{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T20:37:34Z","timestamp":1777927054288,"version":"3.51.4"},"reference-count":13,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2014,5,8]],"date-time":"2014-05-08T00:00:00Z","timestamp":1399507200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Int J STD AIDS"],"published-print":{"date-parts":[[2015,1]]},"abstract":"<jats:p>Nucleic acid amplification tests (NAATs) are the most sensitive method for diagnosing chlamydia and gonorrhoea. We use the COBAS 4800 CT\/NG combined assay (Roche Molecular Diagnostics, CA, USA), and whilst the majority of samples yield definitive results, a small proportion are reported as indeterminate. In these instances, it is usual practice to request repeat samples which delays diagnosis. This audit was twofold: first to establish the proportion of indeterminate results with current NAAT testing requiring re-sampling. Second, to determine whether a second NAAT such as Cepheid GeneXpert CT\/NG assay (Cepheid, CA, USA) could be used on initial indeterminate samples to resolve indeterminate results, therefore reducing need for repeat sampling. During 2012, 144\/21,931 (0.66%) samples were indeterminate for Neisseria gonorrhoeae, Chlamydia trachomatis or both, and a repeat sample was received in only 51.77% of patients with final results being delayed for more than 24\u2009h. Over the next six months, there were 77\/9472 (0.81%) indeterminate results. After an evaluation and introduction of the Cepheid assay, the number of indeterminate results fell to 9 (0.10%). Thus, use of the Cepheid assay significantly reduced indeterminate results, reduced reliance on a repeat sampling and significantly improved turnaround time, laboratory workflow and patient experience.<\/jats:p>","DOI":"10.1177\/0956462414531938","type":"journal-article","created":{"date-parts":[[2014,5,9]],"date-time":"2014-05-09T03:34:22Z","timestamp":1399606462000},"page":"65-67","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":7,"title":["Value of repeat testing using Cepheid GeneXpert CT\/NG for indeterminate PCR results when diagnosing\n                    <i>Chlamydia trachomatis<\/i>\n                    and\n                    <i>Neisseria gonorrhoeae<\/i>"],"prefix":"10.1177","volume":"26","author":[{"given":"Benjamin J","family":"Parcell","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology, Ninewells Hospital and Medical School, NHS Tayside, Dundee, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lasantha","family":"Ratnayake","sequence":"additional","affiliation":[{"name":"James Paget University Hospitals NHS Foundation Trust, Norfolk, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Geraldine","family":"Kaminski","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Ninewells Hospital and Medical School, NHS Tayside, Dundee, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William J","family":"Olver","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Ninewells Hospital and Medical School, NHS Tayside, Dundee, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David L","family":"Yirrell","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Ninewells Hospital and Medical School, NHS Tayside, Dundee, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2014,5,8]]},"reference":[{"key":"bibr1-0956462414531938","unstructured":"SIGN. Management of genital Chlamydia trachomatis infection. A national clinical guideline, Edinburgh: SIGN, 2009, pp. 109\u2013109."},{"key":"bibr2-0956462414531938","volume-title":"Chlamydia trachomatis UK testing guidelines","author":"BASHH","year":"2010"},{"key":"bibr3-0956462414531938","volume-title":"UK National guideline for gonorrhoea testing. Clinical effectiveness group","author":"BASHH","year":"2012"},{"key":"bibr4-0956462414531938","volume-title":"UK standards for microbiology investigations. Detection of Neisseria gonorrhoeae using molecular methods","author":"HPA","year":"2012"},{"key":"bibr5-0956462414531938","volume-title":"Guidance for gonorrhoea testing in England and Wales","author":"BASHH, HPA","year":"2010"},{"key":"bibr6-0956462414531938","volume-title":"Guidance on the use of molecular testing for Neisseria gonorrhoeae in diagnostic laboratories 2011. Molecular testing for gonorrhoea working group","author":"Eastick K","year":"2012"},{"key":"bibr7-0956462414531938","volume-title":"Chlamydia trachomatis infection \u2013 testing by nucleic acid amplification tests (NAATS)","author":"UK Standards for Microbiology Investigations","year":"2013"},{"key":"bibr8-0956462414531938","unstructured":"Personal communication with Cepheid EU Support (support@cepheideurope.com), 2014."},{"key":"bibr9-0956462414531938","doi-asserted-by":"publisher","DOI":"10.1136\/sextrans-2011-050075"},{"key":"bibr10-0956462414531938","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2012.05384.x"},{"key":"bibr11-0956462414531938","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01217-11"},{"key":"bibr12-0956462414531938","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00806-13"},{"key":"bibr13-0956462414531938","unstructured":"NHS North West. Feeling unwell fact sheet, NHS http:\/\/www.northwest.nhs.uk\/document_uploads\/Choose%20Well\/A4_feeling_unwell_posters.pdf (accessed 27 February 2014)."}],"container-title":["International Journal of STD &amp; AIDS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0956462414531938","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/0956462414531938","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0956462414531938","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T22:33:47Z","timestamp":1777674827000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/0956462414531938"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,5,8]]},"references-count":13,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2015,1]]}},"alternative-id":["10.1177\/0956462414531938"],"URL":"https:\/\/doi.org\/10.1177\/0956462414531938","relation":{},"ISSN":["0956-4624","1758-1052"],"issn-type":[{"value":"0956-4624","type":"print"},{"value":"1758-1052","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,5,8]]}}}