{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T16:44:41Z","timestamp":1792601081935,"version":"4.5.0"},"reference-count":23,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T00:00:00Z","timestamp":1792281600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"NextGenerationEU-MUR PNRR Extended Partnership initiative on Emerging Infectious Diseases","award":["PE00000007, INF-ACT"],"award-info":[{"award-number":["PE00000007, INF-ACT"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Int J STD AIDS"],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Antimicrobial resistance is increasingly challenging the clinical management of\n                      <jats:italic toggle=\"yes\">Mycoplasma genitalium<\/jats:italic>\n                      infections. This study aimed to characterize the genomic diversity, population structure, molecular typing profiles, and distribution of antimicrobial resistance-associated determinants among publicly available\n                      <jats:italic toggle=\"yes\">M. genitalium<\/jats:italic>\n                      genomes.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      All 64\u00a0\n                      <jats:italic toggle=\"yes\">M. genitalium<\/jats:italic>\n                      genomes publicly available in NCBI and PubMLST at the time of data collection were included. Genomes were characterized by\n                      <jats:italic toggle=\"yes\">mgpB<\/jats:italic>\n                      and MG309 molecular typing and screened for mutations associated with macrolide and fluoroquinolone resistance. Phylogenetic relationships were investigated using a recombination-filtered core-genome alignment, and molecular typing and antimicrobial resistance profiles were mapped onto the resulting maximum-likelihood phylogeny.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Substantial molecular diversity was observed, with 29\n                      <jats:italic toggle=\"yes\">mgpB<\/jats:italic>\n                      and 21 MG309 types identified. Macrolide resistance-associated 23S rRNA mutations were detected in 34\/64 (53%) genomes. The fluoroquinolone resistance-associated ParC substitutions S83I and S83\u00a0R were detected in 14 (22%) and one genome, respectively. Relevant GyrA substitutions were less frequent and occurred exclusively in genomes also carrying ParC S83I. Overall, 15\/64 (23%) genomes exhibited a dual-resistance genotype, whereas 19\/64 (30%) carried macrolide resistance-associated mutations without concomitant fluoroquinolone resistance markers. Resistance-associated genotypes occurred across multiple phylogenetically distinct genomic backgrounds, while clusters of closely related isolates sharing molecular typing and resistance profiles were also identified. Conventional\n                      <jats:italic toggle=\"yes\">mgpB<\/jats:italic>\n                      and MG309 typing did not consistently recapitulate whole-genome phylogenetic relationships.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      These findings reveal substantial genomic diversity and a high frequency of resistance-associated determinants among publicly available\n                      <jats:italic toggle=\"yes\">M. genitalium<\/jats:italic>\n                      genomes, supporting the expansion of geographically representative genomic surveillance to monitor the emergence and dissemination of antimicrobial resistance.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/09564624261498927","type":"journal-article","created":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T06:34:46Z","timestamp":1792391686000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Genomic diversity and antimicrobial resistance determinants among publicly available\n                    <i>M<\/i>\n                    <i>ycoplasma genitalium<\/i>\n                    genomes: a comparative phylogenomic analysis"],"prefix":"10.1177","author":[{"given":"Raul","family":"Cetatean","sequence":"first","affiliation":[{"name":"University of Pavia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7253-8799","authenticated-orcid":false,"given":"Claudio","family":"Foschi","sequence":"additional","affiliation":[{"name":"University of Bologna"},{"name":"IRCCS Azienda Ospedaliero-Universitaria di Bologna"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiziana","family":"Lazzarotto","sequence":"additional","affiliation":[{"name":"University of Bologna"},{"name":"IRCCS Azienda Ospedaliero-Universitaria di Bologna"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simone","family":"Ambretti","sequence":"additional","affiliation":[{"name":"University of Bologna"},{"name":"IRCCS Azienda Ospedaliero-Universitaria di Bologna"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,10,18]]},"reference":[{"key":"e_1_3_7_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00006-11"},{"key":"e_1_3_7_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/jdv.17972"},{"key":"e_1_3_7_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.nmni.2026.101747"},{"key":"e_1_3_7_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(20)30154-7"},{"key":"e_1_3_7_6_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1706.101055"},{"key":"e_1_3_7_7_2","doi-asserted-by":"crossref","unstructured":"Fern\u00e1ndez-Huerta M Moure Z Torrecillas M et al. Contribution of ParC -and GyrA-mutations to fluoroquinolone treatment failures in mycoplasma genitalium: a systematized review. Sex Transm Dis 2026; 53: 818-831. https:\/\/doi.org\/10.1097\/OLQ.0000000000002370 Epub ahead of print.","DOI":"10.1097\/OLQ.0000000000002370"},{"key":"e_1_3_7_8_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.904494"},{"key":"e_1_3_7_9_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-017-4399-6"},{"issue":"1","key":"e_1_3_7_10_2","first-page":"001622","article-title":"Novel features of Mycoplasma genitalium genomes identified through Oxford nanopore sequence analysis of isolates from Australia","volume":"12","author":"Huaman JL","year":"2026","unstructured":"Huaman JL, Bradshaw CS, Chua TP, et al. Novel features of Mycoplasma genitalium genomes identified through Oxford nanopore sequence analysis of isolates from Australia. Microb Genom 2026; 12(1): 001622. https:\/\/doi.org\/10.1099\/mgen.0.001622","journal-title":"Microb Genom"},{"key":"e_1_3_7_11_2","doi-asserted-by":"publisher","DOI":"10.12688\/wellcomeopenres.14826.1"},{"key":"e_1_3_7_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw569"},{"key":"e_1_3_7_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/mst010"},{"key":"e_1_3_7_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gku1196"},{"key":"e_1_3_7_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msaa015"},{"key":"e_1_3_7_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4285"},{"key":"e_1_3_7_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msx281"},{"key":"e_1_3_7_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/cpbi.96"},{"key":"e_1_3_7_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00003-06"},{"key":"e_1_3_7_20_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0035593"},{"key":"e_1_3_7_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.lanmic.2024.101047"},{"key":"e_1_3_7_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.03845-23"},{"key":"e_1_3_7_23_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciad057"},{"key":"e_1_3_7_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/aac.00278-22"}],"container-title":["International Journal of STD &amp; AIDS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/09564624261498927","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/09564624261498927","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/09564624261498927","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T16:23:39Z","timestamp":1792599819000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/09564624261498927"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,18]]},"references-count":23,"alternative-id":["10.1177\/09564624261498927"],"URL":"https:\/\/doi.org\/10.1177\/09564624261498927","relation":{},"ISSN":["0956-4624","1758-1052"],"issn-type":[{"value":"0956-4624","type":"print"},{"value":"1758-1052","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,18]]},"article-number":"09564624261498927"}}