{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T15:14:28Z","timestamp":1783610068144,"version":"3.55.0"},"reference-count":31,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2005,10,1]],"date-time":"2005-10-01T00:00:00Z","timestamp":1128124800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"},{"start":{"date-parts":[[2005,10,1]],"date-time":"2005-10-01T00:00:00Z","timestamp":1128124800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Antivir Chem Chemother"],"published-print":{"date-parts":[[2005,10]]},"abstract":"<jats:p> To improve anti-helical activity of analogues of 1 H-benzotriazole and 1 H-benzimidazole their N-alkyl derivatives were synthesized and tested for anti-helicase activity against enzymes of selected Flaviviridae including hepatitis C virus (HCV), West Nile virus (WNV), Dengue virus (DENV) and Japanese encephalitis virus (JEV). 1- and 2-alkyl derivatives of 4,5,6,7-tetrabromo-1 H-benzotriazole were obtained by direct alkylation of 4,5,6,7-tetrabromo-1 H-benzotriazole with the use of respective alkyl halides in the presence of KOH in methanol, to give a mixture of 1- and 2- isomers, which was separated by flash column chromatography in good yield. The proportion of isomers strongly depended on the reaction time and temperature. 1- and 2-hydroxyethyl and 1- and 2-chloroethyl derivatives of the tetrabromobenzo-triazole were synthesized with the use of 2-bromoethanol and 1-bromo-2-chloroethane respectively as alkylating agents. N-alkylation of this benzotriazole compound enhanced inhibitory activity and selectivity towards the helicase activity of HCV NTPase\/helicase. The most active were the 2-methyl, 2-ethyl and 2-propyl derivatives (IC<jats:sub>50<\/jats:sub>\u223c6.5 \u03bcM in the presence of DNA as a substrate). Derivatives of the benzotriazole in which hydroxyethyl or chloroethyl replaced the alkyl substituents lost their inhibitory activity. Brominated or methylated benzotriazole N(1) ribosides also did not exert helicase inhibitory activity. Although a number of N(1) and N(2) alkyl derivatives exerted good HCV and WNV helicase inhibitory activity when DNA was used as substrate, the activity was strongly decreased or even disappeared when RNA was used as substrate. The cytotoxicity tests in Vero and HeLa Tat cells showed a substantial decrease of cytotoxicity of N-alkyl derivatives as compared to the parent benzotriazole. <\/jats:p>","DOI":"10.1177\/095632020501600504","type":"journal-article","created":{"date-parts":[[2015,3,16]],"date-time":"2015-03-16T17:01:37Z","timestamp":1426525297000},"page":"315-326","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":44,"title":["Synthesis and Biological Activity of 1<i>H<\/i>-benzotriazole and 1<i>H<\/i>-benzimidazole Analogues \u2014 Inhibitors of the NTPase\/Helicase of HCV and of Some Related <i>Flaviviridae<\/i>"],"prefix":"10.1177","volume":"16","author":[{"given":"Maria","family":"Bretner","sequence":"first","affiliation":[{"name":"Instytut Biochemii i Biofizyki, Polska Akademia Nauk, Warszawa, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Baier","sequence":"additional","affiliation":[{"name":"Bernhard-Nocht-Institut f\u00fcr Tropenmedizin, Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katarzyna","family":"Kopa\u0144ska","sequence":"additional","affiliation":[{"name":"Instytut Biochemii i Biofizyki, Polska Akademia Nauk, Warszawa, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"And\u017celika","family":"Najda","sequence":"additional","affiliation":[{"name":"Instytut Biochemii i Biofizyki, Polska Akademia Nauk, Warszawa, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Schoof","sequence":"additional","affiliation":[{"name":"Bernhard-Nocht-Institut f\u00fcr Tropenmedizin, Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Reinholz","sequence":"additional","affiliation":[{"name":"Bernhard-Nocht-Institut f\u00fcr Tropenmedizin, Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrzej","family":"Lipniacki","sequence":"additional","affiliation":[{"name":"Instytut Medycyny Doswiadczalnej i Klinicznej, Polska Akademia Nauk, Warszawa, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrzej","family":"Piasek","sequence":"additional","affiliation":[{"name":"Instytut Medycyny Doswiadczalnej i Klinicznej, Polska Akademia Nauk, Warszawa, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tadeusz","family":"Kulikowski","sequence":"additional","affiliation":[{"name":"Instytut Biochemii i Biofizyki, Polska Akademia Nauk, Warszawa, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Borowski","sequence":"additional","affiliation":[{"name":"Bernhard-Nocht-Institut f\u00fcr Tropenmedizin, Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2005,10,1]]},"reference":[{"key":"bibr1-095632020501600504","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1033.2003.03540.x"},{"key":"bibr2-095632020501600504","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.7.3220-3229.2001"},{"key":"bibr3-095632020501600504","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"bibr4-095632020501600504","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.73.8.6257-6264.1999"},{"key":"bibr5-095632020501600504","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.24.15045"},{"key":"bibr6-095632020501600504","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.72.8.6758-6769.1998"},{"key":"bibr7-095632020501600504","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.67.3.1385-1395.1993"},{"key":"bibr8-095632020501600504","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1996.1225"},{"key":"bibr9-095632020501600504","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-59605-6"},{"key":"bibr10-095632020501600504","volume-title":"Gel 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