{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T03:03:29Z","timestamp":1788404609502,"version":"build-2803163510"},"reference-count":32,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T00:00:00Z","timestamp":1776729600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100004281","name":"Narodowe Centrum Nauki","doi-asserted-by":"publisher","award":["OPUS UMO-2017\/27\/B\/NZ4\/01675"],"award-info":[{"award-number":["OPUS UMO-2017\/27\/B\/NZ4\/01675"]}],"id":[{"id":"10.13039\/501100004281","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Perit Dial Int"],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Peritoneal dialysis induces an intraperitoneal inflammatory reaction, which causes damage to the peritoneum. Inflammation also accelerates cellular senescence. We studied in vitro effect of the dialysates from peritoneal dialysis patients on the senescence of the peritoneal mesothelial cells (MCs). The effect of N-acetylcysteine (NAC) on that process was studied.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Replicative senescence was induced in MC cells exposed to culture medium, medium mixed with the dialysate \u00b1 NAC 0.025\u2005mmol\/L. After 10 passages, markers of the cellular senescence and secretory activity of the cells were measured. Additionally, the effect of NAC on the senescent cells was studied.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Exposure of MC to the dialysate accelerated, more than in medium alone, their senescence as reflected by elongation of the population doubling time, increased expression of p21, p53 genes and \u03b2-galactosidase activity. Secretion of IL6 and transforming growth factor \u03b2 (TGF\u03b2) was increased, and fibrinolytic activity, as reflected by the tissue plasminogen activator\/plasminogen activator inhibitor-1 ratio, was reduced. NAC slowed down the process of senescence in MC treated with the dialysate. NAC suppressed the proinflammatory properties of the senescent MC.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The proinflammatory properties of the peritoneal dialysate accelerate the senescence of MC. Decreased fibrinolytic activity of MC, increased secretion of IL6 and TGF\u03b2 may accelerate fibrosis of the peritoneum. Supplementation of NAC in patients treated with peritoneal dialysis may help preserve the peritoneum as the dialysis membrane.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/08968608261443578","type":"journal-article","created":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T13:29:34Z","timestamp":1776778174000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Antisenescent and antiinflammatory effect of N-acetylcysteine in peritoneal mesothelial cells"],"prefix":"10.1177","author":[{"given":"Zofia","family":"Strojny","sequence":"first","affiliation":[{"name":"Poznan University of Medical Sciences"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wies\u0142aw","family":"Sikora","sequence":"additional","affiliation":[{"name":"Poznan University of Medical Sciences"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna","family":"Hoffmann-Aulich","sequence":"additional","affiliation":[{"name":"University of Zielona G\u00f3ra"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominika","family":"Kanikowska","sequence":"additional","affiliation":[{"name":"Poznan University of Medical Sciences"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0916-623X","authenticated-orcid":false,"given":"Andrzej","family":"Br\u0119borowicz","sequence":"additional","affiliation":[{"name":"Poznan University of Medical Sciences"},{"name":"University of Zielona G\u00f3ra"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,4,21]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25168607"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/sdi.12579"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.3747\/pdi.2017.00244"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/aor.14001"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/sdi.12818"},{"key":"e_1_3_3_7_2","first-page":"943","article-title":"L-2-oxothiazolidine-4-carboxylic acids slow down dialysate-induced senescence in human peritoneal mesothelial cells","volume":"69","author":"Sosinska-Zawierucha P","year":"2018","unstructured":"Sosinska-Zawierucha P, Mackowiak B, Begier-Krasinska B, et al. L-2-oxothiazolidine-4-carboxylic acids slow down dialysate-induced senescence in human peritoneal mesothelial cells. J Physiol Pharmacol 2018; 69: 943\u2013950.","journal-title":"J Physiol Pharmacol"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00009.2009"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.5301\/JN.2011.8404"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/642793"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.surg.2011.02.015"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.3747\/pdi.2009.00073"},{"key":"e_1_3_3_13_2","first-page":"140","article-title":"Effect of oral N-acetylcysteine treatment on immune system in continuous ambulatory peritoneal dialysis patients","volume":"44","author":"Purwanto B","year":"2012","unstructured":"Purwanto B, Prasetyo DH. Effect of oral N-acetylcysteine treatment on immune system in continuous ambulatory peritoneal dialysis patients. Acta Med Indones 2012; 44: 140\u2013144.","journal-title":"Acta Med Indones"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.3747\/pdi.2009.00263"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1159\/000170234"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.20.9363"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.3390\/antiox14080987"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/3494867"},{"key":"e_1_3_3_20_2","first-page":"138","article-title":"A single administration of peritoneal dialysis fluid in the rat induces an acute inflammatory exudate","volume":"7","author":"Beelen RH","year":"1991","unstructured":"Beelen RH, Bos HJ, Meyer F, et al. 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