{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T06:01:16Z","timestamp":1787983276552,"version":"build-2784847793"},"reference-count":22,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2020,4,10]],"date-time":"2020-04-10T00:00:00Z","timestamp":1586476800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100004853","name":"Chinese University of Hong Kong","doi-asserted-by":"publisher","award":["research accounts 6901031 and 7101215"],"award-info":[{"award-number":["research accounts 6901031 and 7101215"]}],"id":[{"id":"10.13039\/501100004853","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Perit Dial Int"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>Peritoneal dialysis (PD)-related peritonitis is a serious complication of PD, but routine microbiological culture is slow and could not identify the organism in 15% cases. We examine the accuracy of polymerase chain reaction\/electrospray ionization\u2013mass spectrometry (PCR\/ESI-MS), a PCR-based method developed for the direct detection of bacteria in blood, for rapid identification of microorganisms from PD effluent.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>We recruited 73 consecutive patients with PD-related peritonitis. Dialysis effluent was collected for routine bacterial culture, PCR\/ESI-MS, and bacterial DNA quantification before initiation of antibiotic therapy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>By digital PCR with universal bacterial primers, bacterial DNA was detectable in all PD effluent specimens. For the entire cohort, taking standard bacterial culture as the gold standard, the PCR\/ESI-MS assay correctly identified 34.3% of the causative organisms, failed to identify any organism in 52.1% cases, and identified a different organism in 8.2% cases. For the 14 episodes of peritonitis that were culture negative by conventional bacterial culture, the PCR\/ESI-MS assay identified an organism in only four cases. The detection rate of the IRIDICA BAC BSI assay was not affected by the use of biocompatible PD solution or concomitant exit-site infection.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>The PCR\/ESI-MS assay could not identify the causative organism in over 50% of the PD effluent samples in patients with PD-related peritonitis and should be not used for such purpose. The reason for the poor performance needs further investigation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/0896860820917845","type":"journal-article","created":{"date-parts":[[2020,4,10]],"date-time":"2020-04-10T07:38:30Z","timestamp":1586504310000},"page":"96-100","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":6,"title":["Polymerase chain reaction\/electrospray ionization\u2013mass spectrometry (PCR\/ESI-MS) is not suitable for rapid bacterial identification in peritoneal dialysis effluent"],"prefix":"10.1177","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8898-8505","authenticated-orcid":false,"given":"Cheuk Chun","family":"Szeto","sequence":"first","affiliation":[{"name":"Department of Medicine and Therapeutics, Carol and Richard Yu Peritoneal Dialysis Research Centre, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China"},{"name":"Li Ka Shing Institute of Health Sciences (LiHS), Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jack Kit-Chung","family":"Ng","sequence":"additional","affiliation":[{"name":"Department of Medicine and Therapeutics, Carol and Richard Yu Peritoneal Dialysis Research Centre, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Winston Wing-Shing","family":"Fung","sequence":"additional","affiliation":[{"name":"Department of Medicine and Therapeutics, Carol and Richard Yu Peritoneal Dialysis Research Centre, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ka-Bik","family":"Lai","sequence":"additional","affiliation":[{"name":"Department of Medicine and Therapeutics, Carol and Richard Yu Peritoneal Dialysis Research Centre, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China"},{"name":"Li Ka Shing Institute of Health Sciences (LiHS), Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai-Ming","family":"Chow","sequence":"additional","affiliation":[{"name":"Department of Medicine and Therapeutics, Carol and Richard Yu Peritoneal Dialysis Research Centre, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip Kam-Tao","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Medicine and Therapeutics, Carol and Richard Yu Peritoneal Dialysis Research Centre, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arikana","family":"Massiah","sequence":"additional","affiliation":[{"name":"Microbiology, Barts Health NHS Trust, London, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adela","family":"Alcolea-Medina","sequence":"additional","affiliation":[{"name":"Microbiology, Barts Health NHS Trust, London, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Wilks","sequence":"additional","affiliation":[{"name":"Microbiology, Barts Health NHS Trust, London, UK"},{"name":"Blizard Institute, Queen Mary University of London, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stanley L","family":"Fan","sequence":"additional","affiliation":[{"name":"Renal Medicine and Transplantation, Barts Health NHS Trust, London, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2020,4,10]]},"reference":[{"key":"bibr1-0896860820917845","doi-asserted-by":"publisher","DOI":"10.3747\/pdi.2011.00233"},{"key":"bibr2-0896860820917845","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-22335-4"},{"key":"bibr3-0896860820917845","doi-asserted-by":"publisher","DOI":"10.3747\/pdi.2016.00078"},{"key":"bibr4-0896860820917845","doi-asserted-by":"publisher","DOI":"10.1007\/s10156-010-0069-1"},{"key":"bibr5-0896860820917845","doi-asserted-by":"publisher","DOI":"10.1016\/S0272-6386(03)00790-X"},{"key":"bibr6-0896860820917845","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0158186"},{"key":"bibr7-0896860820917845","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00801-14"},{"key":"bibr8-0896860820917845","doi-asserted-by":"publisher","DOI":"10.1586\/erm.10.24"},{"key":"bibr9-0896860820917845","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00966-13"},{"key":"bibr10-0896860820917845","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0036528"},{"key":"bibr11-0896860820917845","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-60327-999-4_7"},{"key":"bibr12-0896860820917845","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1918"},{"key":"bibr13-0896860820917845","doi-asserted-by":"publisher","DOI":"10.2215\/CJN.02360213"},{"key":"bibr14-0896860820917845","first-page":"69","volume":"21","author":"Park 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