{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T15:25:19Z","timestamp":1786721119519,"version":"build-2736575974"},"reference-count":42,"publisher":"SAGE Publications","issue":"8","license":[{"start":{"date-parts":[[2014,8,1]],"date-time":"2014-08-01T00:00:00Z","timestamp":1406851200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Proc Inst Mech Eng H"],"published-print":{"date-parts":[[2014,8]]},"abstract":"<jats:p>Bone loss associated with skeletal trauma or metabolic diseases often requires bone grafting. In such situations, a biomaterial is necessary for migrated cells to produce new tissue. In this study, agarose\u2013chitosan was implanted in the femoral condyle of New Zealand White rabbits that were divided into three groups: Group I was used as control; Groups II and III were used as implanted tissue with agarose\u2013chitosan and presenting empty defects, respectively. This study evaluated the agarose\u2013chitosan biocompatibility by determining the in vivo genotoxicity, oxidative stress balance that correlated with the hardness mechanical property. Moreover, the histopathological and quantitative elements analyzed by using inductively coupled plasma optical emission spectrometry were determined. After 30 days of implantation, the in vivo analysis of genotoxicity showed that agarose\u2013chitosan did not induce chromosome aberration or micronucleus damage. A significant decrease in thiobarbituric and acid-reactive substance was observed after agarose\u2013chitosan implantation in the bone tissue. Superoxide dismutase, catalase and glutathione peroxidase were significantly enhanced in agarose\u2013chitosan\u2013treated group compared with that of control group. A negative correlation coefficient of the mechanical property with malonyldialdehyde level was detected (R\u2009=\u2009\u22120.998). The histological study exhibited a significantly increased angiogenesis and newly formed tissue. No presence of inflammatory process, necrotic or fibrous tissue was detected. Major and trace elements such as Ca, P, Zn, Mg and Fe were increased significantly in the newly formed bone. These findings show that agarose\u2013chitosan biomaterial implantation might be effective for treating trauma and bone regeneration.<\/jats:p>","DOI":"10.1177\/0954411914547247","type":"journal-article","created":{"date-parts":[[2014,9,9]],"date-time":"2014-09-09T04:15:48Z","timestamp":1410236148000},"page":"800-809","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":7,"title":["Genotoxicity effect, antioxidant and biomechanical correlation: Experimental study of agarose\u2013chitosan bone graft substitute in New Zealand white rabbit model"],"prefix":"10.1177","volume":"228","author":[{"given":"Samira","family":"Jebahi","sequence":"first","affiliation":[{"name":"UMR CNRS 6226, University of Rennes 1, Rennes, France"},{"name":"Histology, Orthopaedic and Traumatology Laboratory, Faculty of Medicine of Sfax, University of Sfax, Sfax, Tunisia"},{"name":"Animal Ecophysiology Laboratory, Department of Life Sciences, Faculty 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