{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T02:12:24Z","timestamp":1791425544990,"version":"4.3.3"},"reference-count":34,"publisher":"SAGE Publications","issue":"7","license":[{"start":{"date-parts":[[2021,1,5]],"date-time":"2021-01-05T00:00:00Z","timestamp":1609804800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Hum Exp Toxicol"],"published-print":{"date-parts":[[2021,7]]},"abstract":"<jats:p>Psoriasis is a common immune-mediated and genetic skin disease. Forkhead box M1 (FOXM1) is a member of FOX family that has been found to modulate skin disorders. However, its role in psoriasis remains unknown. Thus, we aimed to investigate the effect of FOXM1 on keratinocytes in response to tumor necrosis factor-\u03b1 (TNF-\u03b1). The expression levels of FOXM1 in psoriasis tissues and normal skin tissues were examined using qRT-PCR and western blot. HaCaT cells were stimulated by TNF-\u03b1 to mimic psoriasis in vitro. MTT assay was performed to assess cell proliferation. The caspase-3 activity and expression levels of bcl-2 and bax were determined to indicate cell apoptosis. The mRNA and secretion levels of IL-6, IL-23 and TGF-\u03b2 were determined by qRT-PCR and ELISA, respectively. The NF-\u03baB activation was assessed using western blot analysis. Our results demonstrated that FOXM1 was highly upregulated in psoriatic skin tissues and TNF-\u03b1-stimulated HaCaT cells. Knockdown of FOXM1 repressed cell proliferation of TNF-\u03b1-stimulated HaCaT cells. Knockdown of FOXM1 caused significant increases in caspase-3 activity, bax expression and decrease in bcl-2 expression in TNF-\u03b1-stimulated HaCaT cells. Moreover, FOXM1 knockdown also suppressed the TNF-\u03b1-induced production of IL-6, IL-23, and TGF-\u03b2 in HaCaT cells. However, FOXM1 overexpression showed the opposite effect. Furthermore, the TNF-\u03b1-induced NF-\u03baB activation was prevented by FOXM1 knockdown. Additionally, inhibition of NF-\u03baB reversed the effects of FOXM1 on HaCaT cells. Taken together, these findings indicated that FOXM1 regulated cell proliferation, apoptosis and inflammation in TNF-\u03b1-induced HaCaT cells. The effects of FOXM1 were mediated by NF-\u03baB pathway.<\/jats:p>","DOI":"10.1177\/0960327120984225","type":"journal-article","created":{"date-parts":[[2021,1,13]],"date-time":"2021-01-13T18:08:06Z","timestamp":1610561286000},"page":"1130-1140","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":21,"title":["FOXM1 regulates the proliferation, apoptosis and inflammatory response of keratinocytes through the NF-\u03baB signaling pathway"],"prefix":"10.1177","volume":"40","author":[{"given":"Mi","family":"Zhou","sequence":"first","affiliation":[{"name":"Department of Dermatology, Affiliated Hospital of Shaoxing University (Shaoxing Municipal Hospital), Shaoxing, China"},{"name":"Both the authors contributed equally to this paper."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Tianjin Academy of Traditional Chinese Medicine Affiliated Hospital, Tianjin, China"},{"name":"Both the authors contributed equally to this paper."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaobo","family":"Lan","sequence":"additional","affiliation":[{"name":"Department of Hepatology, Affiliated Hospital of Shaoxing University (Shaoxing Municipal Hospital), Shaoxing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9463-7616","authenticated-orcid":false,"given":"Xianjun","family":"Gong","sequence":"additional","affiliation":[{"name":"Department of Dermatology, ZaoZhuang Municipal Hospital, Zaozhuang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2021,1,5]]},"reference":[{"key":"bibr1-0960327120984225","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2230.2001.00832.x"},{"key":"bibr2-0960327120984225","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(14)61909-7"},{"key":"bibr3-0960327120984225","doi-asserted-by":"publisher","DOI":"10.1007\/s40272-015-0137-1"},{"key":"bibr4-0960327120984225","first-page":"S14","volume":"33","author":"Oji V","year":"2015","journal-title":"Clin Exp Rheumatol"},{"key":"bibr5-0960327120984225","doi-asserted-by":"publisher","DOI":"10.1016\/j.diff.2020.08.002"},{"key":"bibr6-0960327120984225","first-page":"1495","volume":"25","author":"Katoh M","year":"2004","journal-title":"Int J Oncol"},{"key":"bibr7-0960327120984225","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-16-3566"},{"key":"bibr8-0960327120984225","doi-asserted-by":"publisher","DOI":"10.1186\/s12964-019-0347-1"},{"key":"bibr9-0960327120984225","doi-asserted-by":"publisher","DOI":"10.1007\/s00109-017-1619-0"},{"key":"bibr10-0960327120984225","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.27583"},{"key":"bibr11-0960327120984225","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2017.11.106"},{"key":"bibr12-0960327120984225","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2018.12.057"},{"key":"bibr13-0960327120984225","doi-asserted-by":"publisher","DOI":"10.18632\/aging.100988"},{"key":"bibr14-0960327120984225","doi-asserted-by":"publisher","DOI":"10.1186\/1476-4598-9-45"},{"key":"bibr15-0960327120984225","doi-asserted-by":"publisher","DOI":"10.1016\/j.rdc.2015.07.013"},{"key":"bibr16-0960327120984225","doi-asserted-by":"publisher","DOI":"10.1016\/j.det.2014.09.002"},{"key":"bibr17-0960327120984225","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.01549"},{"key":"bibr18-0960327120984225","doi-asserted-by":"publisher","DOI":"10.1159\/000495291"},{"key":"bibr19-0960327120984225","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0625.2007.00629.x"},{"key":"bibr20-0960327120984225","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-032713-120225"},{"key":"bibr21-0960327120984225","doi-asserted-by":"publisher","DOI":"10.1016\/1043-4666(90)90045-U"},{"key":"bibr22-0960327120984225","doi-asserted-by":"publisher","DOI":"10.1007\/s12026-020-09149-1"},{"key":"bibr23-0960327120984225","doi-asserted-by":"publisher","DOI":"10.1038\/ni1497"},{"key":"bibr24-0960327120984225","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201040391"},{"key":"bibr25-0960327120984225","doi-asserted-by":"publisher","DOI":"10.1111\/imm.12938"},{"key":"bibr26-0960327120984225","doi-asserted-by":"publisher","DOI":"10.2741\/e777"},{"key":"bibr27-0960327120984225","doi-asserted-by":"publisher","DOI":"10.3109\/07853890.2011.577093"},{"key":"bibr28-0960327120984225","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2013.11.008"},{"key":"bibr29-0960327120984225","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2010.05825.x"},{"key":"bibr30-0960327120984225","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.2012.01099.x"},{"key":"bibr31-0960327120984225","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2011.13"},{"key":"bibr32-0960327120984225","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2012.11.002"},{"key":"bibr33-0960327120984225","doi-asserted-by":"publisher","DOI":"10.2174\/187221309787158399"},{"key":"bibr34-0960327120984225","doi-asserted-by":"publisher","DOI":"10.1016\/j.cancergen.2014.02.008"}],"container-title":["Human &amp; Experimental Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0960327120984225","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/0960327120984225","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0960327120984225","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T07:50:16Z","timestamp":1777449016000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/0960327120984225"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,1,5]]},"references-count":34,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2021,7]]}},"alternative-id":["10.1177\/0960327120984225"],"URL":"https:\/\/doi.org\/10.1177\/0960327120984225","relation":{},"ISSN":["0960-3271","1477-0903"],"issn-type":[{"value":"0960-3271","type":"print"},{"value":"1477-0903","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,1,5]]}}}