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Copy number variants (CNVs) play a role in a number of genetic disorders and may be implicated in BDE-209\/BPA teratogenicity. In this study, we found that BDE-209 and\/or BPA exposure decreased neural differentiation efficiency of human embryonic stem cells (hESCs), although there was a &gt;90% induction of neuronal progenitor cells (NPCs) from exposed hESCs. However, the mean of CNV numbers in the NPCs with BDE-209 + BPA treatment was significantly higher compared to the other groups, whereas DNA methylation was lower and DNA methyltransferase(DNMT1 and DNMT3A) expression were significantly decreased in all of the BDE-209 and\/or BPA treatment groups compared with the control groups. The number of CNVs in chromosomes 3, 4, 11, 22, and X in NPCs with BDE-209 and\/or BPA exposure was higher compared to the control group. In addition, CNVs in chromosomes 7, 8, 14, and 16 were stable in hESCs and hESCs-derived NPCs irrespective of BDE-209\/BPA exposure, and CNVs in chromosomes 20 q11.21 and 16 p13.11 might be induced by neural differentiation. Thus, BDE-209\/BPA exposure emerges as a potential source of CNVs distinct from neural differentiation by itself. BDE-209 and\/or BPA exposure may cause genomic instability in cultured stem cells via reduced activity of DNA methyltransferase, suggesting a new mechanism of human embryonic neurodevelopmental toxicity caused by this class of environmental toxins.<\/jats:p>","DOI":"10.1177\/0960327117710535","type":"journal-article","created":{"date-parts":[[2017,6,9]],"date-time":"2017-06-09T05:17:28Z","timestamp":1496985448000},"page":"475-485","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":22,"title":["DNA methylation and copy number variation analyses of human embryonic stem cell\u2013derived neuroprogenitors after low-dose decabromodiphenyl ether and\/or bisphenol A exposure"],"prefix":"10.1177","volume":"37","author":[{"given":"L","family":"Du","sequence":"first","affiliation":[{"name":"The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, People\u2019s Republic of China"},{"name":"Key Laboratory for 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