{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T20:37:15Z","timestamp":1777927035390,"version":"3.51.4"},"reference-count":16,"publisher":"SAGE Publications","issue":"8","license":[{"start":{"date-parts":[[2013,12,11]],"date-time":"2013-12-11T00:00:00Z","timestamp":1386720000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Int J STD AIDS"],"published-print":{"date-parts":[[2014,7]]},"abstract":"<jats:p>Summary<\/jats:p>\n                  <jats:p>\n                    Neurosyphilis is a difficult clinical stage of syphilis as there is no ideal method for diagnosis and workup requires lumbar puncture which may sometimes provide ambiguous results especially in HIV co-infected patients. Enzyme immunoassay is a widely used screening test for syphilis in serum, but its test performance was not well studied in cerebrospinal fluid. To examine the diagnostic performance of cerebrospinal fluid-enzyme immunoassay (CSF-EIA) in neurosyphilis, we conducted a prospective study for two years. All consecutive patients admitted for workup of neurosyphilis under the Social Hygiene Service, in Hong Kong, were included. Laboratory tests on CSF included several serological tests, CSF cell count, and protein. Forty-five patients were prospectively recruited, of which 29 people were living with HIV \/ AIDS. Using diagnostic case definition standard stipulated in the IUSTI 2008 guidelines, 17 patients satisfied the diagnosis of neurosyphilis. The CSF-EIA test provided 100% in both sensitivity and negative predictive value; its specificity was 46.4% (13\/28, 95% CI 31.8\u201361%). Specificity improved to 80.8% (95% CI: 68.4\u201393.2%) with optical density cut-off value at 1.4 for cases with CSF red cell counts &lt;600\/mm\n                    <jats:sup>3<\/jats:sup>\n                    . This is the first study on use of CSF-EIA in neurosyphilis. CSF-EIA showed high sensitivity and high negative predictive value in the study population and the presence of CSF red cells &lt; 600\/mm\n                    <jats:sup>3<\/jats:sup>\n                    might not affect its accuracy.\n                  <\/jats:p>","DOI":"10.1177\/0956462413515452","type":"journal-article","created":{"date-parts":[[2013,12,11]],"date-time":"2013-12-11T20:16:36Z","timestamp":1386792996000},"page":"571-578","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":7,"title":["Use of cerebrospinal fluid enzyme immunoassay for diagnosis of neurosyphilis"],"prefix":"10.1177","volume":"25","author":[{"given":"Yung","family":"Chan","sequence":"first","affiliation":[{"name":"Social Hygiene Service, Centre for Health Protection, Department of Health, Hong Kong Special Administrative Region, Hong Kong"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kwok-Hung","family":"Yeung","sequence":"additional","affiliation":[{"name":"Social Hygiene Service, Centre for Health Protection, Department of Health, Hong Kong Special Administrative Region, Hong Kong"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hing-Fung","family":"Ho","sequence":"additional","affiliation":[{"name":"Social Hygiene Service, Centre for Health Protection, Department of Health, Hong Kong Special Administrative Region, Hong Kong"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"King-Man","family":"Ho","sequence":"additional","affiliation":[{"name":"Social Hygiene Service, Centre for Health Protection, Department of Health, Hong Kong Special Administrative Region, Hong Kong"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edman","family":"Tin-Keung Lam","sequence":"additional","affiliation":[{"name":"Public Health Laboratory Centre, Centre for Health Protection, Department of Health, Hong Kong Special Administrative Region, Hong Kong"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wai-Lin","family":"Leung","sequence":"additional","affiliation":[{"name":"Public Health Laboratory Centre, Centre for Health Protection, Department of Health, Hong Kong Special Administrative Region, Hong Kong"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kai-Man","family":"Kam","sequence":"additional","affiliation":[{"name":"Stanley Ho Centre for Emerging Infectious Diseases, School of Public Health and Primary care, Faculty of Medicine, Chinese University of Hong Kong, Hong Kong"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2013,12,11]]},"reference":[{"key":"bibr1-0956462413515452","doi-asserted-by":"publisher","DOI":"10.1258\/ijsa.2008.008279"},{"key":"bibr2-0956462413515452","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.12.2.187"},{"key":"bibr3-0956462413515452","doi-asserted-by":"publisher","DOI":"10.1258\/ijsa.2008.008510"},{"key":"bibr4-0956462413515452","doi-asserted-by":"publisher","DOI":"10.1258\/0956462001915750"},{"key":"bibr5-0956462413515452","first-page":"1","volume":"59","author":"Workowski KA","year":"2010","journal-title":"MMWR Recommen Rep"},{"key":"bibr6-0956462413515452","doi-asserted-by":"publisher","DOI":"10.1111\/j.1468-3083.2008.03021.x"},{"key":"bibr7-0956462413515452","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0b013e31824c0e62"},{"key":"bibr8-0956462413515452","unstructured":"Abbott. ICE* Syphilis Enzyme immunoassay for the detection of antibodies against Treponema pallidum. [Internet]: Abbott Murex, http:\/\/nearmedic.ru\/upload\/files\/5_Murex_ICE_Syphilis.pdf (2007, accessed 18 January 2013)."},{"key":"bibr9-0956462413515452","unstructured":"Ho KK. The use of recombinant EIA as a serological screening test for syphilis in HIV infection in HK [Dissertation for Dermatology and venereology, Hong Kong College of Physicians], 2002."},{"key":"bibr10-0956462413515452","first-page":"158","volume":"3","author":"Egglestone SI","year":"2000","journal-title":"Commun Dis Public Health"},{"key":"bibr11-0956462413515452","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.4.913-917.1998"},{"key":"bibr12-0956462413515452","volume-title":"Sexually transmitted diseases","author":"Holmes KK","year":"2008","edition":"4"},{"key":"bibr13-0956462413515452","first-page":"34","volume":"34","author":"Danbolt N","year":"1954","journal-title":"Acta Derm Venereol"},{"key":"bibr14-0956462413515452","doi-asserted-by":"publisher","DOI":"10.2340\/00015555-0092"},{"key":"bibr15-0956462413515452","doi-asserted-by":"publisher","DOI":"10.1097\/01.olq.0000230481.28936.e5"},{"key":"bibr16-0956462413515452","first-page":"1103","volume":"68","author":"Seehusen DA","year":"2003","journal-title":"Am Fam Physician"}],"container-title":["International Journal of STD &amp; AIDS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0956462413515452","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/0956462413515452","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0956462413515452","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T22:33:09Z","timestamp":1777674789000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/0956462413515452"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,12,11]]},"references-count":16,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2014,7]]}},"alternative-id":["10.1177\/0956462413515452"],"URL":"https:\/\/doi.org\/10.1177\/0956462413515452","relation":{},"ISSN":["0956-4624","1758-1052"],"issn-type":[{"value":"0956-4624","type":"print"},{"value":"1758-1052","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,12,11]]}}}