{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T01:38:59Z","timestamp":1777858739412,"version":"3.51.4"},"reference-count":45,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2019,12,1]],"date-time":"2019-12-01T00:00:00Z","timestamp":1575158400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"Scientific Research Project Fund of Cumhuriyet University","award":["Project number Dis 102"],"award-info":[{"award-number":["Project number Dis 102"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Vet Dent"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:p>The purpose of this study was to evaluate the biochemical, morphometric, and histopathological changes associated with experimental periodontitis in rats in response to local administration of humic acid. Thirty-eight Wistar rats were divided into 5 experimental groups: nonligated (NL) group, ligature-only (LO) group, and ligature + local administration of humic acid (20, 80, and 150 mg\/kg body weight per day for 15 days, respectively; L-20, L-80, and L-150 groups). Changes in alveolar bone levels were clinically measured as the distance from the cementoenamel junction to the alveolar bone crest with a stereomicroscope. Tissues were histopathologically examined to assess the osteoclast numbers, osteoblastic activity, and inflammatory cell infiltration among the study groups. Enzyme-linked immunosorbent assay interleukin1\u03b2 (IL-1\u03b2) and IL-10 levels in serum and gingival homogenates were evaluated. At the end of 15 days, the alveolar bone loss was significantly higher in the LO group compared to the NL, L-20, and L-150 groups ( P &lt; .05). The osteoclast number in the LO group was significantly higher than the NL, L-20, and L-150 groups ( P &lt; .05). Inflammatory cell infiltration was significantly higher in the LO and L-80 groups than the other groups ( P &lt; .05). The highest serum and gingival homogenate IL-10 levels were determined in the NL group ( P &lt; .05). The serum and gingival homogenate IL-1\u03b2 levels in LO group were significantly higher than the NL, L-20, and L-150 groups ( P &lt; .05). Within the limits of this study, it can be suggested that humic acid, when administered locally at 20 and 80 mg\/kg doses, may prevent alveolar bone loss in the rat model.<\/jats:p>","DOI":"10.1177\/0898756420910531","type":"journal-article","created":{"date-parts":[[2020,3,24]],"date-time":"2020-03-24T06:41:45Z","timestamp":1585032105000},"page":"257-265","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":7,"title":["Humic Acid, a Polyphenolic Substance, Decreases Alveolar Bone Loss in Experimental Periodontitis in Rats"],"prefix":"10.1177","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6607-6532","authenticated-orcid":false,"given":"Metin","family":"\u00c7al\u0131\u015f\u0131r","sequence":"first","affiliation":[{"name":"Department of Periodontology, Faculty of Dentistry, Ad\u0131yaman University, Ad\u0131yaman, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aysun","family":"Akp\u0131nar","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Faculty of Dentistry, Cumhuriyet University, Sivas, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00d6mer","family":"Poyraz","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine, Cumhuriyet University, Sivas, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fahrettin","family":"G\u00f6ze","sequence":"additional","affiliation":[{"name":"Department of Pathology, Faculty of Medicine, Cumhuriyet University, Sivas, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ziynet","family":"\u00c7\u0131nar","sequence":"additional","affiliation":[{"name":"Department of Biostatistics, Faculty of Medicine, Cumhuriyet University, Sivas, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2020,3,24]]},"reference":[{"issue":"2","key":"bibr1-0898756420910531","first-page":"169","volume":"36","author":"K\u00fchnert M","year":"1982","journal-title":"Arch Exp Veterinarmed"},{"key":"bibr2-0898756420910531","unstructured":"Kl\u00f6cking R, Helbig B. 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