{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T20:43:38Z","timestamp":1777927418637,"version":"3.51.4"},"reference-count":31,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2023,1,13]],"date-time":"2023-01-13T00:00:00Z","timestamp":1673568000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Team Klausner Saving Lives"},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI099727-01"],"award-info":[{"award-number":["R01AI099727-01"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Int J STD AIDS"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The increasing prevalence of drug-resistant Neisseria gonorrhoeae (NG) infections has caused great concern. Ciprofloxacin remains the empiric antimicrobial recommended to treat NG infections in Peru disregarding the susceptibility profile of circulating NG strains. We report the prevalence of individuals infected with NG strains presenting mutations in the gyrA gene that confers ciprofloxacin resistance.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We conducted a descriptive study assessing extragenital swab samples collected from a cohort of men who have sex with men and transgender women in Lima, Peru. Anal and pharyngeal NG positive swabs for Aptima Combo 2 assay (Hologic Inc., USA) were used for DNA extraction. We performed TaqMan real time PCR assays to detect a point mutation at codon Ser91 of the gyrase A ( gyrA) gene.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>From 156 individuals who had at least one positive sample for NG reported by the Aptima assay, 80 individuals had at least one amplified DNA for the gyrA gene. We found that 67 of them (84.0%) were infected with a gyrA-mutated NG strain at the Ser91 codon.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>We report a high prevalence of gyrA mutation conferring ciprofloxacin resistance among individuals with extragenital NG infection. Empirical treatment of NG needs to be urgently updated in Peru in concordance with international guidelines.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/09564624221147326","type":"journal-article","created":{"date-parts":[[2023,1,13]],"date-time":"2023-01-13T09:59:53Z","timestamp":1673603993000},"page":"245-250","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["Extra-genital\n                    <i>Neisseria gonorrhoeae<\/i>\n                    infections with genetic mutations conferring ciprofloxacin resistance among men who have sex with men and transgender women in Lima, Peru"],"prefix":"10.1177","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2647-2933","authenticated-orcid":false,"given":"Jazmin","family":"Qquellon","sequence":"first","affiliation":[{"name":"Center for Interdisciplinary Studies in Sexuality, AIDS and Society, Universidad Peruana Cayetano Heredia, Lima, Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silver K","family":"Vargas","sequence":"additional","affiliation":[{"name":"Center for Interdisciplinary Studies in Sexuality, AIDS and Society, Universidad Peruana Cayetano Heredia, Lima, Peru"},{"name":"School of Public Health and Administration, Universidad Peruana Cayetano Heredia, Lima, Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Eguiluz","sequence":"additional","affiliation":[{"name":"Center for Interdisciplinary Studies in Sexuality, AIDS and Society, Universidad Peruana Cayetano Heredia, Lima, Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesca","family":"Vasquez","sequence":"additional","affiliation":[{"name":"Center for Interdisciplinary Studies in Sexuality, AIDS and Society, Universidad Peruana Cayetano Heredia, Lima, Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Durand","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Tropical \u201cAlexander Von Humboldt\u201d, Universidad Peruana Cayetano Heredia, Lima, Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lao-Tzu","family":"Allan-Blitz","sequence":"additional","affiliation":[{"name":"Division of Global Health Equity, Department of Medicine, Brigham and Women\u2019s Hospital, Boston, MA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kelika A","family":"Konda","sequence":"additional","affiliation":[{"name":"Center for Interdisciplinary Studies in Sexuality, AIDS and Society, Universidad Peruana Cayetano Heredia, Lima, Peru"},{"name":"Division of Infectious Diseases, David Geffen School of Medicine, University of California, Los Angeles, CA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Theresa J","family":"Ochoa","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Tropical \u201cAlexander Von Humboldt\u201d, Universidad Peruana Cayetano Heredia, Lima, Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos F","family":"Caceres","sequence":"additional","affiliation":[{"name":"Center for Interdisciplinary Studies in Sexuality, AIDS and Society, Universidad Peruana Cayetano Heredia, Lima, Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6922-7364","authenticated-orcid":false,"given":"Jeffrey D","family":"Klausner","sequence":"additional","affiliation":[{"name":"Department of Population and Public Health Sciences, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2023,1,13]]},"reference":[{"key":"bibr1-09564624221147326","volume-title":"Global progress report on HIV, viral hepatitis and sexually transmitted infections","author":"World Health Organization","year":"2021"},{"key":"bibr2-09564624221147326","doi-asserted-by":"publisher","DOI":"10.1071\/SH19061"},{"key":"bibr3-09564624221147326","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(12)70144-5"},{"key":"bibr4-09564624221147326","doi-asserted-by":"publisher","DOI":"10.1136\/bmjopen-2015-008245"},{"key":"bibr5-09564624221147326","volume-title":"Multi-drug resistant gonorrhoea","author":"World Health Organization","year":"2021"},{"key":"bibr6-09564624221147326","doi-asserted-by":"publisher","DOI":"10.1016\/j.annemergmed.2007.06.012"},{"key":"bibr7-09564624221147326","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciaa596"},{"key":"bibr8-09564624221147326","unstructured":"Ministerio de Salud del Peru (MINSA). 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