{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T08:12:18Z","timestamp":1778227938384,"version":"3.51.4"},"reference-count":19,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T00:00:00Z","timestamp":1777939200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"Yiming Zhang","award":["2024-RC(BS)-005"],"award-info":[{"award-number":["2024-RC(BS)-005"]}]},{"name":"Xiaofen Ma","award":["RCTAIIRIGAN039"],"award-info":[{"award-number":["RCTAIIRIGAN039"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Perit Dial Int"],"abstract":"<jats:p>\n                    Brucellosis is an infectious disease characterized by a natural foci, caused by\n                    <jats:italic toggle=\"yes\">Brucella<\/jats:italic>\n                    , a genus of Gram-negative bacilli known for its obligate aerobic growth and capability for intracellular parasitism. This pathogen can be transmitted zoonotically between animals and humans. Due to its mechanism of intracellular survival, monotherapy with a single antibiotic often proves ineffective in eradicating\n                    <jats:italic toggle=\"yes\">Brucella<\/jats:italic>\n                    . Therefore, clinical management necessitates the implementation of multidrug combination therapy alongside extended therapeutic regimens to achieve optimal bacteriological clearance. Conventional diagnostic methods, such as bacterial culture and serological testing, are frequently hindered by the fastidious growth requirements of the pathogen and the risk of false-positive serological cross-reactivity. These limitations may result in delayed diagnosis or unnecessary interventions. Recent advancements in molecular biology\u2014particularly genomic analysis technologies\u2014have revolutionized pathogen detection by facilitating rapid and precise identification of elusive pathogens. This study reports the first documented case of refractory peritoneal dialysis-associated peritonitis caused by\n                    <jats:italic toggle=\"yes\">Brucella<\/jats:italic>\n                    diagnosed via metagenomic next-generation sequencing (mNGS). By employing mNGS to identify the pathogen, we summarize the clinical characteristics of this case and highlight the diagnostic advantages of this technology. Our findings aim to guide clinicians in selecting appropriate diagnostic approaches for similar cases, avoiding unnecessary delays and optimizing resource utilization in clinical practice.\n                  <\/jats:p>","DOI":"10.1177\/08968608261448060","type":"journal-article","created":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T13:35:34Z","timestamp":1777988134000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["A wolf in sheep's clothing: An unusual zoonotic pathogen in peritoneal dialysis effluent and an important diagnostic strategy"],"prefix":"10.1177","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-6567-5291","authenticated-orcid":false,"given":"Chen","family":"Wang","sequence":"first","affiliation":[{"name":"Affiliated Hospital of Jining Medical University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-2016-055X","authenticated-orcid":false,"given":"Pan","family":"Dou","sequence":"additional","affiliation":[{"name":"Jining Medical University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-6461-7303","authenticated-orcid":false,"given":"Yanyan","family":"Wang","sequence":"additional","affiliation":[{"name":"Affiliated Hospital of Jining Medical University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4826-6070","authenticated-orcid":false,"given":"Xiang","family":"Li","sequence":"additional","affiliation":[{"name":"Affiliated Hospital of Jining Medical University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-9345-6125","authenticated-orcid":false,"given":"Yiming","family":"Zhang","sequence":"additional","affiliation":[{"name":"Affiliated Hospital of Jining Medical University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-1775-2083","authenticated-orcid":false,"given":"Xiaofen","family":"Ma","sequence":"additional","affiliation":[{"name":"Affiliated Hospital of Jining Medical University"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2026,5,5]]},"reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2024.1393548"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.3747\/pdi.2018.00115"},{"key":"e_1_3_2_4_2","first-page":"415","article-title":"Current therapeutic strategy in osteoarticular brucellosis","volume":"6","author":"Unuvar GK","year":"2019","unstructured":"Unuvar GK, Kilic AU, Doganay M. Current therapeutic strategy in osteoarticular brucellosis. North Clin Istanb 2019; 6: 415\u2013420.","journal-title":"North Clin Istanb"},{"key":"e_1_3_2_5_2","article-title":"Brucella peritonitis in a patient on peritoneal dialysis: a case report and review of literature","volume":"13","author":"Al ZS","year":"2021","unstructured":"Al ZS, Rubaihan AK, Alnetaifat MF, et al. Brucella peritonitis in a patient on peritoneal dialysis: a case report and review of literature. 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Medicine (Baltimore) 2025; 104: e41481.","journal-title":"Medicine (Baltimore)"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S436278"},{"key":"e_1_3_2_13_2","first-page":"11","article-title":"The development of diagnostic and vaccine strategies for early detection and control of human brucellosis, particularly in endemic areas","author":"Elbehiry A","year":"2023","unstructured":"Elbehiry A, Aldubaib M, Marzouk E, et al. The development of diagnostic and vaccine strategies for early detection and control of human brucellosis, particularly in endemic areas. Vaccines (Basel) 2023: 11(3): 654.","journal-title":"Vaccines (Basel)"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-024-69669-w"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0011590"},{"key":"e_1_3_2_16_2","first-page":"13","article-title":"Bacterial ghosts-based vaccine and drug delivery systems","author":"Chen H","year":"2021","unstructured":"Chen H, Ji H, Kong X, et al. Bacterial ghosts-based vaccine and drug delivery systems. Pharmaceutics 2021: 13(11): 1892.","journal-title":"Pharmaceutics"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.2147\/DDDT.S341242"},{"key":"e_1_3_2_18_2","first-page":"661","article-title":"Diagnosis and treatment scheme for brucellosis (2023 edition)","volume":"23","year":"2024","unstructured":"Diagnosis and treatment scheme for brucellosis (2023 edition). 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