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LPS is frequently of concern in medicine because of nearly all severe sepsis patients have elevated LPS plasma levels, which cause life-threatening organ dysfunction. Consequently, the potential protective benefit of acriflavine (Ac) in limiting LPS-induced acute inflammatory response and the possible underlying mechanisms were investigated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Male\n                      <jats:italic toggle=\"yes\">a<\/jats:italic>\n                      <jats:italic toggle=\"yes\">lbino<\/jats:italic>\n                      mice were treated with\n                      <jats:italic toggle=\"yes\">i.p<\/jats:italic>\n                      . Ac 4 or 8\u00a0mg\/kg\/day for 2\u00a0weeks, then received a single\n                      <jats:italic toggle=\"yes\">i.p<\/jats:italic>\n                      . LPS (10\u00a0mg\/kg) at day 14.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Ac administration ameliorated hepatic, pulmonary, and testicular dysfunction, as confirmed by attenuation of pathological changes and amendment of oxidative stress parameters. This was associated with inhibition of protein kinase R-like endoplasmic reticulum kinase\/phosphatidylinositol 3-kinase (PERK\/PI3K) endoplasmic reticulum (ER) stress pathway; toll-like receptor 4\/nuclear factor kappa B (TLR4\/NF-\u03baB) and active caspase-1\/gasdermin D (GSDMD)-N-terminal as well as interleukin-1 beta (IL-1\u03b2) inflammatory and pyroptotic signals.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Our results highlighted the protective potential of Ac in a mouse model of LPS-mediated systemic inflammatory response, which paves the way for its clinical application in sepsis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/09603271251361194","type":"journal-article","created":{"date-parts":[[2025,7,12]],"date-time":"2025-07-12T12:11:06Z","timestamp":1752322266000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["Acriflavine protects against LPS-induced sepsis via regulation of pyroptosis, inflammation, and endoplasmic reticulum stress"],"prefix":"10.1177","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3079-4441","authenticated-orcid":false,"given":"Esraa G.","family":"El-Waseif","sequence":"first","affiliation":[{"name":"Mansoura University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4421-3214","authenticated-orcid":false,"given":"Sara H.","family":"Hazem","sequence":"additional","affiliation":[{"name":"Mansoura University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0021-5533","authenticated-orcid":false,"given":"Dalia H.","family":"El-Kashef","sequence":"additional","affiliation":[{"name":"Mansoura University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3283-9471","authenticated-orcid":false,"given":"Ghada M.","family":"Suddek","sequence":"additional","affiliation":[{"name":"Mansoura University"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2025,7,12]]},"reference":[{"key":"e_1_3_6_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.71.110601.135414"},{"key":"e_1_3_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2020.109967"},{"key":"e_1_3_6_4_2","doi-asserted-by":"publisher","DOI":"10.1039\/C8RA04098B"},{"key":"e_1_3_6_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/aji.13527"},{"key":"e_1_3_6_6_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2016.0287"},{"key":"e_1_3_6_7_2","doi-asserted-by":"publisher","DOI":"10.1201\/9781003064961-55"},{"key":"e_1_3_6_8_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13613-023-01166-1"},{"key":"e_1_3_6_9_2","doi-asserted-by":"publisher","DOI":"10.3390\/cells13050439"},{"key":"e_1_3_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2017.01.013"},{"key":"e_1_3_6_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2013.07.008"},{"key":"e_1_3_6_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri2079"},{"key":"e_1_3_6_13_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20021790"},{"key":"e_1_3_6_14_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.00724"},{"key":"e_1_3_6_15_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.004184-0"},{"key":"e_1_3_6_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2009.04682.x"},{"key":"e_1_3_6_17_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.2c00573"},{"key":"e_1_3_6_18_2","doi-asserted-by":"publisher","DOI":"10.2174\/0929867327666200908114411"},{"key":"e_1_3_6_19_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0909353106"},{"key":"e_1_3_6_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgu004"},{"key":"e_1_3_6_21_2","doi-asserted-by":"publisher","DOI":"10.3390\/cancers13010102"},{"key":"e_1_3_6_22_2","first-page":"3549","article-title":"Antitumor activity of acriflavine in human hepatocellular carcinoma cells","volume":"34","author":"Lee CJ","year":"2014","unstructured":"Lee CJ, Yue CH, Lin YY, et al. 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