{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T13:28:02Z","timestamp":1779888482248,"version":"3.53.1"},"reference-count":45,"publisher":"SAGE Publications","issue":"10","license":[{"start":{"date-parts":[[2021,3,29]],"date-time":"2021-03-29T00:00:00Z","timestamp":1616976000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Hum Exp Toxicol"],"published-print":{"date-parts":[[2021,10]]},"abstract":"<jats:sec>\n                    <jats:title>Objective:<\/jats:title>\n                    <jats:p>To explore the effects of myocardin-related transcription factor A (MRTF-A) knockout on mice with nonalcoholic steatohepatitis (NASH) induced by high-fat diet (HFD).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>\n                      Normal-fat diet (NFD) or HFD was fed to MRTF-A-knockout (MRTF-A\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                      ) and wild-type (WT) mice for 16 weeks. Liver histopathological status was observed using Hematoxylin and Eosin (HE) staining, Oil Red O staining, Sirius Red staining, and Immunohistochemical staining. The mRNA and protein levels in liver tissues were measured through quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and Western blot.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      Compared with WT + HFD group, mice in MRTF-A\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                      + HFD group were decreased in body weight, blood glucose, plasma insulin, liver TG and NAFLD activity score (NAS), with liver function recovery. Besides, compared with HFD-fed WT mice, HFD-fed MRTF-A\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                      mice were improved in hepatic fibrosis, accompanied by decreased collagen content (%) and down-regulated expressions of \u03b1-SMA, COL1A2, TGF\u03b21, and SMAD3. In mice fed with HFD, the expression of MCP-1, CCR2, F4\/80 and CD68 declined in liver tissues of MRTF-A\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                      mice as compared with WT mice. Besides, in hepatic macrophages isolated from HFD-fed mice, the observed increased expression of TNF-\u03b1, IL-1\u03b2, MCP-1, as well as decreased expression of CCR2. Compared with WT + HFD group, MRTF-A\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                      + HFD group mice were decreased regarding NF-\u03baB p65 in liver tissues.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>MRTF-A knockout reduced macrophage infiltration, down-regulated NF-\u03baB p65 expression, and ameliorated inflammation and fibrosis of liver tissues in mice, thereby becoming a potential therapeutic target for NASH treatment.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/09603271211002886","type":"journal-article","created":{"date-parts":[[2021,3,29]],"date-time":"2021-03-29T05:42:40Z","timestamp":1616996560000},"page":"1634-1645","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":6,"title":["Therapeutic effects of myocardin-related transcription factor A (MRTF-A) knockout on experimental mice with nonalcoholic steatohepatitis induced by high-fat diet"],"prefix":"10.1177","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3918-9926","authenticated-orcid":false,"given":"Lei","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Infectious Diseases, Yantai Affiliated Hospital of Binzhou Medical University, Yantai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua-Long","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Yantai Affiliated Hospital of Binzhou Medical University, Yantai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ding-Ding","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Yantai Affiliated Hospital of Binzhou Medical University, Yantai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Chun","family":"Cui","sequence":"additional","affiliation":[{"name":"Bone and Joint Surgery, Yantai Affiliated Hospital of Binzhou Medical University, Yantai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2021,3,29]]},"reference":[{"key":"bibr1-09603271211002886","doi-asserted-by":"publisher","DOI":"10.1002\/ca.22350"},{"key":"bibr2-09603271211002886","doi-asserted-by":"publisher","DOI":"10.1016\/j.nut.2020.111092"},{"key":"bibr3-09603271211002886","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22010436"},{"key":"bibr4-09603271211002886","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2020.597648"},{"key":"bibr5-09603271211002886","doi-asserted-by":"publisher","DOI":"10.1111\/1751-2980.12967"},{"key":"bibr6-09603271211002886","doi-asserted-by":"publisher","DOI":"10.1002\/pbc.28111"},{"key":"bibr7-09603271211002886","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00302.2019"},{"key":"bibr8-09603271211002886","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.23.18.6597-6608.2003"},{"key":"bibr9-09603271211002886","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0229888"},{"key":"bibr10-09603271211002886","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2019.05.015"},{"issue":"7","key":"bibr11-09603271211002886","first-page":"1495","volume":"31","author":"Kuwahara K","year":"2012","journal-title":"Arterioscler Thromb Vasc Biol"},{"key":"bibr12-09603271211002886","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.12.005"},{"key":"bibr13-09603271211002886","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2014.07.029"},{"key":"bibr14-09603271211002886","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2018.06.027"},{"key":"bibr15-09603271211002886","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbi.2020.109199"},{"key":"bibr16-09603271211002886","doi-asserted-by":"crossref","first-page":"8962458","DOI":"10.1155\/2017\/8962458","volume":"2017","author":"Blasetti Fantauzzi C","year":"2017","journal-title":"Oxidat Med Cell Long"},{"key":"bibr17-09603271211002886","unstructured":"Institute of Laboratory Animal Resources (US). 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