{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T01:16:59Z","timestamp":1790126219416,"version":"4.0.1"},"reference-count":37,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Innovation Capacity Improvement Plan of Hebei Province","award":["20577705D"],"award-info":[{"award-number":["20577705D"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Hum Exp Toxicol"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To investigate the diagnostic efficiency of miR-222\u20133p in plasma exosomes (Exos) and plasma for preeclampsia (PE) and the effect of miR-222\u20133p targeting STMN1 in PE<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>MiR-222\u20133p levels in total plasma and plasma Exos were detected in PE patients and healthy controls. A bioinformatics database and dual-luciferase reporter assay were employed to verify the targeting relationship between miR-222\u20133p and STMN1. Trophoblast HTR-8\/Svneo cells were transfected with miR-222\u20133p inhibitors with\/without STMN1 shRNA, followed by MTT, wound healing and Transwell invasion assays. The mRNA and protein expressions were measured by qRT\u2012PCR and Western blotting, respectively.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>MiR-222\u20133p levels in total plasma and plasma Exos were higher in PE patients than in healthy controls, particularly in severe PE patients. In addition, miR-222\u20133p levels in total plasma and plasma Exos from PE patients were positively correlated with diastolic and systolic blood pressure. The area under the curve (AUC) of miR-222\u20133p in total plasma for PE diagnostic efficiency was 0.798, with a sensitivity of 76.67% and specificity of 71.93%, while the AUC of miR-222\u20133p in plasma Exos was 0.708 (sensitivity: 61.67%; specificity: 78.95%). In vitro, miR-222\u20133p targeted STMN1 in HTR-8\/Svneo cells. Low miR-222\u20133p expression reversed the inhibitory effect of STMN1 shRNA on the proliferation, invasion and migration of HTR\/SVneo cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>PE patients had increased miR-222\u20133p expression in total plasma and plasma Exos, which both have high diagnostic efficiency for PE. MiR-222\u20133p can target STMN1 to promote the proliferation, invasion and migration of HTR-8\/Svneo cells and is a potential therapeutic target of PE.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/09603271221138550","type":"journal-article","created":{"date-parts":[[2022,12,7]],"date-time":"2022-12-07T04:04:08Z","timestamp":1670385848000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":7,"title":["Dysregulated miR-222-3p in plasma exosomes of preeclampsia patients and its\n                    <i>In vitro<\/i>\n                    effect on HTR8\/SVneo extravillous trophoblast cells by targeting STMN1"],"prefix":"10.1177","volume":"41","author":[{"given":"Li-Ping","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynaecology, The Second Hospital of Hebei Medical University, Shijiazhuang, 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