{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T20:31:19Z","timestamp":1777667479570,"version":"3.51.4"},"reference-count":24,"publisher":"SAGE Publications","issue":"12_suppl","license":[{"start":{"date-parts":[[2021,8,9]],"date-time":"2021-08-09T00:00:00Z","timestamp":1628467200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"Social Development Projects of Shaanxi Province","award":["2019SF-004"],"award-info":[{"award-number":["2019SF-004"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82072722"],"award-info":[{"award-number":["82072722"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Hum Exp Toxicol"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:p>Background:\u00a0Several recent studies have suggested that the long non-coding RNA (lncRNA) DSCAM-AS1 (Down syndrome cell adhesion molecule - anti-sense 1) is aberrantly expressed in many malignancies. Purpose:\u00a0In this study, we aimed to explore the the role of DSCAM-AS1 in gastric carcinoma. Research Design:\u00a0Expression of DSCAM-AS1 mRNA, miR-204, and TPT1 (Tumor Protein, Translationally-Controlled 1) were detected using quantitative real-time polymerase chain reaction (qRT-PCR). Proliferation and apoptosis of GC cells were determined using the CCK-8 cell counting assay and flow cytometry. The rate of cell migration and invasion was determined using a transwell assay. The relationships between DSCAM-AS1, miR-204, and TPT1 were predicted and confirmed using a dual-luciferase reporter assay. Expression of TPT1 protein was quantified by Western blot. Results:\u00a0In this study, we found that DSCAM-AS1 was significantly overexpressed in GC tissues and cell lines. Functional experiments indicated that GC cells with DSCAM-AS1 silencing exhibited a dynamic reduction in proliferation and migration. We identified miR-204 as a target of DSCAM-AS1 and found that it targeted TPT1 in GC cells, which further led to decreased expression of miR-204 in GC tissues and cell lines. A rescue assay revealed that knocked-down DSCAM-AS1 hindered GC progression, which was reversed upon miR-204 downregulation or TPT1 overexpression. Conclusion:\u00a0We conclude that DSCAM-AS1 is expressed as a tumor oncogene in GC progression, modulated via the miR-204\/TPT1 axis. These findings indicate the potential of DSCAM-AS1 as a therapeutic target for GC prevention.<\/jats:p>","DOI":"10.1177\/09603271211036037","type":"journal-article","created":{"date-parts":[[2021,8,9]],"date-time":"2021-08-09T22:55:42Z","timestamp":1628549742000},"page":"S187-S195","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":8,"title":["Long non-coding RNA Down syndrome cell adhesion molecule-anti-sense 1 promotes gastric carcinoma cell proliferation and migration by regulating the miR-204\/TPT1 axis"],"prefix":"10.1177","volume":"40","author":[{"given":"Nan","family":"Wang","sequence":"first","affiliation":[{"name":"Department of General Surgery, Tangdu Hospital, The Air Force Medical University, Xi\u2019an, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Tangdu Hospital, The Air Force Medical University, Xi\u2019an, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guo-Zhan","family":"Jia","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Tangdu Hospital, The Air Force Medical University, Xi\u2019an, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ke","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Tangdu Hospital, The Air Force Medical University, Xi\u2019an, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuai","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Tangdu Hospital, The Air Force Medical University, Xi\u2019an, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bo","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Tangdu Hospital, The Air Force Medical University, Xi\u2019an, 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