{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T20:24:33Z","timestamp":1777667073224,"version":"3.51.4"},"reference-count":53,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Hum Exp Toxicol"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Long noncoding RNAs (lncRNAs) are involved in preeclampsia (PE), and apolipoprotein A-1 antisense RNA (APOA1-AS) and has been found to be associated with a number of diseases. Our study aims to understand the involvement of APOA1-AS in PE.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Clinically, APOA1-AS expression in early-onset severe PE (EOSPE) patients and healthy controls was detected by real-time quantitative polymerase chain reaction. In vitro experiments were divided into control [coculturing trophoblasts with human uterine microvascular endothelial cells (UtMVECs)], TNF-\u03b1 [coculturing trophoblasts with UtMVECs treated with tumor necrosis factor-\u03b1 (TNF-\u03b1)], and TNF-\u03b1 + control siRNA\/APOA1-AS siRNA groups (UtMVECs transfected with control siRNA\/APOA1-AS siRNA were cocultured with trophoblasts in the presence of TNF-\u03b1). The animals were divided into normal group, PE group (PE model was established by administrating nitro-L-arginine methyl ester (L-NAME) in rats), PE + control siRNA group (PE rats were treated with control siRNA), and PE + APOA1-AS siRNA group (PE rats were treated with APOA1-AS siRNA).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Increased APOA1-AS was found in the placental tissues of EOSPE patients. APOA1-AS siRNA abolished the decreased integration of trophoblasts into UtMVEC networks induced by TNF-\u03b1. Furthermore, APOA1-AS siRNA improved pregnancy outcomes in PE rats with increased expression of vascular endothelial growth factor, placental growth factor, and fms-like tyrosine kinase receptor (Flt-1) but decreased expression of E-cadherin, intercellular cell adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Downregulation of APOA1-AS protected against TNF-\u03b1-induced inhibition of trophoblast integration into endothelial networks, thus exerting protective effects against PE rats.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/09603271211066586","type":"journal-article","created":{"date-parts":[[2022,2,7]],"date-time":"2022-02-07T23:54:45Z","timestamp":1644278085000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":6,"title":["The involvement of long noncoding RNA APOA1-AS in the pathogenesis of preeclampsia"],"prefix":"10.1177","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3056-9754","authenticated-orcid":false,"given":"Hai-Yan","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Obstetrics, Yantaishan Hospital, Yantai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3125-1518","authenticated-orcid":false,"given":"Ling","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Yantaishan Hospital, Yantai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2022,2,7]]},"reference":[{"key":"bibr1-09603271211066586","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.118.313276"},{"key":"bibr2-09603271211066586","doi-asserted-by":"publisher","DOI":"10.1055\/s-0037-1604471"},{"key":"bibr3-09603271211066586","doi-asserted-by":"publisher","DOI":"10.3390\/ijms18071371"},{"key":"bibr4-09603271211066586","doi-asserted-by":"publisher","DOI":"10.1080\/10641955.2020.1855192"},{"key":"bibr5-09603271211066586","doi-asserted-by":"publisher","DOI":"10.1002\/iub.2199"},{"key":"bibr6-09603271211066586","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2021.1883881"},{"key":"bibr7-09603271211066586","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2020.559478"},{"key":"bibr8-09603271211066586","doi-asserted-by":"publisher","DOI":"10.1016\/j.omtn.2020.09.033"},{"key":"bibr9-09603271211066586","doi-asserted-by":"publisher","DOI":"10.1017\/S0967199420000507"},{"key":"bibr10-09603271211066586","doi-asserted-by":"publisher","DOI":"10.1590\/1414-431x20209732"},{"key":"bibr11-09603271211066586","doi-asserted-by":"publisher","DOI":"10.1177\/0963689720965182"},{"key":"bibr12-09603271211066586","doi-asserted-by":"publisher","DOI":"10.1080\/10715762.2020.1826468"},{"key":"bibr13-09603271211066586","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2020.127640"},{"key":"bibr14-09603271211066586","doi-asserted-by":"publisher","DOI":"10.1016\/j.jare.2019.10.012"},{"key":"bibr15-09603271211066586","doi-asserted-by":"publisher","DOI":"10.1007\/s10067-018-4269-x"},{"key":"bibr16-09603271211066586","doi-asserted-by":"publisher","DOI":"10.1016\/j.omtn.2021.03.010"},{"key":"bibr17-09603271211066586","doi-asserted-by":"crossref","first-page":"1122","DOI":"10.1097\/01.AOG.0000437382.03963.88","volume":"122","author":"Author anonymous","year":"2013","journal-title":"Obstet Gynecol"},{"key":"bibr18-09603271211066586","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00331.2016"},{"key":"bibr19-09603271211066586","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-27296-2"},{"key":"bibr20-09603271211066586","first-page":"3125","volume":"42","author":"Feng J","year":"2018","journal-title":"Int J Mol Med"},{"key":"bibr21-09603271211066586","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.11110"},{"key":"bibr22-09603271211066586","unstructured":"Ukwuani-Kwaja AN. Antihypertensive activity of Hibiscus Sabdariffa aqueous calyx extract in Albino rats, 2015."},{"key":"bibr23-09603271211066586","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2013.12.015"},{"key":"bibr24-09603271211066586","first-page":"1653","volume":"11","author":"Wali RK","year":"2002","journal-title":"Cancer Epidemiol. Biomark. Prev"},{"key":"bibr25-09603271211066586","doi-asserted-by":"publisher","DOI":"10.1007\/s00383-003-1012-7"},{"key":"bibr26-09603271211066586","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.52.5.1222"},{"key":"bibr27-09603271211066586","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkr1013"},{"key":"bibr28-09603271211066586","doi-asserted-by":"publisher","DOI":"10.1016\/j.livres.2017.09.001"},{"key":"bibr29-09603271211066586","doi-asserted-by":"publisher","DOI":"10.1177\/147323001103900430"},{"key":"bibr30-09603271211066586","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21103597"},{"key":"bibr31-09603271211066586","doi-asserted-by":"publisher","DOI":"10.1186\/s13104-019-4865-0"},{"key":"bibr32-09603271211066586","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2010.12.005"},{"key":"bibr33-09603271211066586","doi-asserted-by":"publisher","DOI":"10.1016\/j.preghy.2012.04.042"},{"key":"bibr34-09603271211066586","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2014.04.010"},{"key":"bibr35-09603271211066586","doi-asserted-by":"publisher","DOI":"10.3346\/jkms.2004.19.5.688"},{"key":"bibr36-09603271211066586","doi-asserted-by":"publisher","DOI":"10.1007\/s12020-012-9644-9"},{"key":"bibr37-09603271211066586","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2016.04.015"},{"key":"bibr38-09603271211066586","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(89)90775-7"},{"key":"bibr39-09603271211066586","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.15.11.1987"},{"key":"bibr40-09603271211066586","first-page":"825","volume":"45","author":"Lin ZH","year":"2020","journal-title":"Int J Mol Med"},{"key":"bibr41-09603271211066586","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-14926-4"},{"key":"bibr42-09603271211066586","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00117.2012"},{"key":"bibr43-09603271211066586","doi-asserted-by":"publisher","DOI":"10.1159\/000484314"},{"key":"bibr44-09603271211066586","doi-asserted-by":"publisher","DOI":"10.1016\/S1734-1140(12)70846-0"},{"key":"bibr45-09603271211066586","doi-asserted-by":"publisher","DOI":"10.4103\/0366-6999.225058"},{"key":"bibr46-09603271211066586","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.29.1756"},{"key":"bibr47-09603271211066586","doi-asserted-by":"publisher","DOI":"10.1186\/s12884-021-03650-7"},{"key":"bibr48-09603271211066586","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2020.06.015"},{"key":"bibr49-09603271211066586","doi-asserted-by":"publisher","DOI":"10.1002\/ddr.21596"},{"key":"bibr50-09603271211066586","doi-asserted-by":"publisher","DOI":"10.2147\/IJWH.S174734"},{"key":"bibr51-09603271211066586","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dev156"},{"key":"bibr52-09603271211066586","doi-asserted-by":"publisher","DOI":"10.1039\/D0FO01782E"},{"key":"bibr53-09603271211066586","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/7.2.205"}],"container-title":["Human &amp; Experimental Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/09603271211066586","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/09603271211066586","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/09603271211066586","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T07:45:19Z","timestamp":1777448719000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/09603271211066586"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,1]]},"references-count":53,"alternative-id":["10.1177\/09603271211066586"],"URL":"https:\/\/doi.org\/10.1177\/09603271211066586","relation":{},"ISSN":["0960-3271","1477-0903"],"issn-type":[{"value":"0960-3271","type":"print"},{"value":"1477-0903","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,1]]},"article-number":"09603271211066586"}}