{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T12:16:59Z","timestamp":1777465019626,"version":"3.51.4"},"reference-count":20,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2025,1,22]],"date-time":"2025-01-22T00:00:00Z","timestamp":1737504000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Bio-Medical Materials and Engineering"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Tuberculosis (TB) is a global health challenge from a single infectious agent, Mycobacterium tuberculosis (MTB), and it demands improved diagnostics and therapies.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>This work explored a novel method for detecting MTB by combining nanogold labeling (NGL) technology with silver staining to enhance sensitivity and specificity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Nanogold particles (NGPs) were characterized using ultraviolet absorption spectroscopy (UVAS), and their morphology was observed via transmission electron microscopy (TEM). The silver staining enhancement (SSE) system was optimized for a reaction time of 11\u2005min. Fifty drug-resistant tuberculosis (DRT) patients were randomly assigned to a control (Ctrl) group receiving conventional nursing and an experimental (Exp) group treated with continuous nursing intervention (CNI). Quality of Life Instrument for Tuberculosis Patients (QLI-TB) scores were compared over 6 months.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Unmarked NGPs were evenly distributed, while labeled NGPs maintained complete morphology with a gray halo. The detection limit was established at 0.582, reaching as low as 1 pmol\/L. For sputum specimens, detection rates were 38.7% for culture, 41.94% for PCR, and 43.54% for nanogold SSE, with no significant differences (\n                      <jats:italic>P\u2009<\/jats:italic>\n                      &gt;\u20090.05). However, patients in the Exp group exhibited significant improvements in physical, psychological, and social functions, as well as the tuberculosis-specific module (TSM) compared to the Ctrl group (\n                      <jats:italic>P\u2009<\/jats:italic>\n                      &lt;\u20090.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>We demonstrated an innovative method for detecting MTB, demonstrating promising results through method optimization and analysis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/09592989241308792","type":"journal-article","created":{"date-parts":[[2025,1,22]],"date-time":"2025-01-22T04:05:31Z","timestamp":1737518731000},"page":"163-173","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Enhanced detection of Mycobacterium Tuberculosis using nanogold-based silver staining enhancement"],"prefix":"10.1177","volume":"36","author":[{"given":"Lili","family":"Quan","sequence":"first","affiliation":[{"name":"Department of Nursing, Third People's Hospital of Yichang City, Yichang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qianrong","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Tuberculosis, Yichang Third People's Hospital, Yichang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhu","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Tuberculosis, Yichang Third People's Hospital, Yichang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rong","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Tuberculosis, Yichang Third People's Hospital, Yichang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Han","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Tuberculosis, Yichang Third People's Hospital, Yichang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lu","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Tuberculosis, Yichang Third People's Hospital, Yichang, 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