{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T05:40:42Z","timestamp":1781502042039,"version":"3.54.1"},"reference-count":37,"publisher":"SAGE Publications","issue":"12_suppl","license":[{"start":{"date-parts":[[2021,11,9]],"date-time":"2021-11-09T00:00:00Z","timestamp":1636416000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"Program for Science and Technology Innovation Talents in Higher Education of Henan Province","award":["20HASTIT046"],"award-info":[{"award-number":["20HASTIT046"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural and Science Foundation of China","doi-asserted-by":"crossref","award":["U1904132"],"award-info":[{"award-number":["U1904132"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Key Scientific Research Project of Universities in Henan","award":["19A180005"],"award-info":[{"award-number":["19A180005"]}]},{"name":"Key Science and Technology Project of Xinxiang","award":["GG2019008"],"award-info":[{"award-number":["GG2019008"]}]},{"name":"Training Program for Excellent Young Teachers in Higher Education of Henan Province","award":["2017GGJS110"],"award-info":[{"award-number":["2017GGJS110"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Hum Exp Toxicol"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:p>\n                    Background: Nicotinamide phosphoribosyltransferase (NAMPT) catalyzes the rate-limiting step in the salvage pathway of mammalian nicotinamide adenine dinucleotide (NAD\n                    <jats:sup>+<\/jats:sup>\n                    ) biosynthesis. Through its NAD\n                    <jats:sup>+<\/jats:sup>\n                    -biosynthetic activity, NAMPT is able to regulate the development of hepatic steatosis and inflammation induced by diet or alcohol. However, the roles NAMPT plays in the development of liver fibrosis remain obscure.\u00a0Purpose: To investigate the roles of NAMPT-mediated NAD\n                    <jats:sup>+<\/jats:sup>\n                    \u00a0biosynthesis in hepatic stellate cell (HSC) activation and liver fibrosis.\u00a0Research Design: Realtime RT-PCR and western blot analyses were performed to analyze the expression of profibrogenic genes. Sirius red staining was conducted to examine the fibrosis in liver. Mouse liver fibrosis was induced by intraperitoneal injection of carbon tetrachloride (CCl\n                    <jats:sub>4<\/jats:sub>\n                    ) 2 times a week for 6 weeks. Adenovirus-mediated NAMPT overexpression or nicotinamide mononucleotide (NMN) administration was carried out to study the effects of elevation of NAD\n                    <jats:sup>+<\/jats:sup>\n                    \u00a0levels on protecting CCl\n                    <jats:sub>4<\/jats:sub>\n                    -induced liver fibrosis in mice. LX2 cells or primary HSCs were used to study the role of NAMPT overexpression or NMN treatment in reducing profibrogenic gene expression in vitro.\u00a0ResultsCCl\n                    <jats:sub>4<\/jats:sub>\n                    \u00a0administration suppresses NAMPT expression in liver and reduces hepatic NAD\n                    <jats:sup>+<\/jats:sup>\n                    \u00a0content. Tgf\u03b21 treatment decreases intracellular NAD\n                    <jats:sup>+<\/jats:sup>\n                    \u00a0levels and NAMPT expression in LX2 cells. Adenovirus-mediated NAMPT overexpression augments liver NAD\n                    <jats:sup>+<\/jats:sup>\n                    \u00a0levels, inhibits HSC activation and alleviates CCl\n                    <jats:sub>4<\/jats:sub>\n                    -induced liver fibrosis in mice. Administration of NMN also suppresses HSC activation and protects against CCl\n                    <jats:sub>4<\/jats:sub>\n                    -induced liver fibrosis in mice.\u00a0Conclusions: NAMPT-mediated NAD\n                    <jats:sup>+<\/jats:sup>\n                    \u00a0biosynthesis inhibits HSC activation and protects against CCl\n                    <jats:sub>4<\/jats:sub>\n                    -induced liver fibrosis.\n                  <\/jats:p>","DOI":"10.1177\/09603271211052991","type":"journal-article","created":{"date-parts":[[2021,11,9]],"date-time":"2021-11-09T13:00:05Z","timestamp":1636462805000},"page":"S666-S675","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":7,"title":["NAMPT-mediated NAD\n                    <sup>+<\/sup>\n                    biosynthesis suppresses activation of hepatic stellate cells and protects against CCl\n                    <sub>4<\/sub>\n                    -induced liver fibrosis in mice"],"prefix":"10.1177","volume":"40","author":[{"given":"Lin","family":"Xu","sequence":"first","affiliation":[{"name":"School of Forensic Medicine, Xinxiang Medical University, Xinxiang, China"},{"name":"Department of Medical Genetics, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenyan","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Forensic Medicine, Xinxiang Medical University, Xinxiang, China"},{"name":"Xinxiang Key Laboratory of Metabolism and Integrative Physiology, Xinxiang Medical University, Xinxiang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Ma","sequence":"additional","affiliation":[{"name":"Xinxiang Key Laboratory of Metabolism and Integrative Physiology, Xinxiang Medical University, Xinxiang, China"},{"name":"Department of Human Anatomy and Histoembryology, School of Basic Medical Sciences, Xinxiang Medical University, Henan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinge","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Forensic Medicine, Xinxiang Medical University, Xinxiang, China"},{"name":"Xinxiang Key Laboratory of Metabolism and Integrative Physiology, Xinxiang Medical University, Xinxiang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0666-230X","authenticated-orcid":false,"given":"Qingzhi","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Forensic Medicine, Xinxiang Medical University, Xinxiang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5802-4994","authenticated-orcid":false,"given":"Xiwen","family":"Xiong","sequence":"additional","affiliation":[{"name":"School of Forensic Medicine, Xinxiang Medical University, Xinxiang, China"},{"name":"Xinxiang Key Laboratory of Metabolism and Integrative Physiology, Xinxiang Medical University, Xinxiang, 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