{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T20:22:09Z","timestamp":1777666929152,"version":"3.51.4"},"reference-count":28,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[1996,1,1]],"date-time":"1996-01-01T00:00:00Z","timestamp":820454400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Hum Exp Toxicol"],"published-print":{"date-parts":[[1996,1]]},"abstract":"<jats:p>1 The rational for administering hydroxocobalamin (OHCbl) as an antidote to cyanide poisoning is based on the high affinity ofCN ion for cobalt compounds. However, only few data are available on the influence of OHCbl on the intracellular cyanide pool.<\/jats:p>\n                  <jats:p>\n                    2 In human fibroblasts incubated for 10 min with 500 \u03bcM of [\n                    <jats:sup>14<\/jats:sup>\n                    C] cyanide, the accumulation ratio was 25 at 37\u00b0C (10.45 \u00b1 1.51 mM) and 11.9 at 4\u00b0C.\n                  <\/jats:p>\n                  <jats:p>3 Using the monoblastic U-937 cell line, a rapid uptake of radioactive cyanide was observed with a maximum accumulation ratio of 1.97 at 5 min.<\/jats:p>\n                  <jats:p>\n                    4 A linear relationship between cyanide uptake by U-937 cells and cyanide concentration in incubation medium (10-500 \u03bcM; 5 min) was found suggesting a first order process (k= 0.25 min\n                    <jats:sup>-1<\/jats:sup>\n                    ).\n                  <\/jats:p>\n                  <jats:p>5 After incubation of fibroblasts with 500 \u03bcM of OHCbl, a 75% decrease of intracellular cyanide was observed, with concomittant formation of intracellular cyanocobalamin CNCbl (intracellular\/extracellular ratio: 158).<\/jats:p>\n                  <jats:p>6 These findings suggest that OHCbl is able to penetrate into heavily cyanide loaded cells and to complex cyanide to the non-toxic CNCbl form.<\/jats:p>","DOI":"10.1177\/096032719601500104","type":"journal-article","created":{"date-parts":[[2008,12,24]],"date-time":"2008-12-24T01:08:04Z","timestamp":1230080884000},"page":"19-25","source":"Crossref","is-referenced-by-count":22,"title":["Complexation of intracellular cyanide by hydroxocobalamin using a human cellular model"],"prefix":"10.1177","volume":"15","author":[{"given":"Alain","family":"Astier","sequence":"first","affiliation":[{"name":"Laboratoire de Toxicologie, Service de Pharmacie, CHU Henri Mondor 94010 Cr\u00e9teil, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frederic J","family":"Baud","sequence":"additional","affiliation":[{"name":"R\u00e9animation Toxicologique, Hopital Fernand Widal, Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[1996,1,1]]},"reference":[{"key":"atypb1","unstructured":"Ballantyne B. Toxicology of cyanides, in Clinical and Experimental Toxicology of Cyanides , eds. Ballantyne B & Marrs TC, pp. 41-126, Bristol: Wrigth, 1987."},{"key":"atypb2","unstructured":"Marrs TC The choice of cyanide antidote, in Clinical and Experimental Toxicology of Cyanides, eds. Ballantyne B & Marrs TC, pp. 343-401, Bristol: Wrigth, 1987."},{"key":"atypb3","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1952.02930190015004"},{"key":"atypb4","unstructured":"Burrows GE & Way JL Cyanide intoxication in sheep: efficacy of sodium nitrite, sodium thiosulfate and cobaltous chloride. American Journal of Veterinary Research 1979; 40: 613-617."},{"key":"atypb5","doi-asserted-by":"publisher","DOI":"10.1007\/BF00293812"},{"key":"atypb6","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-81-19831"},{"key":"atypb7","first-page":"182","volume":"45","author":"Paulet G.","year":"1964","journal-title":"Archives des Maladies Professionnelles"},{"key":"atypb8","first-page":"455","volume":"23","author":"Lovatt Evans C.","year":"1964","journal-title":"Journal of Pharmacology"},{"key":"atypb9","first-page":"22","volume":"7","author":"Yacoub M.","year":"1974","journal-title":"Journal Europeen de Toxicology"},{"key":"atypb10","first-page":"90","volume":"24","author":"Pontal PG","year":"1982","journal-title":"Veterinary and Human Toxicology"},{"key":"atypb11","doi-asserted-by":"publisher","DOI":"10.1097\/00008506-199012000-00008"},{"key":"atypb12","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pa.24.040184.002315"},{"key":"atypb13","doi-asserted-by":"publisher","DOI":"10.1152\/ajplegacy.1907.19.2.223"},{"key":"atypb14","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.48.070179.004255"},{"key":"atypb15","unstructured":"Stoscheck CM Quantitation of proteins, in Methods in enzymology vol. 182: Guide to proteins purification , ed. Deutscher MP, pp. 62-63, San Diego: Academic Press, 1990."},{"key":"atypb16","unstructured":"Baskin SI, Wilkerson G., Alexander K. & Blitstein AG Cardiac effects of cyanide, in Clinical and Experimental Toxicology of Cyanides , eds. Ballantyne B & Marrs TC, pp. 138-155, Bristol: Wrigth, 1987."},{"key":"atypb17","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/S0022-3565(25)33013-2","volume":"221","author":"McMillan DE","year":"1982","journal-title":"Journal of Pharmacology and Experimental Therapeutic"},{"key":"atypb18","unstructured":"Duggard PH The absorption of cyanide through human skin in vitro from solutions of sodium cyanide and gaseous HCN, in Clinical and Experimental Toxicology of Cyanides , eds. Ballantyne B & Marrs TC, pp. 127-137, Bristol: Wrigth, 1987."},{"key":"atypb19","first-page":"51","volume":"15","author":"Ballantyne B.","year":"1975","journal-title":"Journal of Forensic Sciences"},{"key":"atypb20","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1985.tb02827.x"},{"key":"atypb21","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cb.04.110188.001103"},{"key":"atypb22","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V63.2.335.335"},{"key":"atypb23","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199112193252502"},{"key":"atypb24","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/0378-4347(95)00012-8","volume":"667","author":"A. Astier","year":"1995","journal-title":"Journal of Chromatography Biomedical Applications"},{"key":"atypb25","doi-asserted-by":"publisher","DOI":"10.1097\/00008506-199404000-00006"},{"key":"atypb26","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(61)90107-1"},{"key":"atypb27","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(78)90232-1"},{"key":"atypb28","unstructured":"Masakoa T. High dose methotrexate therapy with citrovorum factor rescue for acute leukemia and malignant lymphoma, in Methotrexate in Cancer Therapy, eds. K. Kimura and Y.M. Wang, pp. 173-178, New York: Raven Press, 1986."}],"container-title":["Human &amp; Experimental Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/096032719601500104","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/096032719601500104","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T07:44:51Z","timestamp":1777448691000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/096032719601500104"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,1]]},"references-count":28,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1996,1]]}},"alternative-id":["10.1177\/096032719601500104"],"URL":"https:\/\/doi.org\/10.1177\/096032719601500104","relation":{},"ISSN":["0960-3271","1477-0903"],"issn-type":[{"value":"0960-3271","type":"print"},{"value":"1477-0903","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,1]]}}}