{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:35:20Z","timestamp":1792787720232,"version":"4.5.0"},"reference-count":23,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2019,12,4]],"date-time":"2019-12-04T00:00:00Z","timestamp":1575417600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"National Science Foundation for Young Scientists of China","award":["81701587"],"award-info":[{"award-number":["81701587"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Hum Exp Toxicol"],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:p>In order to study the effects of chitinase-like protein YKL-40 on proliferation, apoptosis, and migration of human bronchial epithelial cell line (BEAS-2B), and the underlying mechanisms, we cultured BEAS-2B alone or with different concentrations of YKL-40. thiazolyl blue tetrazolium bromide (MTT) assay was used to examine the cell proliferation. Annexin V-fluorescein isothiocyanate isomer (FITC)\/propidium iodide staining and scratch assay were performed to test the cell apoptosis and migration. The concentrations of transforming growth factor-\u03b21 (TGF-\u03b21), Smad3, Smad7, alpha-smooth muscle actin (\u03b1-SMA), interleukin-4 (IL-4), IL-6, and IL-8 in the cell culture supernatant were detected by enzyme-linked immunosorbent assay. The messenger RNA and protein levels of YKL-40, TGF-\u03b21, Smad3, Smad7, and \u03b1-SMA were detected by reverse transcription polymerase chain reaction and Western blot. BEAS-2B cells cultured with different concentrations of YKL-40 showed significantly higher cell proliferation and migration and inflammatory cytokines compared with that of control group, while the cell apoptosis was significantly lower than that of control group ( p &lt; 0.05). In addition, BEAS-2B cells cultured with YKL-40 had increased TGF-\u03b21, Smad3, Smad7, and \u03b1-SMA levels in the supernatant, compared with that of BEAS-2B cells cultured alone ( p &lt; 0.05). Furthermore, LY364947, as TGF-\u03b21\/Smads signaling pathway inhibitor, decreased cell proliferation and migration ability and enhanced cell apoptosis of BEAS-2B cells compared with control group ( p &lt; 0.05). However, YKL-40 administration reversed the effect of LY364947 on the biological behavior of BEAS-2B cells. YKL-40 could affect the biological behaviors of BEAS-2B cells, which might be related to the TGF-\u03b21\/Smads pathway.<\/jats:p>","DOI":"10.1177\/0960327119891218","type":"journal-article","created":{"date-parts":[[2019,12,4]],"date-time":"2019-12-04T05:59:22Z","timestamp":1575439162000},"page":"451-463","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":19,"title":["Chitinase-like protein YKL-40 regulates human bronchial epithelial cells proliferation, apoptosis, and migration through TGF-\u03b21\/Smads pathway"],"prefix":"10.1177","volume":"39","author":[{"given":"R","family":"Guan","sequence":"first","affiliation":[{"name":"Department of Pediatrics, The Affiliated Hospital of Qingdao University, Qingdao, China"},{"name":"Both the authors contributed equally to this work."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, The Affiliated Hospital of Qingdao University, Qingdao, China"},{"name":"Both the authors contributed equally to this work."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, The Affiliated Hospital of Qingdao University, Qingdao, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, The Affiliated Hospital of Qingdao University, Qingdao, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"X","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, The Affiliated Hospital of Qingdao University, Qingdao, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, The Affiliated Hospital of Qingdao University, Qingdao, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6346-7300","authenticated-orcid":false,"given":"L","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, The Affiliated Hospital of Qingdao University, Qingdao, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2019,12,4]]},"reference":[{"key":"bibr1-0960327119891218","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201401050"},{"key":"bibr2-0960327119891218","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201610-2074PP"},{"key":"bibr3-0960327119891218","first-page":"6935","volume":"17","author":"Liu C","year":"2018","journal-title":"Mol Med Rep"},{"key":"bibr4-0960327119891218","doi-asserted-by":"publisher","DOI":"10.1016\/j.rmed.2008.01.017"},{"key":"bibr5-0960327119891218","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/943982"},{"key":"bibr6-0960327119891218","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa073600"},{"key":"bibr7-0960327119891218","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.00800-2017"},{"key":"bibr8-0960327119891218","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201504-0760OC"},{"key":"bibr9-0960327119891218","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/8319283"},{"key":"bibr10-0960327119891218","doi-asserted-by":"publisher","DOI":"10.1186\/s12906-018-2410-7"},{"key":"bibr11-0960327119891218","doi-asserted-by":"publisher","DOI":"10.1016\/S1499-3872(12)60210-0"},{"key":"bibr12-0960327119891218","doi-asserted-by":"publisher","DOI":"10.1177\/039463201302600316"},{"key":"bibr13-0960327119891218","first-page":"1372","volume":"38","author":"Liu H","year":"2018","journal-title":"Nan Fang Yi De Da Xue Xue Bao"},{"key":"bibr14-0960327119891218","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1201827"},{"key":"bibr15-0960327119891218","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1111\/j.1365-3083.2010.02404.x","volume":"72","author":"Hong JY","year":"2010","journal-title":"Scand J Immunol"},{"key":"bibr16-0960327119891218","doi-asserted-by":"publisher","DOI":"10.1080\/01902148.2018.1522558"},{"key":"bibr17-0960327119891218","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0203211"},{"key":"bibr18-0960327119891218","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.26310"},{"key":"bibr19-0960327119891218","doi-asserted-by":"publisher","DOI":"10.1016\/j.alit.2017.02.010"},{"key":"bibr20-0960327119891218","doi-asserted-by":"publisher","DOI":"10.3109\/02770903.2011.611955"},{"key":"bibr21-0960327119891218","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20081271"},{"key":"bibr22-0960327119891218","doi-asserted-by":"publisher","DOI":"10.1186\/s12890-019-0906-7"},{"key":"bibr23-0960327119891218","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/215140"}],"container-title":["Human &amp; 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