{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T03:13:38Z","timestamp":1783480418266,"version":"3.55.0"},"reference-count":41,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2014,6,30]],"date-time":"2014-06-30T00:00:00Z","timestamp":1404086400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Hum Exp Toxicol"],"published-print":{"date-parts":[[2015,3]]},"abstract":"<jats:p>\n                    Zinc (Zn) has long been touted as a panacea for common cold. Recently, there has been some controversy over whether an intranasal (IN) zinc gluconate gel, purported to fight colds, causes anosmia, or loss of the sense of smell, in humans. Previous evidence has shown that IN zinc sulfate (ZnSO\n                    <jats:sub>4<\/jats:sub>\n                    ) solutions can cause anosmia in humans as well as significant damage to the olfactory epithelium in rodents. Using an in vitro olfactory neuron model (the rat Odora cell line), we tested the hypothesis that Zn toxicity was caused by inhibition of the hydrogen voltage-gated channel 1(HVCN1), leading to acidosis and apoptotic cell death. Following studies to characterize the toxicity of zinc gluconate and ZnSO\n                    <jats:sub>4<\/jats:sub>\n                    , Odora cells were grown on coverslips and loaded with 2\u2032,7\u2032-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein acetoxymethyl ester to measure intracellular pH in the presence and absence of Zn salts. While we found that HVCN1 is not functional in Odora cells, we found that olfactory neurons in vitro maintain their intracellular pH through a sodium\/proton exchanger, specifically the sodium proton antiporter 1. ZnSO\n                    <jats:sub>4<\/jats:sub>\n                    , at nontoxic levels, had no impact on intracellular pH after acute exposure or after 24 h of incubation with the cells. In conclusion, Zn toxicity is not mediated through an acidification of intracellular pH in olfactory neurons in vitro.\n                  <\/jats:p>","DOI":"10.1177\/0960327114538985","type":"journal-article","created":{"date-parts":[[2014,7,1]],"date-time":"2014-07-01T12:34:09Z","timestamp":1404218049000},"page":"308-314","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":6,"title":["Evaluation of the toxicity of zinc in the rat olfactory neuronal cell line, Odora"],"prefix":"10.1177","volume":"34","author":[{"given":"H","family":"Hsieh","sequence":"first","affiliation":[{"name":"Department of Environmental Health, University of Cincinnati College of Medicine, Cincinnati, OH, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H","family":"Amlal","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, University of Cincinnati College of Medicine, Cincinnati, OH, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MB","family":"Genter","sequence":"additional","affiliation":[{"name":"Department of Environmental Health, University of Cincinnati College of Medicine, Cincinnati, OH, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2014,6,30]]},"reference":[{"key":"bibr1-0960327114538985","doi-asserted-by":"publisher","DOI":"10.1177\/194589240401800302"},{"key":"bibr2-0960327114538985","first-page":"CD001364","volume":"6","author":"Singh M","year":"2013","journal-title":"Cochrane Database Syst Rev"},{"key":"bibr3-0960327114538985","unstructured":"United States Food and Drug Administration. 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