, University of Pretoria, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Don A","family":"Cowan","sequence":"additional","affiliation":[{"name":"Centre for Microbial Ecology and Genomics, University of Pretoria, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2020,3,12]]},"reference":[{"key":"bibr1-0896860820908473","doi-asserted-by":"publisher","DOI":"10.1159\/000437286"},{"key":"bibr2-0896860820908473","first-page":"51","volume":"20","author":"Wagner G","year":"1877","journal-title":"Arch Klin Chir"},{"key":"bibr3-0896860820908473","doi-asserted-by":"publisher","DOI":"10.1097\/00005792-196109000-00001"},{"key":"bibr4-0896860820908473","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1992.375"},{"key":"bibr5-0896860820908473","doi-asserted-by":"publisher","DOI":"10.3747\/pdi.2016.00078"},{"key":"bibr6-0896860820908473","doi-asserted-by":"publisher","DOI":"10.2215\/CJN.00630117"},{"key":"bibr7-0896860820908473","doi-asserted-by":"publisher","DOI":"10.3747\/pdi.2010.00131"},{"key":"bibr8-0896860820908473","doi-asserted-by":"publisher","DOI":"10.1128\/JB.180.18.4765-4774.1998"},{"key":"bibr9-0896860820908473","doi-asserted-by":"publisher","DOI":"10.1038\/nature11553"},{"key":"bibr10-0896860820908473","first-page":"16","volume":"243","author":"Raangs EC","year":"2016","journal-title":"J Biotechnol"},{"key":"bibr11-0896860820908473","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.f.303"},{"key":"bibr12-0896860820908473","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"bibr13-0896860820908473","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.03006-05"},{"key":"bibr14-0896860820908473","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gki025"},{"key":"bibr15-0896860820908473","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.12.5940-5943.2004"},{"key":"bibr16-0896860820908473","doi-asserted-by":"publisher","DOI":"10.1186\/s12859-016-0992-y"},{"key":"bibr17-0896860820908473","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2018.04.3634"}],"container-title":["Peritoneal Dialysis International: Journal of the International Society for Peritoneal Dialysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0896860820908473","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/0896860820908473","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0896860820908473","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T22:32:57Z","timestamp":1777674777000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/0896860820908473"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,3,12]]},"references-count":17,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2020,11]]}},"alternative-id":["10.1177\/0896860820908473"],"URL":"https:\/\/doi.org\/10.1177\/0896860820908473","relation":{},"ISSN":["0896-8608","1718-4304"],"issn-type":[{"value":"0896-8608","type":"print"},{"value":"1718-4304","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,3,12]]}}}