{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:55:23Z","timestamp":1777924523337,"version":"3.51.4"},"reference-count":55,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Technology and Health Care"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Bortezomib is the first-line drug in the treatment of multiple myeloma (MM) and its resistance is the main obstacle to cure MM. MicroRNA-361-5p (MiRNA-361-5p) in bone marrow mesenchymal stem cells (BMSCs) might participate in the bortezomib resistance via paracrine pathway. The study was to characterize the role and molecular mechanism of miR-361-5p in bortezomib resistance in MM.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The exosomes of BMSCs were obtained and characterized by transmission electron microscopy and nanoparticle tracking analysis. The MM cell U266 was treated with bortezomib, bortezomib and BMSC exosomes, bortezomib and BMSC exosomes transfected with miR-361-5p inhibitor. The cell viability was measured by cell counting kit. Protein expression of PDPK1, PI3K, p-PI3K, mTOR, p-mTOR, AKT, and Pan-AKT was detected by western blot. The apoptosis level of bortezomib resistant cell lines was detected by flow cytometry.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Low expression of miR-361-5p promoted the survival of U266 cells and inhibited cell apoptosis, reversing the inhibitory effect of bortezomib on U266 cells. PDPK1 may be a downstream target of miR-361-5p. Low expression of BMSCs-derived exosomal miR-361-5p may reverse the effect of bortezomib on U266 cells by regulating the PDPK1\/PI3K\/AKT\/mTOR axis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Low expression of BMSCs-derived exosomal miR-361-5p may overcome bortezomib resistance in MM by regulating PDPK1\/PI3K\/AKT\/mTOR axis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/09287329251350918","type":"journal-article","created":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T08:11:46Z","timestamp":1754035906000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Bone marrow mesenchymal stem cell derived exosomal miR-361-5p reversed the effect of bortezomib on multiple myeloma"],"prefix":"10.1177","author":[{"given":"Tianbao","family":"Lin","sequence":"first","affiliation":[{"name":"Department of Medical Ultrasonics, The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine), Hangzhou City, Zhejiang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Hematology, The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine), Hangzhou City, Zhejiang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhiping","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Hematology, The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine), Hangzhou City, Zhejiang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuyan","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Hematology, The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine), Hangzhou City, Zhejiang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2025,8]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.1497"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12094-020-02541-1"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2020.561601"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.3390\/ph16010111"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22031341"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.cco.0000245320.34658.bd"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s40262-018-0679-9"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-06752-0"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-14-3400"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-386469-7.00002-5"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0268-960X(10)70003-5"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2020.608815"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2014-03-562439"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21207539"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-023-04874-1"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.molpharmaceut.1c00669"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2020.118821"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.202101868RRR"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2023.1149168"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/1575468"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2021.9632"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00381-019-04199-9"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13046-017-0630-1"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1097\/CAD.0000000000000846"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2021.10006"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.4014\/jmb.2010.10059"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.3802"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.29383"},{"key":"e_1_3_3_30_2","first-page":"365","article-title":"Exploration of mechanism of bortezomib on side population cell from multiple myeloma based on PI3K\/AKT\/mTOR","volume":"18","author":"Shuyan L","year":"2020","unstructured":"Shuyan L, Yun Z, Shengyun L. Exploration of mechanism of bortezomib on side population cell from multiple myeloma based on PI3K\/AKT\/mTOR. Chin J Gen Pract 2020; 18: 365.","journal-title":"Chin J Gen Pract"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41375-019-0493-x"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1209456"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.3233\/THC-230917"},{"key":"e_1_3_3_34_2","first-page":"1","volume-title":"The use of artificial intelligence in diagnostic medical imaging: systematic literature review2021 IEEE 21st International Conference on Bioinformatics and Bioengineering (BIBE)","author":"Hafizovi\u0107 L","year":"2021","unstructured":"Hafizovi\u0107 L, \u010cau\u0161evi\u0107 A, Deumi\u0107 A, et\u00a0al. The use of artificial intelligence in diagnostic medical imaging: systematic literature review. In: 2021 IEEE 21st International Conference on Bioinformatics and Bioengineering (BIBE), Kragujevac, Serbia, 2021, pp. 1\u20136."},{"key":"e_1_3_3_35_2","volume-title":"CMBEBIH 2017. IFMBE Proceedings","author":"Veljovi\u0107 E","unstructured":"Veljovi\u0107 E, \u0160pirtovi\u0107-Halilovi\u0107 S, Muratovi\u0107 S, et\u00a0al. Artificial neural network and docking study in design and synthesis of xanthenes as antimicrobial agents. In: Badnjevic A (ed.) CMBEBIH 2017. IFMBE Proceedings, vol. 62. Singapore: Springer, 2017, pp. 617\u2013626."},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1177\/09287329241292168"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.3233\/THC-231482"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.3390\/cells10112865"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2013.09.007"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.4161\/rna.26541"},{"key":"e_1_3_3_41_2","first-page":"240","article-title":"Differentially expressed microRNAs in exosomes of patients with breast cancer revealed by next\u2013generation sequencing","volume":"43","author":"Wu H","year":"2020","unstructured":"Wu H, Wang Q, Zhong H, et\u00a0al. Differentially expressed microRNAs in exosomes of patients with breast cancer revealed by next\u2013generation sequencing. Oncol Rep 2020; 43: 240\u2013250.","journal-title":"Oncol Rep"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2015.4411"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep23531"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.3390\/biom10091281"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1111\/cas.13711"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-57698-0"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA117.000667"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.11988"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2012-02-407999"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2017.121"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-021-03719-3"},{"key":"e_1_3_3_52_2","first-page":"2584","article-title":"Analyzing the disease module associated with osteosarcoma via a network- and pathway-based approach","volume":"16","author":"Zhang Y","year":"2018","unstructured":"Zhang Y, Yang F. Analyzing the disease module associated with osteosarcoma via a network- and pathway-based approach. Exp Ther Med 2018; 16: 2584\u20132592.","journal-title":"Exp Ther Med"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2015.03.014"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-06-1984"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.23513"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-14-1420"}],"updated-by":[{"DOI":"10.1177\/09287329251392360","type":"expression_of_concern","label":"Expression of concern","source":"publisher","updated":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T00:00:00Z","timestamp":1762905600000}}],"container-title":["Technology and Health Care"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/09287329251350918","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/09287329251350918","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/09287329251350918","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T21:26:05Z","timestamp":1777670765000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/09287329251350918"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8]]},"references-count":55,"alternative-id":["10.1177\/09287329251350918"],"URL":"https:\/\/doi.org\/10.1177\/09287329251350918","relation":{},"ISSN":["0928-7329","1878-7401"],"issn-type":[{"value":"0928-7329","type":"print"},{"value":"1878-7401","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8]]},"article-number":"09287329251350918"}}