{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T14:31:20Z","timestamp":1783953080042,"version":"3.55.0"},"reference-count":34,"publisher":"SAGE Publications","issue":"7","license":[{"start":{"date-parts":[[2021,1,27]],"date-time":"2021-01-27T00:00:00Z","timestamp":1611705600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"the Natural Science Basic Research Plan in Shaanxi Province of China","award":["Program No.2018JM7110"],"award-info":[{"award-number":["Program No.2018JM7110"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Hum Exp Toxicol"],"published-print":{"date-parts":[[2021,7]]},"abstract":"<jats:sec>\n                    <jats:title>Objective:<\/jats:title>\n                    <jats:p>C1q\/TNF-related protein 3 (CTRP3), a member of CTRP family, has been found to have neuroprotective effect. In the current study, we investigated the protective role of CTRP3 in hippocampal neurons exposed to oxygen-glucose deprivation\/reperfusion (OGD\/R).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and methods:<\/jats:title>\n                    <jats:p>\n                      The mRNA and protein levels of CTRP3 in OGD\/R-stimulated hippocampal neurons were measured using qRT-PCR and western blot analysis, respectively. CCK-8 assay was performed to assess cell viability. ROS production was measured using the fluorescence probe 2\u2032,7\u2032-dichlorofluorescein diacetate (H\n                      <jats:sub>2<\/jats:sub>\n                      DCFDA). The activities of SOD and GPx were determined using ELISA. Cell apoptosis was assessed. Luciferase reporter assay was carried out to assess the activation of ARE). The levels of p-AMPK and Nrf2 were measured using western blot.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>Our results showed that the expression of CTRP3 was significantly downregulated in hippocampal neuronal cells exposed to OGD\/R. Overexpression of CTRP3 improved cell viability of OGD\/R-induced hippocampal neurons. In addition, overexpression of CTRP3 attenuated the OGD\/R-caused oxidative stress with decreased ROS production and increased activities of SOD and GPx. Moreover, CTRP3 caused a significant increase in bcl-2 expression and decreases in bax expression and caspase-3 activity. Furthermore, CTRP3 overexpression significantly upregulated the levels of p-AMPK and Nrf2, as well induced the activation of ARE in OGD-R-induced hippocampal neurons. CTRP3 upregulated the mRNA expression levels of HO-1, NQO-1 and GPx-3. Additionally, treatment with the inhibitor of AMPK partially reversed the neuroprotective effect of CTRP3 in OGD\/R-exposed neurons.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>CTRP3 exerted protective effect on OGD\/R-induced cerebral injury, which was regulated by AMPK\/Nrf2\/ARE pathway.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/0960327121989412","type":"journal-article","created":{"date-parts":[[2021,1,27]],"date-time":"2021-01-27T05:06:41Z","timestamp":1611724001000},"page":"1153-1162","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":11,"title":["CTRP3 protects hippocampal neurons from oxygen-glucose deprivation-induced injury through the AMPK\/Nrf2\/ARE pathway"],"prefix":"10.1177","volume":"40","author":[{"given":"H","family":"Ding","sequence":"first","affiliation":[{"name":"Department of Anesthesiology, Shaanxi Provincial People\u2019s Hospital, Xi\u2019an, Shaanxi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, Shaanxi Provincial People\u2019s Hospital, Xi\u2019an, Shaanxi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5716-1698","authenticated-orcid":false,"given":"W","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, Shaanxi Provincial People\u2019s Hospital, Xi\u2019an, Shaanxi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2021,1,27]]},"reference":[{"key":"bibr1-0960327121989412","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2008.01.005"},{"issue":"11","key":"bibr2-0960327121989412","first-page":"1411","volume":"84","author":"Galkin A","year":"2019","journal-title":"Biochemistry"},{"key":"bibr3-0960327121989412","doi-asserted-by":"publisher","DOI":"10.1002\/brb3.959"},{"key":"bibr4-0960327121989412","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2015.08.020"},{"key":"bibr5-0960327121989412","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.1109766"},{"key":"bibr6-0960327121989412","doi-asserted-by":"publisher","DOI":"10.1016\/j.tem.2011.12.003"},{"key":"bibr7-0960327121989412","doi-asserted-by":"publisher","DOI":"10.1007\/s11154-013-9255-7"},{"key":"bibr8-0960327121989412","doi-asserted-by":"publisher","DOI":"10.1002\/cphy.c160044"},{"key":"bibr9-0960327121989412","doi-asserted-by":"publisher","DOI":"10.1016\/j.dsx.2019.04.047"},{"key":"bibr10-0960327121989412","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2016.02.001"},{"key":"bibr11-0960327121989412","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-019-1749-0"},{"key":"bibr12-0960327121989412","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2016.00237"},{"key":"bibr13-0960327121989412","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2016.11.016"},{"key":"bibr14-0960327121989412","doi-asserted-by":"publisher","DOI":"10.1038\/nrm.2017.95"},{"key":"bibr15-0960327121989412","doi-asserted-by":"publisher","DOI":"10.1111\/joim.12268"},{"key":"bibr16-0960327121989412","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/4717258"},{"key":"bibr17-0960327121989412","doi-asserted-by":"publisher","DOI":"10.1007\/s10557-018-6780-3"},{"key":"bibr18-0960327121989412","first-page":"315","volume":"108889","author":"Lee EH","year":"2020","journal-title":"Chem Biol Interact"},{"key":"bibr19-0960327121989412","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0178253"},{"key":"bibr20-0960327121989412","doi-asserted-by":"publisher","DOI":"10.1080\/21691401.2019.1666864"},{"key":"bibr21-0960327121989412","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbi.2020.108946"},{"key":"bibr22-0960327121989412","doi-asserted-by":"publisher","DOI":"10.2174\/1567202614666170306105315"},{"key":"bibr23-0960327121989412","doi-asserted-by":"publisher","DOI":"10.1007\/s00125-017-4232-4"},{"key":"bibr24-0960327121989412","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2019.109489"},{"key":"bibr25-0960327121989412","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-019-1760-5"},{"key":"bibr26-0960327121989412","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/7405602"},{"key":"bibr27-0960327121989412","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.28801"},{"key":"bibr28-0960327121989412","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2017.01.005"},{"key":"bibr29-0960327121989412","doi-asserted-by":"publisher","DOI":"10.1142\/S0192415X17500057"},{"key":"bibr30-0960327121989412","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2017.06.188"},{"key":"bibr31-0960327121989412","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2017.07.041"},{"key":"bibr32-0960327121989412","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.00118-16"},{"key":"bibr33-0960327121989412","doi-asserted-by":"publisher","DOI":"10.21037\/atm.2019.10.108"},{"key":"bibr34-0960327121989412","doi-asserted-by":"publisher","DOI":"10.18632\/aging.102534"}],"container-title":["Human &amp; Experimental Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0960327121989412","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/0960327121989412","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0960327121989412","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T07:47:03Z","timestamp":1777448823000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/0960327121989412"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,1,27]]},"references-count":34,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2021,7]]}},"alternative-id":["10.1177\/0960327121989412"],"URL":"https:\/\/doi.org\/10.1177\/0960327121989412","relation":{},"ISSN":["0960-3271","1477-0903"],"issn-type":[{"value":"0960-3271","type":"print"},{"value":"1477-0903","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,1,27]]}}}