{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T20:32:07Z","timestamp":1777667527677,"version":"3.51.4"},"reference-count":30,"publisher":"SAGE Publications","issue":"12_suppl","license":[{"start":{"date-parts":[[2021,11,17]],"date-time":"2021-11-17T00:00:00Z","timestamp":1637107200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100004410","name":"TUBITAK","doi-asserted-by":"crossref","award":["SBAG-113S252"],"award-info":[{"award-number":["SBAG-113S252"]}],"id":[{"id":"10.13039\/501100004410","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Hum Exp Toxicol"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:p>\n                    The species belonging to Scrophularia genus grow mainly in Irano-Turanian and Mediterranean regions and have been used as folk remedy for inflammatory-related diseases since ancient times. The present study was aimed to evaluate the anti-inflammatory activity of the extracts of Scrophularia kotschyana as well as the isolated compounds. The aerial parts and the roots of the plant were separately extracted with methanol. Anti-inflammatory activities of both extracts were evaluated with formalin test in mice. As the methanolic extract of the aerial parts significantly ( p &lt; .05) inhibited inflammation, it was then submitted to successive solvent extractions with n-hexane, dichloromethane, ethyl acetate and n-butanol to yield subextracts. Anti-inflammatory activities of the subextracts were evaluated within the same test system. Among the subextracts tested, the n-butanol subextract produced a significant ( p &lt; .05) anti-inflammatory activity at all doses (5, 10, and 30\u00a0mg\/kg, ip.). Sequential chromatographic separation of the n-butanol subextract yielded 8-O-acetyl-4\n                    <jats:sup>\u2032<\/jats:sup>\n                    - O-( E)- p-coumaroylharpagide, 8- O-acetyl-4\n                    <jats:sup>\u2032<\/jats:sup>\n                    - O-( Z)- p-coumaroylharpagide, \u03b2-sitosterol 3- O-\u03b2-glucopyranoside, apigenin 7- O-\u03b2-glucopyranoside, apigenin 7- O-rutinoside, luteolin 7- O-\u03b2-glucopyranoside and luteolin 7- O-rutinoside. The anti-inflammatory activities of the isolates were evaluated at 5\u00a0mg\/kg dose. Luteolin 7- O-\u03b2-glucopyranoside and apigenin 7- O-rutinoside caused a significant ( p &lt; .05) inhibition of oedema formation.\n                  <\/jats:p>","DOI":"10.1177\/09603271211058889","type":"journal-article","created":{"date-parts":[[2021,11,17]],"date-time":"2021-11-17T05:28:32Z","timestamp":1637126912000},"page":"S676-S683","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":8,"title":["Anti-inflammatory secondary metabolites from\n                    <i>Scrophularia kotschyana<\/i>"],"prefix":"10.1177","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6323-0338","authenticated-orcid":false,"given":"G\u00fclin","family":"Renda","sequence":"first","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy, Karadeniz Technical University, Trabzon, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mine","family":"Kad\u0131o\u011flu","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Merve","family":"K\u0131l\u0131\u00e7","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B\u00fc\u015fra","family":"Korkmaz","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy, Karadeniz Technical University, Trabzon, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6118-8225","authenticated-orcid":false,"given":"Hasan","family":"K\u0131rm\u0131z\u0131bekmez","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy, Yeditepe University,Istanbul, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2021,11,17]]},"reference":[{"key":"bibr1-09603271211058889","first-page":"603","volume-title":"Flora of Turkey and the East Aegean Islands","volume":"6","author":"Lall SS","year":"1978"},{"key":"bibr2-09603271211058889","first-page":"97","volume-title":"Flora Iberica","author":"Olivencia AO","year":"2009"},{"key":"bibr3-09603271211058889","doi-asserted-by":"publisher","DOI":"10.11646\/phytotaxa.204.1.10"},{"key":"bibr4-09603271211058889","doi-asserted-by":"publisher","DOI":"10.1076\/phbi.40.1.45.5864"},{"key":"bibr5-09603271211058889","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2005.03.023"},{"key":"bibr6-09603271211058889","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2015.10.022"},{"key":"bibr7-09603271211058889","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2015.06.052"},{"key":"bibr8-09603271211058889","doi-asserted-by":"publisher","DOI":"10.1016\/j.sbspro.2011.05.195"},{"key":"bibr9-09603271211058889","doi-asserted-by":"publisher","DOI":"10.1663\/0013-0001(2004)058[0697:EAOSTI]2.0.CO;2"},{"key":"bibr10-09603271211058889","doi-asserted-by":"publisher","DOI":"10.1080\/14786419.2017.1356829"},{"key":"bibr11-09603271211058889","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2010.05.060"},{"key":"bibr12-09603271211058889","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3959(00)00348-1"},{"key":"bibr13-09603271211058889","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-016-9737-1"},{"key":"bibr14-09603271211058889","doi-asserted-by":"publisher","DOI":"10.1007\/BF01234480"},{"key":"bibr15-09603271211058889","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4612-0813-6_3"},{"key":"bibr16-09603271211058889","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3959(92)90003-T"},{"key":"bibr17-09603271211058889","doi-asserted-by":"publisher","DOI":"10.1078\/094471103321659924"},{"key":"bibr18-09603271211058889","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3959(87)90088-1"},{"key":"bibr19-09603271211058889","doi-asserted-by":"publisher","DOI":"10.1080\/13880209.2017.1397178"},{"key":"bibr20-09603271211058889","doi-asserted-by":"publisher","DOI":"10.17795\/jjnpp-7621"},{"key":"bibr21-09603271211058889","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.26.462"},{"key":"bibr22-09603271211058889","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2013.03.048"},{"key":"bibr23-09603271211058889","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2011.06.069"},{"key":"bibr24-09603271211058889","doi-asserted-by":"publisher","DOI":"10.1016\/S2222-1808(15)61019-2"},{"key":"bibr25-09603271211058889","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnutbio.2014.11.008"},{"key":"bibr26-09603271211058889","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainresbull.2015.09.002"},{"key":"bibr27-09603271211058889","doi-asserted-by":"publisher","DOI":"10.1002\/bdd.1956"},{"issue":"47","key":"bibr28-09603271211058889","first-page":"e47\u201315","volume":"5","author":"Panche AN","year":"2016","journal-title":"J Nutr Sci"},{"key":"bibr29-09603271211058889","first-page":"7010467","author":"Wang M","year":"2019","journal-title":"BioMed Research International"},{"key":"bibr30-09603271211058889","doi-asserted-by":"publisher","DOI":"10.1007\/s00044-016-1681-0"}],"container-title":["Human &amp; Experimental Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/09603271211058889","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/09603271211058889","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/09603271211058889","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T07:47:02Z","timestamp":1777448822000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/09603271211058889"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11,17]]},"references-count":30,"journal-issue":{"issue":"12_suppl","published-print":{"date-parts":[[2021,12]]}},"alternative-id":["10.1177\/09603271211058889"],"URL":"https:\/\/doi.org\/10.1177\/09603271211058889","relation":{},"ISSN":["0960-3271","1477-0903"],"issn-type":[{"value":"0960-3271","type":"print"},{"value":"1477-0903","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,11,17]]}}}