{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T20:48:55Z","timestamp":1777927735708,"version":"3.51.4"},"reference-count":23,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[1992,5,1]],"date-time":"1992-05-01T00:00:00Z","timestamp":704678400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int J STD AIDS"],"published-print":{"date-parts":[[1992,5]]},"abstract":"<jats:p>We have compared the efficacy of digoxigenin- and biotin-labelled probes in detecting HPV DNA by in situ hybridization on paraffin-embedded tissue sections of 57 male condyloma-suspect genital lesions. Each biopsy was hybridized with at least three of the following four methods: digoxigenin-labelled HPV DNA probes (Dig-HPV), biotinylated HPV-DNA probes (Bio-HPV), and two commercial methods (ViraType in situ and PathoGene), both based on biotinylated DNA probes. The hybridization products were visualized with colourigenic enzyme substrates.<\/jats:p>\n                  <jats:p>In most biopsies, the 4 methods gave equal results although cross-hybridization was most often found with the low-stringency ViraType method. Dig-HPV 6\/11 probes gave positive results about twice as often as either of the commercial methods. No such difference, however, was found for HPV 16\/18 probes.<\/jats:p>\n                  <jats:p>DNA of any type of HPV 6\/11, 16\/18 or 31\/33\/35 or 51 was detected in 28\/43 (65%) of lesions showing condyloma acuminatum histology but in none of the 14 biopsies with no histological signs of HPV infection. In HPV-positive condylomata with no cellular atypia. HPV 6\/11 was detected in 87% (13\/15), and HPV 16\/18 in 27% (4\/15). In biopsies with cellular atypia, HPV types 6\/11 were detected in 62% (8\/13), HPV types 16\/18 in 46% (6\/13), and HPV types 31\/33\/35 or 51 in 50% (6\/12). In about 50% of the biopsies where at least one hybridization method gave a positive result, either one of the commercial methods gave a negative result. Thus, in situ hybridization using digoxigenin-labelled HPV DNA probes is at least as sensitive a method for HPV detection in paraffin embedded biopsy tissue as in situ hybridization using biotin-labelled probes. Combination of in situ hybridization using digoxigenin-labelled HPV DNA probes with in situ hybridization with biotinylated HPV DNA probes may further improve sensitivity of HPV DNA detection in paraffin-embedded biopsy material.<\/jats:p>","DOI":"10.1177\/095646249200300308","type":"journal-article","created":{"date-parts":[[2017,1,15]],"date-time":"2017-01-15T22:18:47Z","timestamp":1484518727000},"page":"196-203","source":"Crossref","is-referenced-by-count":4,"title":["Comparison of Four\n                    <i>In Situ<\/i>\n                    Hybridization Methods, Based on Digoxigenin- and Biotin-Labelled Probes, in Detecting HPV DNA in Male Condylomata Acuminata"],"prefix":"10.1177","volume":"3","author":[{"given":"J","family":"Lassus","sequence":"first","affiliation":[{"name":"Department of Dermatology and Venereal Diseases, Helsinki University Central Hospital, Helsinki"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K-M","family":"Niemi","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Venereal Diseases, Helsinki University Central Hospital, Helsinki"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Marjam\u00e4ki","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Sciences University of Tampere, Kuopio, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Syrjanen","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Kuopio, Kuopio, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Kartamaa","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Venereal Diseases, Helsinki University Central Hospital, Helsinki"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Lehmus","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Sciences University of Tampere, Kuopio, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K","family":"Krohn","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Sciences University of Tampere, Kuopio, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Ranki","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Venereal Diseases, Helsinki University Central Hospital, Helsinki"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[1992,5,1]]},"reference":[{"key":"bibr1-095646249200300308","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1984.tb01944.x"},{"key":"bibr2-095646249200300308","doi-asserted-by":"publisher","DOI":"10.1038\/321246a0"},{"key":"bibr3-095646249200300308","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.58.1.225-229.1986"},{"key":"bibr4-095646249200300308","doi-asserted-by":"publisher","DOI":"10.1007\/BF00234092"},{"key":"bibr5-095646249200300308","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910320507"},{"key":"bibr6-095646249200300308","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1890320103"},{"key":"bibr7-095646249200300308","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(86)92067-2"},{"key":"bibr8-095646249200300308","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1890160307"},{"key":"bibr9-095646249200300308","first-page":"174","volume":"123","author":"Crum 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