{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T21:03:14Z","timestamp":1777669394303,"version":"3.51.4"},"reference-count":36,"publisher":"SAGE Publications","issue":"12","license":[{"start":{"date-parts":[[2012,5,31]],"date-time":"2012-05-31T00:00:00Z","timestamp":1338422400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Hum Exp Toxicol"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:p>\n                    The effect of the natural compound phenethyl isothiocyanate (PEITC) on cytosolic Ca\n                    <jats:sup>2+<\/jats:sup>\n                    concentrations ([Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>i<\/jats:sub>\n                    ) and viability in MDCK renal cells is unknown. This study explored whether PEITC changed [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>i<\/jats:sub>\n                    in MDCK cells using the Ca\n                    <jats:sup>2+<\/jats:sup>\n                    -sensitive fluorescent dye fura-2. PEITC at 200\u2013700 \u03bcM increased [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>i<\/jats:sub>\n                    in a concentration-dependent manner. The signal was reduced by removing extracellular Ca\n                    <jats:sup>2+<\/jats:sup>\n                    . PEITC-induced Ca\n                    <jats:sup>2+<\/jats:sup>\n                    influx was inhibited by nifedipine, econazole, SK&amp;F 96365 and protein kinase C modulators. In Ca\n                    <jats:sup>2+<\/jats:sup>\n                    -free medium, treatment with the endoplasmic reticulum Ca\n                    <jats:sup>2+<\/jats:sup>\n                    pump inhibitor thapsigargin (TG) or 2,5-di- tert-butylhydroquinone (BHQ) inhibited PEITC-induced rise in [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>i<\/jats:sub>\n                    . Incubation with PEITC also inhibited TG or BHQ-induced rise in [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>i<\/jats:sub>\n                    . Inhibition of phospholipase C with U73122 abolished PEITC-induced rise in [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>i<\/jats:sub>\n                    . At 15\u201375 \u03bcM, PEITC decreased viability. The cytotoxic effect of PEITC was enhanced by chelating cytosolic Ca\n                    <jats:sup>2+<\/jats:sup>\n                    with 1,2-bis(2-aminophenoxy)ethane- N, N, N\u2032, N\u2032-tetraacetic acid\/acetoxymethyl ester. Annexin V-FITC data suggest that 20 and 50 \u03bcM PEITC induced apoptosis. At 10 and 15 \u03bcM, PEITC did not increase reactive oxygen species (ROS) production. Together, in renal tubular cells, PEITC-induced rise in [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>i<\/jats:sub>\n                    by inducing phospholipase C-dependent Ca\n                    <jats:sup>2+<\/jats:sup>\n                    release from endoplasmic reticulum and Ca\n                    <jats:sup>2+<\/jats:sup>\n                    entry via store-operated Ca\n                    <jats:sup>2+<\/jats:sup>\n                    channels. PEITC induced apoptosis in a concentration-dependent, ROS\/Ca\n                    <jats:sup>2+<\/jats:sup>\n                    -independent manner.\n                  <\/jats:p>","DOI":"10.1177\/0960327112446841","type":"journal-article","created":{"date-parts":[[2012,5,31]],"date-time":"2012-05-31T21:27:34Z","timestamp":1338499654000},"page":"1251-1261","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["Effect of phenethyl isothiocyanate on Ca\n                    <sup>2+<\/sup>\n                    movement and viability in MDCK canine renal tubular cells"],"prefix":"10.1177","volume":"31","author":[{"given":"IS","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Surgery, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"KT","family":"Mok","sequence":"additional","affiliation":[{"name":"Department of Surgery, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"CT","family":"Chou","sequence":"additional","affiliation":[{"name":"Division of Basic Medical Sciences, Department of Nursing, Chang Gung Institute of Technology, Chia-Yi, Taiwan"},{"name":"Chronic Diseases and Health Promotion Research Center, Chang Gung Institute of Technology, Chia-Yi, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"SI","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Surgery, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"CC","family":"Kuo","sequence":"additional","affiliation":[{"name":"Department of Nursing, Tzu Hui Institute of Technology, Pingtung, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"SS","family":"Hsu","sequence":"additional","affiliation":[{"name":"Department of Surgery, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"HT","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Surgery, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JY","family":"Tsai","sequence":"additional","affiliation":[{"name":"Department of Surgery, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"WC","family":"Liao","sequence":"additional","affiliation":[{"name":"Department of Surgery, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"CR","family":"Jan","sequence":"additional","affiliation":[{"name":"Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2012,5,31]]},"reference":[{"key":"bibr1-0960327112446841","doi-asserted-by":"publisher","DOI":"10.1080\/01635580802381287"},{"key":"bibr2-0960327112446841","doi-asserted-by":"publisher","DOI":"10.4161\/cbt.6.5.4092"},{"key":"bibr3-0960327112446841","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellbi.2008.09.013"},{"key":"bibr4-0960327112446841","doi-asserted-by":"publisher","DOI":"10.1055\/s-2008-1081307"},{"key":"bibr5-0960327112446841","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2008.06.002"},{"key":"bibr6-0960327112446841","doi-asserted-by":"publisher","DOI":"10.1208\/s12248-008-9032-9"},{"key":"bibr7-0960327112446841","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-07-0340"},{"key":"bibr8-0960327112446841","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)54362-3"},{"key":"bibr9-0960327112446841","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgl087"},{"key":"bibr10-0960327112446841","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2006.03.002"},{"key":"bibr11-0960327112446841","doi-asserted-by":"publisher","DOI":"10.1158\/1535-7163.MCT-05-0041"},{"key":"bibr12-0960327112446841","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-04-1545"},{"key":"bibr13-0960327112446841","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(02)01055-2"},{"key":"bibr14-0960327112446841","doi-asserted-by":"publisher","DOI":"10.1038\/375634a0"},{"key":"bibr15-0960327112446841","doi-asserted-by":"publisher","DOI":"10.1177\/0960327108094606"},{"key":"bibr16-0960327112446841","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-7843.2006.pto_350.x"},{"key":"bibr17-0960327112446841","doi-asserted-by":"publisher","DOI":"10.1080\/10799890500464704"},{"key":"bibr18-0960327112446841","doi-asserted-by":"publisher","DOI":"10.1007\/s002040000130"},{"key":"bibr19-0960327112446841","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)83641-4"},{"key":"bibr20-0960327112446841","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)94218-3"},{"key":"bibr21-0960327112446841","doi-asserted-by":"publisher","DOI":"10.1016\/0278-6915(86)90289-9"},{"key":"bibr22-0960327112446841","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.7.2466"},{"key":"bibr23-0960327112446841","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-05-3809"},{"key":"bibr24-0960327112446841","doi-asserted-by":"publisher","DOI":"10.1002\/cne.21013"},{"key":"bibr25-0960327112446841","doi-asserted-by":"publisher","DOI":"10.1021\/bi00552a018"},{"key":"bibr26-0960327112446841","doi-asserted-by":"publisher","DOI":"10.1016\/0143-4160(86)90026-6"},{"key":"bibr27-0960327112446841","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.170.9.4441"},{"key":"bibr28-0960327112446841","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2003.08.030"},{"key":"bibr29-0960327112446841","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2008.12.031"},{"key":"bibr30-0960327112446841","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceca.2009.02.007"},{"key":"bibr31-0960327112446841","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2008.152157"},{"key":"bibr32-0960327112446841","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2002.022574"},{"key":"bibr33-0960327112446841","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.2001.281.2.C514"},{"key":"bibr34-0960327112446841","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M011241200"},{"key":"bibr35-0960327112446841","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1998.9131"},{"key":"bibr36-0960327112446841","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006935114624"}],"container-title":["Human 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