{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T23:21:40Z","timestamp":1793575300516,"version":"4.5.0"},"reference-count":27,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Natural science foundation of Shanxi science and technology department","award":["201601D011104"],"award-info":[{"award-number":["201601D011104"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Hum Exp Toxicol"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:p>Spinal cord injury (SCI) is a destructive event in central nervous system (CNS) with the hallmark of deficits in neuronal function. Phoenixin-14 (PNX-14) is a reproductive peptide that also has neuroprotective effects. However, the role of PNX-14 in SCI has not yet been studied. In this study, we firstly investigated the effects of PNX-14 on the recovery of neurological dysfunction and microglial polarization in a SCI mice model. We demonstrated that PNX-14 improved the recovery of neurological dysfunction with increased Basso Mouse Scale (BMS) scores, reduced lesion area volume and Evans blue (EB) dye extravasation. PNX-14 alleviated neuronal apoptosis and neuroinflammation in mice underwent SCI. In vitro co-culture assay proved that PNX-14 protected neurons injury in response to LPS- activated BV-2 cells. PNX-14 suppressed the LPS- induced microglia M1 phenotype polarization with decreased expression of M1-associated markers (CD16 and iNOS) and increased expression of M2-associated markers (CD206 and Arg1). PNX-14 also suppressed LPS- caused decrease in anti-inflammatory cytokines TGF-\u03b2, IL-10, and IL-13, as well increase in pro-inflammatory cytokines TNF-\u03b1, IL-1\u03b2, and IL-6 in BV2 cells. PNX-14 treatment caused increased PTEN expression and decreased p-Akt expression in BV2 cells against LPS induction. While inhibition of PTEN by SF1670 reversed the effects of PNX-14 on LPS- induced phenotypic transition of BV2 cells. Taken together, we found that PNX-14 exerted protective effects on neurological dysfunction and inflammation in SCI mice through modulating microglial polarization via PTEN\/Akt signaling pathway.<\/jats:p>","DOI":"10.1177\/09603271221111345","type":"journal-article","created":{"date-parts":[[2022,8,14]],"date-time":"2022-08-14T02:13:47Z","timestamp":1660443227000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":15,"title":["Phoenixin-14 Promotes the Recovery of Neurological Dysfunction After Spinal Cord Injury by Regulating Microglial Polarization\n                    <i>via<\/i>\n                    PTEN\/Akt Signaling Pathway"],"prefix":"10.1177","volume":"41","author":[{"given":"Zhiyong","family":"Yu","sequence":"first","affiliation":[{"name":"Department of Orthopedics, Fuyong People\u2019s Hospital, Shenzhen, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Shanxi Bethune Hospital Shanxi Academy of Medical Sciences, Taiyuan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3234-6829","authenticated-orcid":false,"given":"Yonghong","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Shanxi Bethune Hospital Shanxi Academy of Medical Sciences, Taiyuan, China"},{"name":"Department of Neurosurgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2022,8,13]]},"reference":[{"key":"bibr1-09603271221111345","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(02)07603-1"},{"key":"bibr2-09603271221111345","doi-asserted-by":"publisher","DOI":"10.1172\/JCI90608"},{"key":"bibr3-09603271221111345","doi-asserted-by":"crossref","first-page":"281","DOI":"10.55782\/ane-2011-1848","volume":"71","author":"Oyinbo CA","year":"2011","journal-title":"Acta Neurobiol Exp (Wars)"},{"key":"bibr4-09603271221111345","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/9476020"},{"key":"bibr5-09603271221111345","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-021-02337-2"},{"key":"bibr6-09603271221111345","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2015.08.064"},{"key":"bibr7-09603271221111345","doi-asserted-by":"publisher","DOI":"10.2174\/0929866524666170207154417"},{"key":"bibr8-09603271221111345","first-page":"21","author":"Billert M","year":"2020","journal-title":"Int J Mol Sci"},{"key":"bibr9-09603271221111345","doi-asserted-by":"publisher","DOI":"10.1007\/s12640-020-00225-w"},{"key":"bibr10-09603271221111345","doi-asserted-by":"publisher","DOI":"10.1038\/s41420-020-00333-8"},{"key":"bibr11-09603271221111345","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-018-1193-6"},{"key":"bibr12-09603271221111345","doi-asserted-by":"publisher","DOI":"10.1016\/S1046-2023(02)00358-4"},{"key":"bibr13-09603271221111345","doi-asserted-by":"publisher","DOI":"10.1007\/s13311-018-0631-6"},{"key":"bibr14-09603271221111345","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awl296"},{"key":"bibr15-09603271221111345","doi-asserted-by":"publisher","DOI":"10.1016\/0166-2236(96)10049-7"},{"key":"bibr16-09603271221111345","doi-asserted-by":"publisher","DOI":"10.1007\/s00011-020-01355-9"},{"key":"bibr17-09603271221111345","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2020.108411"},{"key":"bibr18-09603271221111345","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2020.108275"},{"key":"bibr19-09603271221111345","doi-asserted-by":"publisher","DOI":"10.1080\/13813455.2021.1981946"},{"key":"bibr20-09603271221111345","doi-asserted-by":"publisher","DOI":"10.2147\/DDDT.S258857"},{"key":"bibr21-09603271221111345","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a036780"},{"key":"bibr22-09603271221111345","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2019.02.001"},{"key":"bibr23-09603271221111345","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2014.02.006"},{"key":"bibr24-09603271221111345","doi-asserted-by":"publisher","DOI":"10.2174\/156800908784293659"},{"key":"bibr25-09603271221111345","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1601515"},{"key":"bibr26-09603271221111345","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1501441112"},{"key":"bibr27-09603271221111345","doi-asserted-by":"publisher","DOI":"10.1007\/s10787-019-00623-3"}],"container-title":["Human &amp; Experimental Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/09603271221111345","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/09603271221111345","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/09603271221111345","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T07:45:35Z","timestamp":1777448735000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/09603271221111345"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,1]]},"references-count":27,"alternative-id":["10.1177\/09603271221111345"],"URL":"https:\/\/doi.org\/10.1177\/09603271221111345","relation":{},"ISSN":["0960-3271","1477-0903"],"issn-type":[{"value":"0960-3271","type":"print"},{"value":"1477-0903","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,1]]},"article-number":"09603271221111345"}}