{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T05:56:45Z","timestamp":1778219805017,"version":"3.51.4"},"reference-count":27,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2023,3,1]],"date-time":"2023-03-01T00:00:00Z","timestamp":1677628800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Xi`an Association for Science Technology","award":["095920221355"],"award-info":[{"award-number":["095920221355"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82204172"],"award-info":[{"award-number":["82204172"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Research Program of Science and Technology in Xi\u2019an City","award":["21YXYJ0122"],"award-info":[{"award-number":["21YXYJ0122"]}]},{"DOI":"10.13039\/501100007128","name":"Natural Science Foundation of Shaanxi Province","doi-asserted-by":"publisher","award":["2021JQ-070"],"award-info":[{"award-number":["2021JQ-070"]}],"id":[{"id":"10.13039\/501100007128","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Hum Exp Toxicol"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:p>This study evaluated the effect of fibroblast growth factor receptor 3 (FGFR3) on damaged hypertrophic chondrocytes of Kashin\u2013Beck disease (KBD). Immunohistochemical staining was used to evaluate FGFR3 expression in growth plates from KBD rat models and engineered cartilage. In vitro study, hypertrophic chondrocytes were pretreated by FGFR3 binding inhibitor (BGJ398) for 24\u00a0h before incubation at different T-2 toxin concentrations. Differentiation -related genes (Runx2, Sox9, and Col \u2169) and ECM degradation -related genes (MMP-13, Col \u2161) in the hypertrophic chondrocytes were analyzed using RT-PCR, and the corresponding proteins were analyzed using western blotting. Hypertrophic chondrocytes death was detected by the Annexin V\/PI double staining assay. The integrated optical density of FGFR3 staining was increased in knee cartilage of rats and engineered cartilage treated with T-2 toxin. Both protein and mRNA levels of Runx2, Sox9, Col \u2161, and Col \u2169 were decreased in a dose-dependent manner when exposed to the T-2 toxin and significantly upregulated by 1\u00a0\u03bcM BGJ398. The expression of MMP-1, MMP-9, and MMP-13 increased in a dose-dependent manner when exposed to T-2 toxin and significantly reduced by 1\u00a0\u03bcM BGJ398. 1\u00a0\u03bcM BGJ398 could prevent early apoptosis and necrosis induced by the T-2 toxin. Inhibiting the FGFR3 signal could alleviate extracellular matrix degradation, abnormal chondrocytes differentiation, and excessive cell death in T-2 toxin-induced hypertrophic chondrocytes.<\/jats:p>","DOI":"10.1177\/09603271231219480","type":"journal-article","created":{"date-parts":[[2023,12,7]],"date-time":"2023-12-07T04:09:46Z","timestamp":1701922186000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["Increased FGFR3 is involved in T-2 toxin-induced lesions of hypertrophic cartilage associated with endemic osteoarthritis"],"prefix":"10.1177","volume":"42","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5885-0214","authenticated-orcid":false,"given":"Ying","family":"Zhang","sequence":"first","affiliation":[{"name":"School of Public Health, Xi\u2019an Jiaotong University Health Science Center, Key Laboratory of Trace Elements and Endemic Diseases, National Health and Family Planning Commission, Xi\u2019an, China"},{"name":"School of Nursing, Health Science Center, Xi\u2019an Jiaotong University, Xi\u2019an, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qian","family":"Fang","sequence":"additional","affiliation":[{"name":"School of Public Health, Xi\u2019an Jiaotong University Health Science Center, Key Laboratory of Trace Elements and Endemic Diseases, National Health and Family Planning Commission, Xi\u2019an, China"},{"name":"Lanzhou Center for Disease Control and Prevention, Lanzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yinan","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Public Health, Xi\u2019an Jiaotong University Health Science Center, Key Laboratory of Trace Elements and Endemic Diseases, National Health and Family Planning Commission, Xi\u2019an, 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