{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T04:56:02Z","timestamp":1783140962748,"version":"3.54.6"},"reference-count":13,"publisher":"SAGE Publications","issue":"9","license":[{"start":{"date-parts":[[2018,4,9]],"date-time":"2018-04-09T00:00:00Z","timestamp":1523232000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100005878","name":"Terveyden Tutkimuksen Toimikunta","doi-asserted-by":"publisher","award":["1285975"],"award-info":[{"award-number":["1285975"]}],"id":[{"id":"10.13039\/501100005878","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008376","name":"Helsingin ja Uudenmaan Sairaanhoitopiiri","doi-asserted-by":"publisher","award":["EVO 2014117"],"award-info":[{"award-number":["EVO 2014117"]}],"id":[{"id":"10.13039\/100008376","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008376","name":"Helsingin ja Uudenmaan Sairaanhoitopiiri","doi-asserted-by":"publisher","award":["EVO 2015318"],"award-info":[{"award-number":["EVO 2015318"]}],"id":[{"id":"10.13039\/100008376","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008376","name":"Helsingin ja Uudenmaan Sairaanhoitopiiri","doi-asserted-by":"publisher","award":["V9300M2016"],"award-info":[{"award-number":["V9300M2016"]}],"id":[{"id":"10.13039\/100008376","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Int J STD AIDS"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:p>The aim was to examine the prevalence of Mycoplasma genitalium and to determine the prevalence of mutations leading to resistance to macrolides and fluoroquinolones in a sexually transmitted infection clinic setting in Finland, and as a service evaluation, to validate the performance of a commercial Aptima\u00ae Mycoplasma genitalium assay. Urogenital samples were studied for M. genitalium with an automated commercial Aptima\u00ae Mycoplasma genitalium assay on the Panther\u00ae system (Hologic), and with an in-house real-time polymerase chain reaction (PCR) (mgpB). Positive specimens were further studied for mutations associated with macrolide resistance within the 23S rRNA gene and the known quinolone resistance-determining regions within genes gyrA, gyrB and parC. Altogether 17\/303 (5.6%) of samples contained M. genitalium by either test. Two of the samples positive by the Aptima assay were not detected by the in-house PCR assay, although the internal control (beta-globin gene) was amplified. The Aptima assay gave an invalid result for five samples, all of which were negative by the in-house PCR. Mutations resulting in macrolide resistance were detected in 30.8% of M. genitalium-positive specimens. Prevalence of M. genitalium infections in the specimens tested is similar to that in other parts of Europe, 5.6%. The Aptima\u00ae Mycoplasma genitalium assay detected slightly more positives than the in-house PCR assay. Mutations resulting in macrolide resistance were common in M. genitalium and detection of these mutations is recommended in diagnostic laboratories to assist in selection of treatment.<\/jats:p>","DOI":"10.1177\/0956462418764482","type":"journal-article","created":{"date-parts":[[2018,4,10]],"date-time":"2018-04-10T01:33:07Z","timestamp":1523323987000},"page":"904-907","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":24,"title":["Prevalence of\n                    <i>Mycoplasma genitalium<\/i>\n                    and mutations associated with macrolide and fluoroquinolone resistance in Finland"],"prefix":"10.1177","volume":"29","author":[{"given":"Kati","family":"Hokynar","sequence":"first","affiliation":[{"name":"Department of Virology, University of Helsinki, Helsinki, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eija","family":"Hiltunen-Back","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Helsinki University Central Hospital, Helsinki, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Mannonen","sequence":"additional","affiliation":[{"name":"Virology and Immunology, Helsinki University Hospital, Helsinki, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7413-6793","authenticated-orcid":false,"given":"Mirja","family":"Puolakkainen","sequence":"additional","affiliation":[{"name":"Department of Virology, University of Helsinki, Helsinki, Finland"},{"name":"Virology and Immunology, Helsinki University Hospital, Helsinki, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2018,4,9]]},"reference":[{"key":"bibr1-0956462418764482","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(17)30364-X"},{"key":"bibr2-0956462418764482","doi-asserted-by":"publisher","DOI":"10.1093\/ije\/dyv194"},{"key":"bibr3-0956462418764482","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00006-11"},{"key":"bibr4-0956462418764482","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jix132"},{"key":"bibr5-0956462418764482","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00495-13"},{"key":"bibr6-0956462418764482","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01956-12"},{"key":"bibr7-0956462418764482","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cit752"},{"key":"bibr8-0956462418764482","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2016.07.005"},{"key":"bibr9-0956462418764482","doi-asserted-by":"publisher","DOI":"10.1111\/jdv.13849"},{"key":"bibr10-0956462418764482","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.2.683-692.2004"},{"key":"bibr11-0956462418764482","doi-asserted-by":"publisher","DOI":"10.1186\/s40064-015-1457-x"},{"key":"bibr12-0956462418764482","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2017.09.006"},{"key":"bibr13-0956462418764482","doi-asserted-by":"publisher","DOI":"10.2340\/00015555-2314"}],"container-title":["International Journal of STD &amp; AIDS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0956462418764482","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/0956462418764482","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0956462418764482","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T22:38:09Z","timestamp":1777675089000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/0956462418764482"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,4,9]]},"references-count":13,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2018,8]]}},"alternative-id":["10.1177\/0956462418764482"],"URL":"https:\/\/doi.org\/10.1177\/0956462418764482","relation":{},"ISSN":["0956-4624","1758-1052"],"issn-type":[{"value":"0956-4624","type":"print"},{"value":"1758-1052","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,4,9]]}}}