{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T01:29:26Z","timestamp":1790040566059,"version":"4.0.1"},"reference-count":48,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2012,9,27]],"date-time":"2012-09-27T00:00:00Z","timestamp":1348704000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Hum Exp Toxicol"],"published-print":{"date-parts":[[2013,5]]},"abstract":"<jats:p>\n                    This article describes antioxidative and cytoprotective property of andrographolide, a major active component of the plant Andrographis paniculata ( A. paniculata). High yields (2.7%) of andrographolide was isolated from the aerial parts of this plant via silica column chromatography. The purity of the compound was determined by high-performance thin-layer chromatography (HPTLC) and reversed phase high-performance liquid chromatography (HPLC) analysis. The structure was elucidated using techniques such as UV-visible spectrophotometry, elemental analysis, Fourier transform infrared (FT-IR),\n                    <jats:sup>1<\/jats:sup>\n                    H nuclear magnetic resonance (\n                    <jats:sup>1<\/jats:sup>\n                    H NMR),\n                    <jats:sup>13<\/jats:sup>\n                    C nuclear magnetic resonance (\n                    <jats:sup>13<\/jats:sup>\n                    C NMR) and mass spectral analysis and the data obtained were comparable with reported results. It was observed that andrographolide exhibited significant antioxidative property (IC\n                    <jats:sub>50<\/jats:sub>\n                    = 3.2 \u00b5g\/ml) by its ability to scavenge a stable free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) as compared to known antioxidants like ascorbic acid, butylated hydroxy toluene (BHT) and the plant extract. The cytoprotective role of andrographolide against carbon tetrachloride (CCl\n                    <jats:sub>4<\/jats:sub>\n                    ) toxicity in human hepatoma HepG2 cell line was assessed using trypan blue exclusion test, 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay, by estimation of various leakage enzymes and by measuring the glutathione levels. The recovery obtained for andrographolide treatment in the presence of CCl\n                    <jats:sub>4<\/jats:sub>\n                    was two-fold compared to A. paniculata extract for all other related biochemical parameters investigated. The results of the study indicate that andrographolide is a potent inhibitor of CCl\n                    <jats:sub>4<\/jats:sub>\n                    -mediated lipid peroxidation.\n                  <\/jats:p>","DOI":"10.1177\/0960327112459530","type":"journal-article","created":{"date-parts":[[2012,9,30]],"date-time":"2012-09-30T05:38:30Z","timestamp":1348983510000},"page":"530-543","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":31,"title":["Antioxidative and cytoprotective effects of andrographolide against CCl\n                    <sub>4<\/sub>\n                    -induced hepatotoxicity in HepG2 cells"],"prefix":"10.1177","volume":"32","author":[{"given":"R","family":"Krithika","sequence":"first","affiliation":[{"name":"Zoology Department, School of Sciences, Gujarat University, Ahmedabad, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"RJ","family":"Verma","sequence":"additional","affiliation":[{"name":"Zoology Department, School of Sciences, Gujarat University, Ahmedabad, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"PS","family":"Shrivastav","sequence":"additional","affiliation":[{"name":"Chemistry Department, School of Sciences, Gujarat University, Ahmedabad, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2012,9,27]]},"reference":[{"key":"bibr1-0960327112459530","doi-asserted-by":"publisher","DOI":"10.1016\/0278-6915(86)90298-X"},{"key":"bibr2-0960327112459530","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2621.1996.tb13146.x"},{"key":"bibr3-0960327112459530","doi-asserted-by":"publisher","DOI":"10.1016\/0278-6915(86)90283-8"},{"key":"bibr4-0960327112459530","first-page":"238","volume":"29","author":"Maillard MN","year":"1996","journal-title":"Food 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