{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T20:43:43Z","timestamp":1777927423977,"version":"3.51.4"},"reference-count":26,"publisher":"SAGE Publications","issue":"14","license":[{"start":{"date-parts":[[2022,10,11]],"date-time":"2022-10-11T00:00:00Z","timestamp":1665446400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100001321","name":"National Research Foundation","doi-asserted-by":"crossref","award":["112555"],"award-info":[{"award-number":["112555"]}],"id":[{"id":"10.13039\/501100001321","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Int J STD AIDS"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Macrolide resistance in Mycoplasma genitalium ( M. genitalium) is increasing as a result of the widespread use of azithromycin in the treatment of sexually transmitted infections (STIs). To date, there are few published studies on macrolide resistance patterns in South African pregnant women. This study now contributes to the growing body of knowledge.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      This study included 385 pregnant women living with HIV. Vaginal swabs were collected from consenting pregnant women and used for the detection of M. genitalium using the TaqMan assay . Macrolide resistance-associated mutations in the 23S rRNA gene were determined for all samples that tested positive for M. genitalium using the Allplex\n                      <jats:sup>TM<\/jats:sup>\n                      MG &amp; AziR assay (Seegene) which allows for the simultaneous detection and identification of M. genitalium and six mutations ( A2058C, A2058G, A2058T, A2059C, A2059G and A2059T) responsible for azithromycin resistance. The correlation between the TaqMan assay and Allplex\n                      <jats:sup>TM<\/jats:sup>\n                      MG &amp; AziR assay (Seegene) for the detection of M. genitalium was also performed in a subset of 121 samples.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Of the 385 samples tested in this study, 14 samples were positive for M. genitalium estimating a prevalence of 3.6%. The same 14 samples also tested positive on the Allplex\n                      <jats:sup>TM<\/jats:sup>\n                      assay indicating a good correlation between the TaqMan Assay and the Allplex\n                      <jats:sup>TM<\/jats:sup>\n                      . Of the 14 positive samples, one sample carried a mutation at position A2059G denoting macrolide resistance in this pathogen. Mutations in the other regions of the 23S rRNA were not detected. All assay controls used in the mutation scanning produced the desired results showing the validity of the assay.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>In this study, macrolide resistance in M. genitalium was detected. Despite the low prevalence of resistance determinants ongoing antimicrobial resistance surveillance is vital considering that azithromycin is used in the syndromic management for the treatment of vaginal discharge syndrome.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/09564624221129412","type":"journal-article","created":{"date-parts":[[2022,10,11]],"date-time":"2022-10-11T05:39:43Z","timestamp":1665466783000},"page":"1174-1182","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":4,"title":["Low prevalence of macrolide resistance in\n                    <i>Mycoplasma genitalium<\/i>\n                    infections in a cohort of pregnant women living with human immunodeficiency virus"],"prefix":"10.1177","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1573-4520","authenticated-orcid":false,"given":"Bongekile","family":"Ngobese","sequence":"first","affiliation":[{"name":"School of Clinical Medicine Laboratory, College of Health Science, Nelson R Mandela School of Medicine, University of KwaZulu-Natal, Durban, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Khine","family":"Swe Swe-Han","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, School of Laboratory Medicine and Medical Sciences, College of Health Sciences, Nelson R Mandela School of Medicine, University of KwaZulu-Natal, Durban, South Africa"},{"name":"Department of Microbiology, National Health Laboratory Services, KwaZulu-Natal Academic Complex, Inkosi Albert Luthuli Central Hospital, Durban, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Partson","family":"Tinarwo","sequence":"additional","affiliation":[{"name":"Department of Biostatistics, Nelson R Mandela School of Medicine, University of KwaZulu-Natal, Durban, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nathlee S","family":"Abbai","sequence":"additional","affiliation":[{"name":"School of Clinical Medicine Laboratory, College of Health Science, Nelson R Mandela School of Medicine, University of KwaZulu-Natal, Durban, South 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