{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T17:39:20Z","timestamp":1770485960106,"version":"3.49.0"},"reference-count":29,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2002,6,1]],"date-time":"2002-06-01T00:00:00Z","timestamp":1022889600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"},{"start":{"date-parts":[[2002,6,1]],"date-time":"2002-06-01T00:00:00Z","timestamp":1022889600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Antivir Chem Chemother"],"published-print":{"date-parts":[[2002,6]]},"abstract":"<jats:p>EM2487, a Streptomyces-derived substance, has previously been shown to inhibit HIV-1 replication in both acutely and chronically infected cells. In this study, we found that EM2487 was also a selective inhibitor of human T-lymphotropic virus type I (HTLV-I) replication in persistently infected cells. Its 50% effective concentrations for HTLV-I p19 antigen production were 3.6 and 1.2 \u03bcM in MT-2 and MT-4 cells, respectively. However, the compound did not reduce cell proliferation and viability at these concentrations. The 50% cytotoxic concentrations of EM2487 were 30.6 and 5.7 \u03bcM in MT-2 and MT-4 cells, respectively. The compound also displayed selective inhibition of HTLV-I production in peripheral blood mononuclear cells obtained from patients with HTLV-I-associated myelopathy\/tropical spastic paraparesis. Quantitative reverse transcription PCR analysis revealed that EM2487 selectively suppressed HTLV-I mRNA synthesis in MT-2 cells in a dose-dependent fashion. However, the compound did not inhibit endogenous Tax-induced HTLV-I long terminal repeat-driven reporter gene expression. Furthermore, intracellular Tax accumulation was not suppressed in MT-2 cells exposed to EM2487. These results suggest that the inhibition occurred at the viral transcription level, but it cannot be attributed to the inhibition of the Tax function.<\/jats:p>","DOI":"10.1177\/095632020201300304","type":"journal-article","created":{"date-parts":[[2015,3,16]],"date-time":"2015-03-16T17:01:37Z","timestamp":1426525297000},"page":"177-183","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":5,"title":["Inhibition of Human T-Lymphotropic Virus Type I Gene Expression by the Streptomyces-Derived Substance EM2487"],"prefix":"10.1177","volume":"13","author":[{"given":"X","family":"Wang","sequence":"first","affiliation":[{"name":"Division of Human Retroviruses Center for Chronic Viral Diseases, Faculty of Medicine, Kagoshima University, Kagoshima, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Okamoto","sequence":"additional","affiliation":[{"name":"Division of Human Retroviruses Center for Chronic Viral Diseases, Faculty of Medicine, Kagoshima University, Kagoshima, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Kawamura","sequence":"additional","affiliation":[{"name":"Division of Human Retroviruses Center for Chronic Viral Diseases, Faculty of Medicine, Kagoshima University, Kagoshima, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Izumo","sequence":"additional","affiliation":[{"name":"Division of Molecular Pathology and Genetic Epidemiology, Center for Chronic Viral Diseases, Faculty of Medicine, Kagoshima University, Kagoshima, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Baba","sequence":"additional","affiliation":[{"name":"Division of Human Retroviruses Center for Chronic Viral Diseases, Faculty of Medicine, Kagoshima University, Kagoshima, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2002,6,1]]},"reference":[{"key":"bibr1-095632020201300304","doi-asserted-by":"crossref","first-page":"1097","DOI":"10.1016\/S0026-895X(24)13282-8","volume":"53","author":"Baba M","year":"1998","journal-title":"Molecular Pharmacology"},{"key":"bibr2-095632020201300304","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.10.2350"},{"key":"bibr3-095632020201300304","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.162.3.1765"},{"key":"bibr4-095632020201300304","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(95)90437-9"},{"key":"bibr5-095632020201300304","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.72.11.8627-8635.1998"},{"key":"bibr6-095632020201300304","first-page":"1849","volume-title":"Fields Virology","author":"Cann AJ","year":"1996"},{"key":"bibr7-095632020201300304","doi-asserted-by":"publisher","DOI":"10.1126\/science.2990037"},{"key":"bibr8-095632020201300304","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(85)90405-2"},{"key":"bibr9-095632020201300304","doi-asserted-by":"publisher","DOI":"10.1126\/science.2992082"},{"key":"bibr10-095632020201300304","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V86.10.3619.bloodjournal86103619"},{"key":"bibr11-095632020201300304","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.63.8.3234-3239.1989"},{"key":"bibr12-095632020201300304","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(85)92734-5"},{"key":"bibr13-095632020201300304","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1988.tb02840.x"},{"key":"bibr14-095632020201300304","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.11.3653"},{"key":"bibr15-095632020201300304","doi-asserted-by":"publisher","DOI":"10.1016\/0022-510X(92)90215-7"},{"key":"bibr16-095632020201300304","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19871201)60:11<2605::AID-CNCR2820601104>3.0.CO;2-T"},{"key":"bibr17-095632020201300304","doi-asserted-by":"publisher","DOI":"10.1038\/294770a0"},{"key":"bibr18-095632020201300304","first-page":"219","volume":"28","author":"Miyoshi I","year":"1982","journal-title":"Japanese Journal of Cancer Research"},{"key":"bibr19-095632020201300304","doi-asserted-by":"publisher","DOI":"10.3109\/13550289809114225"},{"key":"bibr20-095632020201300304","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(86)91298-5"},{"key":"bibr21-095632020201300304","doi-asserted-by":"publisher","DOI":"10.1016\/0166-0934(88)90134-6"},{"key":"bibr22-095632020201300304","doi-asserted-by":"publisher","DOI":"10.1097\/00042560-199910010-00014"},{"key":"bibr23-095632020201300304","doi-asserted-by":"publisher","DOI":"10.7164\/antibiotics.52.971"},{"key":"bibr24-095632020201300304","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.1990.tb02554.x"},{"key":"bibr25-095632020201300304","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.73.12.10289-10295.1999"},{"key":"bibr26-095632020201300304","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V50.3.481.481"},{"key":"bibr27-095632020201300304","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410210204"},{"key":"bibr28-095632020201300304","doi-asserted-by":"publisher","DOI":"10.1124\/mol.61.6.1359"},{"key":"bibr29-095632020201300304","first-page":"145","volume":"907","author":"Yoshida M","year":"1987","journal-title":"Biochimica et Biophysica Acta"}],"container-title":["Antiviral Chemistry and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/095632020201300304","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/095632020201300304","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,21]],"date-time":"2025-05-21T00:45:31Z","timestamp":1747788331000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/095632020201300304"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,6]]},"references-count":29,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2002,6]]}},"alternative-id":["10.1177\/095632020201300304"],"URL":"https:\/\/doi.org\/10.1177\/095632020201300304","relation":{},"ISSN":["0956-3202","2040-2066"],"issn-type":[{"value":"0956-3202","type":"print"},{"value":"2040-2066","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,6]]}}}