{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T20:40:32Z","timestamp":1777927232799,"version":"3.51.4"},"reference-count":37,"publisher":"SAGE Publications","issue":"13","license":[{"start":{"date-parts":[[2018,7,4]],"date-time":"2018-07-04T00:00:00Z","timestamp":1530662400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"US Military HIV Research Program","award":["W81XWH-07-2-0067"],"award-info":[{"award-number":["W81XWH-07-2-0067"]}]},{"name":"National Institute of Health","award":["R01MH099001-01"],"award-info":[{"award-number":["R01MH099001-01"]}]},{"name":"National Institute of Health","award":["U-01068613"],"award-info":[{"award-number":["U-01068613"]}]},{"DOI":"10.13039\/100000002","name":"US National Institutes of Health","doi-asserted-by":"crossref","award":["R01AI120913"],"award-info":[{"award-number":["R01AI120913"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc","award":["W81XWH-11-2-0174"],"award-info":[{"award-number":["W81XWH-11-2-0174"]}]},{"name":"President's Emergency Plan for AIDS Relief","award":["U2G IPS000651"],"award-info":[{"award-number":["U2G IPS000651"]}]},{"name":"HHS\/Centers for Disease Control and Prevention (CDC), Global AIDS Program with IHVN","award":["U54EB007958"],"award-info":[{"award-number":["U54EB007958"]}]},{"name":"Fogarty AITRP","award":["D43TW01041"],"award-info":[{"award-number":["D43TW01041"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Int J STD AIDS"],"published-print":{"date-parts":[[2018,11]]},"abstract":"<jats:p>Antimicrobial-resistant Neisseria gonorrhoeae (NG) is a global public health issue that threatens effectiveness of current treatments of NG. Increased use of nucleic acid amplification tests (NAATs) in lieu of cultures makes obtaining clinical isolates for susceptibility testing difficult and samples collected in commercial transport buffer for NAATs do not preserve viable organism, while molecular methods of assessing antibiotic susceptibility do not require viable organism. We evaluated 243 NG-positive samples in Aptima transport media including urine, oral, and rectal swabs from Nigerian men who have sex with men for markers to penicillinase-producing NG, ciprofloxacin ( GyrA and ParC mutations), and extended spectrum cephalosporins (ESCs, PenA mosaic [allele X], PonA, mtrR, PorB mutations) by real-time PCR. NG DNA was recovered in 75% (183\/243) of samples. Of these, 93% (171\/183) were positive for at least one resistance marker. We observed a prevalence of dual resistance markers to penicillin and ciprofloxacin at 46.2% (79\/171). Six percent of samples (10\/171) tested positive for the PenA mosaic (allele X) ESC marker. These data indicate that antibiotic-resistant NG is common in Nigeria. Laboratory and clinical capacity building in Nigeria should include development of methods to culture NG and determine antimicrobial susceptibility.<\/jats:p>","DOI":"10.1177\/0956462418780050","type":"journal-article","created":{"date-parts":[[2018,7,4]],"date-time":"2018-07-04T04:47:06Z","timestamp":1530679626000},"page":"1273-1281","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":8,"title":["Molecular screening for\n                    <i>Neisseria gonorrhoeae<\/i>\n                    antimicrobial resistance markers in Nigerian men who have sex with men and transgender women"],"prefix":"10.1177","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3926-7784","authenticated-orcid":false,"given":"Justin","family":"Hardick","sequence":"first","affiliation":[{"name":"Johns Hopkins University School of Medicine, Baltimore, MD, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Trevor A","family":"Crowell","sequence":"additional","affiliation":[{"name":"U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA"},{"name":"Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kara","family":"Lombardi","sequence":"additional","affiliation":[{"name":"U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA"},{"name":"Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akindiran","family":"Akintunde","sequence":"additional","affiliation":[{"name":"U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA"},{"name":"Henry M. Jackson Foundation for the Advancement of Military Medicine, Abuja, Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sunday","family":"Odeyemi","sequence":"additional","affiliation":[{"name":"U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA"},{"name":"Henry M. Jackson Foundation for the Advancement of Military Medicine, Abuja, Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew","family":"Ivo","sequence":"additional","affiliation":[{"name":"U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA"},{"name":"Henry M. Jackson Foundation for the Advancement of Military Medicine, Abuja, Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"George","family":"Eluwa","sequence":"additional","affiliation":[{"name":"Population Council Nigeria, Abuja, Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean","family":"Njab","sequence":"additional","affiliation":[{"name":"Population Council Nigeria, Abuja, Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan D","family":"Baral","sequence":"additional","affiliation":[{"name":"Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rebecca G","family":"Nowak","sequence":"additional","affiliation":[{"name":"Institute of Human Virology, University of Maryland, Baltimore, MD, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas C","family":"Quinn","sequence":"additional","affiliation":[{"name":"Johns Hopkins University School of Medicine, Baltimore, MD, USA"},{"name":"Division of Intramural Research, NIAID, NIH, Bethesda, MD, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kent","family":"Barbian","sequence":"additional","affiliation":[{"name":"Rocky Mountain National Laboratory (RML), Hamilton, MT, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah","family":"Anzick","sequence":"additional","affiliation":[{"name":"Rocky Mountain National Laboratory (RML), Hamilton, MT, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sylvia","family":"Adebajo","sequence":"additional","affiliation":[{"name":"Population Council Nigeria, Abuja, Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manhattan E","family":"Charurat","sequence":"additional","affiliation":[{"name":"Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julie","family":"Ake","sequence":"additional","affiliation":[{"name":"U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charlotte A","family":"Gaydos","sequence":"additional","affiliation":[{"name":"Johns Hopkins University School of Medicine, Baltimore, MD, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2018,7,4]]},"reference":[{"key":"bibr1-0956462418780050","doi-asserted-by":"publisher","DOI":"10.15585\/mmwr.ss6507a1"},{"key":"bibr2-0956462418780050","doi-asserted-by":"crossref","unstructured":"Costa Louren\u00e7o APRD, Barros Dos Santos KT, Moreira BM, et\u00a0al. Antimicrobial resistance in\n                      Neisseria gonorrhoeae\n                      : history, molecular mechanisms and epidemiological aspects of an emerging global threat.\n                      Braz J Microbiol\n                      2017; 48: 617\u2013628.","DOI":"10.1016\/j.bjm.2017.06.001"},{"key":"bibr3-0956462418780050","doi-asserted-by":"crossref","first-page":"e0143304","DOI":"10.1371\/journal.pone.0143304","volume":"12","author":"Newman L","year":"2015","journal-title":"PLoS One"},{"key":"bibr4-0956462418780050","unstructured":"World Health Organization (WHO) guidelines for the treatment of\n                      Neisseria gonorrhoeae\n                      . Geneva: World Health Organization, 2016."},{"key":"bibr5-0956462418780050","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-015-1029-2"},{"key":"bibr6-0956462418780050","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2011.06215.x"},{"key":"bibr7-0956462418780050","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1002344"},{"key":"bibr8-0956462418780050","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cix485"},{"key":"bibr9-0956462418780050","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cix492"},{"key":"bibr10-0956462418780050","unstructured":"World Health Organization (WHO), Department of Reproductive Health and Research. Global action plan to control the spread and impact of antimicrobial resistance in\n                      Neisseria gonorrhoeae\n                      . \nGeneva: \nWHO, p.136, http:\/\/www.who.int\/reproductivehealth\/publications\/rtis\/9789241503501 (2012, accessed 13 September 2017)."},{"key":"bibr11-0956462418780050","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dku396"},{"key":"bibr12-0956462418780050","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-158-5-201303050-00004"},{"key":"bibr13-0956462418780050","doi-asserted-by":"publisher","DOI":"10.1016\/j.gheart.2014.08.009"},{"key":"bibr14-0956462418780050","doi-asserted-by":"crossref","unstructured":"Latif AS, Gwanzura L, Machiha A, et\u00a0al. Antimicrobial susceptibility in\n                      Neisseria gonorrhoeae\n                      isolates from five sentinel surveillance sites in Zimbabwe, 2015\u20132016.\n                      Sex Transm Infect\n                      2018; 94: 62\u201366.","DOI":"10.1136\/sextrans-2016-053090"},{"key":"bibr15-0956462418780050","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0000000000000204"},{"key":"bibr16-0956462418780050","doi-asserted-by":"publisher","DOI":"10.1136\/sextrans-2012-050907"},{"key":"bibr17-0956462418780050","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2014.10.020"},{"key":"bibr18-0956462418780050","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.7.3281-3283.2004"},{"key":"bibr19-0956462418780050","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02024-10"},{"key":"bibr20-0956462418780050","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmoldx.2012.08.005"},{"key":"bibr21-0956462418780050","doi-asserted-by":"publisher","DOI":"10.1080\/14737159.2017.1360137"},{"key":"bibr22-0956462418780050","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01800-07"},{"key":"bibr23-0956462418780050","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00493-15"},{"key":"bibr24-0956462418780050","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro3217"},{"key":"bibr25-0956462418780050","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkt501"},{"key":"bibr26-0956462418780050","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkv432"},{"key":"bibr27-0956462418780050","doi-asserted-by":"crossref","first-page":"e015447","DOI":"10.1136\/bmjopen-2016-015447","volume":"14","author":"Turner KM","year":"2017","journal-title":"BMJ Open"},{"key":"bibr28-0956462418780050","doi-asserted-by":"publisher","DOI":"10.7448\/IAS.19.1.21270"},{"key":"bibr29-0956462418780050","doi-asserted-by":"publisher","DOI":"10.1080\/00313020500254552"},{"key":"bibr30-0956462418780050","doi-asserted-by":"publisher","DOI":"10.1097\/QAI.0000000000000439"},{"key":"bibr31-0956462418780050","first-page":"13","author":"Ali S","year":"2016","journal-title":"BMC Res Notes"},{"key":"bibr32-0956462418780050","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0000000000000099"},{"key":"bibr33-0956462418780050","doi-asserted-by":"publisher","DOI":"10.7205\/MILMED-D-13-00418"},{"key":"bibr34-0956462418780050","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0b013e318248a85f"},{"key":"bibr35-0956462418780050","first-page":"72","volume":"10","author":"Banwat EB","year":"2001","journal-title":"Niger J Med"},{"key":"bibr36-0956462418780050","doi-asserted-by":"publisher","DOI":"10.1080\/10158782.2012.11441509"},{"key":"bibr37-0956462418780050","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkx067"}],"container-title":["International Journal of STD &amp; AIDS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0956462418780050","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/0956462418780050","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0956462418780050","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T22:38:27Z","timestamp":1777675107000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/0956462418780050"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,7,4]]},"references-count":37,"journal-issue":{"issue":"13","published-print":{"date-parts":[[2018,11]]}},"alternative-id":["10.1177\/0956462418780050"],"URL":"https:\/\/doi.org\/10.1177\/0956462418780050","relation":{},"ISSN":["0956-4624","1758-1052"],"issn-type":[{"value":"0956-4624","type":"print"},{"value":"1758-1052","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,7,4]]}}}